caa a22 4500 46323877X CHVBK 20180405153306.0 cr unu---uuuuu 170326e20070201xx s 000 0 eng 10.1007/s11259-006-3330-4 doi (NATIONALLICENCE)springer-10.1007/s11259-006-3330-4 Immunohistochemical Study of Phospho-Stat3-ser727 Expression in Feline Mammary Gland Tumours [Elektronische Daten] [C. Petterino, G. Podestà, A. Ratto, M. Drigo, C. Pellegrino] We describe the expression of pStat3-ser727 (signal transducer and activator of transcription 3 phosphorylated on serine 727) in normal, hyperplastic and neoplastic feline mammary gland tissue assessed by immunohistochemistry in 56 cats. The samples included 4 normal mammary non-lactating tissues, 13 hyperplastic lesions (9 lobular and 4 fibroepithelial) and 39 tumours (6 benign and 33 carcinomas). For immunohistochemistry, tissue sections were incubated with anti-pStat3-ser727 monoclonal antibodies and visualized with EnVision-DAB polymer. pStat3-ser727 positivity was quantified in a semi-quantitative manner, differentiating cytoplasmic and nuclear localization. Intense anti-pStat3-ser727 immunoreactivity was detected in epithelial neoplastic cells and in the fibro-component in two fibroepithelial hyperplastic lesions. The immunostaining was dot-like in the cytoplasm and homogeneous in the nuclei in both benign and malignant lesions. Statistically significant relations were observed between nuclear expression of pStat3-ser727 and the pleomorphism score (p = 0.006), mitotic activity (p < 0.0001), and histological grade (p < 0.0001). In contrast, no significant correlations were observed for cytoplasmic pStat3-ser727. These findings add new and interesting information on the potential role of the phosphorylated form of Stat3 in malignant lesions. Springer Science + Business Media, Inc., 2007 Stat3 nationallicence serine-727 nationallicence feline nationallicence mammary carcinoma nationallicence tumour nationallicence hyperplasia nationallicence BSA : bovine serum albumin nationallicence CSF-1 : colony-stimulating factor-1 nationallicence DAB : 3,3′-diaminobenzide tetrahydrochloride nationallicence EGFr : epidermal growth factor receptor nationallicence IFN : interferon nationallicence FMLP : N-formyl-methionyl-leucyl-phenylalanine nationallicence MAPK : mitogen-activated protein kinase nationallicence PBS : phosphate-buffered saline nationallicence PDGFR : platelet-derived growth factor receptor nationallicence pStat3-ser727 : phosphorylated signal transducer and activator of transcription 3-serine 727 nationallicence Stat3 : signal transducers and activators of transcription 3 nationallicence TBS : Tris-buffered saline nationallicence TNF : tumour necrosis factor nationallicence WHO : World Health Organization nationallicence Petterino C. Department of Public Health, Comparative Pathology and Veterinary Hygiene School of Veterinary Medicine, University of Padua, Agripolis, Legnaro aut Podestà G. Istituto Zooprofilattico Sperimentale del Piemonte, Liguria e Valle D'Aosta, National Reference Centre of Veterinary and Comparative Oncology, Genoa, Italy aut Ratto A. Istituto Zooprofilattico Sperimentale del Piemonte, Liguria e Valle D'Aosta, National Reference Centre of Veterinary and Comparative Oncology, Genoa, Italy aut Drigo M. Department of Public Health, Comparative Pathology and Veterinary Hygiene School of Veterinary Medicine, University of Padua, Agripolis, Legnaro aut Pellegrino C. Istituto Zooprofilattico Sperimentale del Piemonte, Liguria e Valle D'Aosta, National Reference Centre of Veterinary and Comparative Oncology, Genoa, Italy aut Veterinary Research Communications Kluwer Academic Publishers 31/2(2007-02-01), 173-184 0165-7380 31:2<173 2007 31 11259 https://doi.org/10.1007/s11259-006-3330-4 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11259-006-3330-4 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Petterino C. Department of Public Health, Comparative Pathology and Veterinary Hygiene School of Veterinary Medicine, University of Padua, Agripolis, Legnaro aut NATIONALLICENCE 700 1- Podestà G. Istituto Zooprofilattico Sperimentale del Piemonte, Liguria e Valle D'Aosta, National Reference Centre of Veterinary and Comparative Oncology, Genoa, Italy aut NATIONALLICENCE 700 1- Ratto A. Istituto Zooprofilattico Sperimentale del Piemonte, Liguria e Valle D'Aosta, National Reference Centre of Veterinary and Comparative Oncology, Genoa, Italy aut NATIONALLICENCE 700 1- Drigo M. Department of Public Health, Comparative Pathology and Veterinary Hygiene School of Veterinary Medicine, University of Padua, Agripolis, Legnaro aut NATIONALLICENCE 700 1- Pellegrino C. Istituto Zooprofilattico Sperimentale del Piemonte, Liguria e Valle D'Aosta, National Reference Centre of Veterinary and Comparative Oncology, Genoa, Italy aut NATIONALLICENCE 773 0- Veterinary Research Communications Kluwer Academic Publishers 31/2(2007-02-01), 173-184 0165-7380 31:2<173 2007 31 11259 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer