caa a22 4500 463239865 CHVBK 20180405153309.0 cr unu---uuuuu 170326e20070701xx s 000 0 eng 10.1007/s10947-007-0112-y doi (NATIONALLICENCE)springer-10.1007/s10947-007-0112-y Conformation changes in the DNA molecule caused by γ-irradiation of its water ethanol solutions of great ionic strength [Elektronische Daten] [S. Paston, I. Zyryanova, Yu. Zaichikova, V. Zamotin] The conformation changes in DNA in water ethanol solutions (ethanol content 0-22 wt.%, ionic strength μ = 0.15 M NaCl) exposed to γ radiation (10 Gy and 30 Gy doses) were investigated by low-gradient viscosimetry, birefringence in a flow, circular dichroism (CD), and UV spectrophotometry. For µ = 0.15 M, the volume of intact DNA remained constant over the whole range of alcohol concentrations studied in contrast to water alcohol solutions of lower ionic strength, in which the specific volume of the macromolecule decreased cooperatively at a critical concentration of alcohol corresponding to a structure disturbance of water (C cr = 16.5 wt.% for ethanol). Inversion of the dependence of the specific volume of DNA on the radiation dose, which was found previously for water alcohol solutions with µ = 0.003 M NaCl, vanished under these conditions. For µ = 0.15 M, the dependence of V sp of DNA on C eth had no features at C eth = C cr; at C eth ≥ 1.7 wt.% the volume of the macromolecule did not change after irradiation with doses of up to 40 Gy. This is probably explained by the fact that at high salt concentrations the alcohol loses its influence on the structure of the solvent, and its role in the process of γ irradiation is restricted to interception of the active products of water radiolysis. Springer Science+Business Media, Inc., 2007 DNA conformation nationallicence water alcohol solutions nationallicence γ radiation nationallicence Paston S. Physical Faculty, St. Petersburg State University, Russia aut Zyryanova I. Physical Faculty, St. Petersburg State University, Russia aut Zaichikova Yu Physical Faculty, St. Petersburg State University, Russia aut Zamotin V. Physical Faculty, St. Petersburg State University, Russia aut Journal of Structural Chemistry Springer US 48/4(2007-07-01), 734-739 0022-4766 48:4<734 2007 48 10947 https://doi.org/10.1007/s10947-007-0112-y text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10947-007-0112-y text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Paston S. Physical Faculty, St. Petersburg State University, Russia aut NATIONALLICENCE 700 1- Zyryanova I. Physical Faculty, St. Petersburg State University, Russia aut NATIONALLICENCE 700 1- Zaichikova Yu Physical Faculty, St. Petersburg State University, Russia aut NATIONALLICENCE 700 1- Zamotin V. Physical Faculty, St. Petersburg State University, Russia aut NATIONALLICENCE 773 0- Journal of Structural Chemistry Springer US 48/4(2007-07-01), 734-739 0022-4766 48:4<734 2007 48 10947 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer