caa a22 4500 463238729 CHVBK 20180405153306.0 cr unu---uuuuu 170326e20070201xx s 000 0 eng 10.1007/s11259-006-3429-7 doi (NATIONALLICENCE)springer-10.1007/s11259-006-3429-7 Stimulation of the In Vitro Migration of Ovine Eosinophils by Factors Derived from the Sheep Scab Mite, Psoroptes ovis [Elektronische Daten] [L. Wildblood, D. Jones] The ectoparasitic astigmatid mite Psoroptes ovis causes sheep scab, a highly contagious, severe allergic dermatitis associated with damage to the fleece and hide, loss of condition and occasional mortality. The scab lesion is characterized by a massive infiltration of eosinophils that begins very rapidly after infection. This paper reports the finding that mite-derived factors directly enhance the migration of ovine eosinophils in vitro. Significant (p < 0.01) and dose-dependent (r = 0.972 ± 0.018 (SD)) activity was initially identified in whole mite extracts, by comparison with medium controls in an assay based on modified Boyden chambers and ovine bone marrow target cells. Similar pro-migratory activity (p < 0.005; r = 0.928 ± 0.069 (SD)) was detected in washes containing mite excretory/secretory material. By direct comparison with migration ratios (n = 3) for defined chemotactic (rmeotaxin = 3.430 ± 0.360 (SD)) and chemokinetic (rminterleukin-5 = 0.982 ± 0.112 (SD)) stimuli it was determined that the activity in both mite extracts (0.992 ± 0.038 (SD)) and mite washes (0.969 ± 0.071 (SD)) was chemokinetic. Subsequent experiments (n = 3) in which live mites were incorporated directly into the in vitro assay system indicated that they produced factors that significantly (p < 0.001) enhanced eosinophil migration to a degree directly related to mite numbers (r = 0.993 ± 0.005 (SD)). The identity of the factor(s) responsible is uncertain, but their presence suggests that mites may be capable of directly activating eosinophils in vivo, and raises the possibility that mites could directly influence, perhaps even initiate, the rapid early tissue eosinophilic response observed in experimental sheep scab infections. Springer Science + Business Media, Inc., 2007 ectoparasites nationallicence eosinophils nationallicence excretory/secretory products nationallicence in vitro chemotaxis/chemokinesis nationallicence mites nationallicence Psoroptes ovis nationallicence sheep scab nationallicence FCS : fetal calf serum nationallicence HBSS : Hanks balanced salt solution nationallicence IL-5 : interleukin-5 nationallicence IMDM : Iscove's modified Dulbecco's medium nationallicence PBS : phosphate-buffered saline nationallicence r : correlation coefficient nationallicence rm : recombinant murine nationallicence SBMC : sheep bone marrow cells nationallicence SD : standard deviation nationallicence SDS : sodium dodecyl sulphate nationallicence SEM : standard error of the mean nationallicence Wildblood L. Moredun Research Institute, Pentlands Science Park, Penicuik, Scotland, UK aut Jones D. Moredun Research Institute, Pentlands Science Park, Penicuik, Scotland, UK aut Veterinary Research Communications Kluwer Academic Publishers 31/2(2007-02-01), 197-206 0165-7380 31:2<197 2007 31 11259 https://doi.org/10.1007/s11259-006-3429-7 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11259-006-3429-7 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Wildblood L. Moredun Research Institute, Pentlands Science Park, Penicuik, Scotland, UK aut NATIONALLICENCE 700 1- Jones D. Moredun Research Institute, Pentlands Science Park, Penicuik, Scotland, UK aut NATIONALLICENCE 773 0- Veterinary Research Communications Kluwer Academic Publishers 31/2(2007-02-01), 197-206 0165-7380 31:2<197 2007 31 11259 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer