caa a22 4500 463238680 CHVBK 20180405153306.0 cr unu---uuuuu 170326e20070701xx s 000 0 eng 10.1007/s11259-007-3510-x doi (NATIONALLICENCE)springer-10.1007/s11259-007-3510-x Development of Indirect Enzyme-linked Immunosorbent Assay with Nucleoprotein as Antigen for Detection and Quantification of Antibodies against Avian Influenza Virus [Elektronische Daten] [R. Wu, S. Hu, Y. Xiao, Z. Li, D. Shi, D. Bi] Avian influenza (AI) is a serious infectious disease caused by avian influenza virus (AIV) belonging to type A Orthomyxovirus. In the present study, we developed an indirect enzyme-linked immunosorbent assay (ELISA) employing E. coli-expressed full-length nucleoprotein (NP) of H9N2 avian influenza virus for the detection and quantification of antibodies against AIV nucleoprotein. The NP-ELISA was compared with the AI agar gel propagation (AGP) test, haemagglutination inhibition (HI) test, and IDEXX-FlockChek ELISA using 263 sera. The NP-ELISA was significantly more sensitive than the AGP and HI tests, and showed 96.2% agreement ratio with IDEXX-FlockChek ELISA. With results obtained using the NP-ELISA, an ELISA titre (ET) prediction equation, with which the ELISA titres of a flock or individual chickens can be determined, was derived from a positive/negative (P/N) ratio standard curve. The NP-ELISA enables an alternative rapid serological diagnosis and is suitable for influenza A antibody screening, especially in species that harbour several influenza subtypes. Springer Science + Business Media, Inc., 2007 avian influenza virus nationallicence full-length nucleoprotein nationallicence NP-ELISA nationallicence detection and quantification of antibodies nationallicence AGP : agar gel propagation nationallicence AI : avian influenza nationallicence AIV : avian influenza virus nationallicence BSA : bovine serum albumin nationallicence ELISA : enzyme-linked immunosorbent assay nationallicence ET : ELISA titre nationallicence GST : glutathione S-transferase nationallicence HA : haemagglutinin nationallicence HI : haemagglutination inhibition nationallicence IgG : immunoglobulin G nationallicence IPTG : isopropylthio-β-d-galactoside nationallicence NA : neuraminidase nationallicence NP : nucleoprotein nationallicence OD : optical density nationallicence PBS : phosphate-buffered saline nationallicence P/N : positive/negative nationallicence SDS-PAGE : sodium dodecyl sulfate-polyacrylamide gel electrophoresis nationallicence Wu R. Laboratory of Animal Microbiology and Immunology, State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, People's Republic of China aut Hu S. Laboratory of Animal Microbiology and Immunology, State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, People's Republic of China aut Xiao Y. Laboratory of Animal Microbiology and Immunology, State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, People's Republic of China aut Li Z. Laboratory of Animal Microbiology and Immunology, State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, People's Republic of China aut Shi D. Laboratory of Animal Microbiology and Immunology, State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, People's Republic of China aut Bi D. Laboratory of Animal Microbiology and Immunology, State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, People's Republic of China aut Veterinary Research Communications Kluwer Academic Publishers 31/5(2007-07-01), 631-641 0165-7380 31:5<631 2007 31 11259 https://doi.org/10.1007/s11259-007-3510-x text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11259-007-3510-x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Wu R. Laboratory of Animal Microbiology and Immunology, State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, People's Republic of China aut NATIONALLICENCE 700 1- Hu S. Laboratory of Animal Microbiology and Immunology, State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, People's Republic of China aut NATIONALLICENCE 700 1- Xiao Y. Laboratory of Animal Microbiology and Immunology, State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, People's Republic of China aut NATIONALLICENCE 700 1- Li Z. Laboratory of Animal Microbiology and Immunology, State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, People's Republic of China aut NATIONALLICENCE 700 1- Shi D. Laboratory of Animal Microbiology and Immunology, State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, People's Republic of China aut NATIONALLICENCE 700 1- Bi D. Laboratory of Animal Microbiology and Immunology, State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, People's Republic of China aut NATIONALLICENCE 773 0- Veterinary Research Communications Kluwer Academic Publishers 31/5(2007-07-01), 631-641 0165-7380 31:5<631 2007 31 11259 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer