caa a22 4500 463238664 CHVBK 20180405153306.0 cr unu---uuuuu 170326e20070701xx s 000 0 eng 10.1007/s11259-007-3518-2 doi (NATIONALLICENCE)springer-10.1007/s11259-007-3518-2 Plasma Concentrations of a Type II Collagen-derived Peptide and Its Nitrated Form in Growing Ardenner Sound Horses and in Horses Suffering from Juvenile Digital Degenerative Osteoarthropathy [Elektronische Daten] [J.-Ph. Lejeune, D. Serteyn, M. Gangl, N. Schneider, G. Deby-Dupont, M. Deberg, Y. Henrotin] Several breeds of draft horses suffer from degenerative digital osteoarthropathy, resulting in a reduced active lifespan. A group of 30 Ardenner horses was followed, in standardized conditions, from 15 to 28 months of age to detect the early manifestations of the disease. The severity of the disease was assessed according to a personal grading system including clinical and radiographic items. Coll 2-1, a peptide of the helical region of type II collagen, and its nitrated form (Coll 2-1 NO2) were assayed in blood plasma collected at 452 ± 18 days, 504 ± 20 days, 558 ± 18 days, 613 ± 19 days, 675 ± 19 days, 752 ± 21 days and 852 ± 19 days of age. At the end of the follow-up period, 53.3% of Ardenner horses were affected by a degenerative digital osteoarthropathy. A significant effect (p < 0.05) of time, sex and pathology was observed for Coll 2-1 NO2. Variations of Coll 2-1 were not significant except for the time effect. The elevation of Coll 2-1 NO2 in the pathological group could indicate an inflammatory process during the growth of the affected horses, as nitration of tyrosine is mediated through reactive oxygen/nitrogen species and/or myeloperoxidase activity. Coll 2-1 NO2 appears to be an interesting early marker of cartilage degradation and oxidation in degenerative osteoarthropathy. Springer Science + Business Media, Inc., 2007 Ardenner horses nationallicence type II collagen nationallicence degenerative digital osteoarthropathy nationallicence AAEP : American Association of Equine Practitioners nationallicence BSA : bovine serum albumin nationallicence Coll 2-1 NO2 : nitrate Coll 2-1 nationallicence COMP : cartilage oligomeric matrix protein nationallicence CPII : carboxypeptide type II nationallicence ECM : extracellular matrix nationallicence EDTA : ethylenediaminetetraacetic acid nationallicence KS : keratan sulphate nationallicence LSM : least-squares mean nationallicence MMP : matrix metalloproteinase nationallicence MPO : myeloperoxidase nationallicence OA : osteoarthritis nationallicence PBS : phosphate-buffered saline nationallicence ROS : reactive oxygen species nationallicence Lejeune J.-Ph Centre Européen du Cheval, Mont-le-Soie, Vielsalm aut Serteyn D. Centre Européen du Cheval, Mont-le-Soie, Vielsalm aut Gangl M. Department of Clinical Sciences, Large Animal Surgery, Faculty of Veterinary Medicine, University of Liège, Liège aut Schneider N. Centre Européen du Cheval, Mont-le-Soie, Vielsalm aut Deby-Dupont G. Center for Oxygen Research and Development, University of Liège, Liège aut Deberg M. Bone and Cartilage Research Unit, University of Liège, Institute of Pathology, Liège, Belgium aut Henrotin Y. Center for Oxygen Research and Development, University of Liège, Liège aut Veterinary Research Communications Kluwer Academic Publishers 31/5(2007-07-01), 591-601 0165-7380 31:5<591 2007 31 11259 https://doi.org/10.1007/s11259-007-3518-2 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11259-007-3518-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Lejeune J.-Ph Centre Européen du Cheval, Mont-le-Soie, Vielsalm aut NATIONALLICENCE 700 1- Serteyn D. Centre Européen du Cheval, Mont-le-Soie, Vielsalm aut NATIONALLICENCE 700 1- Gangl M. Department of Clinical Sciences, Large Animal Surgery, Faculty of Veterinary Medicine, University of Liège, Liège aut NATIONALLICENCE 700 1- Schneider N. Centre Européen du Cheval, Mont-le-Soie, Vielsalm aut NATIONALLICENCE 700 1- Deby-Dupont G. Center for Oxygen Research and Development, University of Liège, Liège aut NATIONALLICENCE 700 1- Deberg M. Bone and Cartilage Research Unit, University of Liège, Institute of Pathology, Liège, Belgium aut NATIONALLICENCE 700 1- Henrotin Y. Center for Oxygen Research and Development, University of Liège, Liège aut NATIONALLICENCE 773 0- Veterinary Research Communications Kluwer Academic Publishers 31/5(2007-07-01), 591-601 0165-7380 31:5<591 2007 31 11259 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer