caa a22 4500 463247280 CHVBK 20180405153329.0 cr unu---uuuuu 170326e20071201xx s 000 0 eng 10.1007/s10086-007-0890-z doi (NATIONALLICENCE)springer-10.1007/s10086-007-0890-z Hybrid aspen with a transgene for fungal manganese peroxidase is a potential contributor to phytoremediation of the environment contaminated with bisphenol A [Elektronische Daten] [Yosuke Iimura, Madoka Yoshizumi, Tomonori Sonoki, Mikiko Uesugi, Kenji Tatsumi, Ken-ichi Horiuchi, Shinya Kajita, Yoshihiro Katayama] To assess the possible utility of a fungal gene for manganese-dependent peroxidase (MnP) produced by a transgenic plant in phytoremediation, we transformed hybrid aspen with a chimeric gene for MnP. Our gene construct allowed expression of the gene for MnP in plants and relatively high MnP activity was detected in the hydroponic medium in which roots of plants that expressed the transgene had been cultured. Some of our transgenic plants were able to remove bisphenol A from the medium more efficiently than wild-type plants. Our results demonstrate that, without any modification of the coding sequence, a chimeric gene for fungal MnP can be expressed in a woody plant, with secretion of active MnP from roots into the rhizosphere. Our strategy suggests new options using woody plants for phytoremediation. The Japan Wood Research Society, 2007 Bisphenol A nationallicence Manganese-dependent peroxidase nationallicence Phytoremediation nationallicence Populus nationallicence Trametes versicolor nationallicence Iimura Yosuke Research Institute for Environmental Management Technology, National Institute of Advanced Industrial Science and Technology, 305-8569, Tsukuba, Japan aut Yoshizumi Madoka Department of Environment Symbiotic Production Systems, Graduate School of Bio-Applications and Systems Engineering, Tokyo University of Agriculture and Technology, 184-8588, Koganei, Tokyo, Japan aut Sonoki Tomonori Research Institute for Environmental Management Technology, National Institute of Advanced Industrial Science and Technology, 305-8569, Tsukuba, Japan aut Uesugi Mikiko Department of Environment Symbiotic Production Systems, Graduate School of Bio-Applications and Systems Engineering, Tokyo University of Agriculture and Technology, 184-8588, Koganei, Tokyo, Japan aut Tatsumi Kenji Research Institute for Environmental Management Technology, National Institute of Advanced Industrial Science and Technology, 305-8569, Tsukuba, Japan aut Horiuchi Ken-ichi Core Project, Technical Center, Nitta Corporation, 639-1085, Yamatokoriyama, Japan aut Kajita Shinya Department of Environment Symbiotic Production Systems, Graduate School of Bio-Applications and Systems Engineering, Tokyo University of Agriculture and Technology, 184-8588, Koganei, Tokyo, Japan aut Katayama Yoshihiro Department of Environment Symbiotic Production Systems, Graduate School of Bio-Applications and Systems Engineering, Tokyo University of Agriculture and Technology, 184-8588, Koganei, Tokyo, Japan aut Journal of Wood Science Springer-Verlag 53/6(2007-12-01), 541-544 1435-0211 53:6<541 2007 53 10086 https://doi.org/10.1007/s10086-007-0890-z text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10086-007-0890-z text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Iimura Yosuke Research Institute for Environmental Management Technology, National Institute of Advanced Industrial Science and Technology, 305-8569, Tsukuba, Japan aut NATIONALLICENCE 700 1- Yoshizumi Madoka Department of Environment Symbiotic Production Systems, Graduate School of Bio-Applications and Systems Engineering, Tokyo University of Agriculture and Technology, 184-8588, Koganei, Tokyo, Japan aut NATIONALLICENCE 700 1- Sonoki Tomonori Research Institute for Environmental Management Technology, National Institute of Advanced Industrial Science and Technology, 305-8569, Tsukuba, Japan aut NATIONALLICENCE 700 1- Uesugi Mikiko Department of Environment Symbiotic Production Systems, Graduate School of Bio-Applications and Systems Engineering, Tokyo University of Agriculture and Technology, 184-8588, Koganei, Tokyo, Japan aut NATIONALLICENCE 700 1- Tatsumi Kenji Research Institute for Environmental Management Technology, National Institute of Advanced Industrial Science and Technology, 305-8569, Tsukuba, Japan aut NATIONALLICENCE 700 1- Horiuchi Ken-ichi Core Project, Technical Center, Nitta Corporation, 639-1085, Yamatokoriyama, Japan aut NATIONALLICENCE 700 1- Kajita Shinya Department of Environment Symbiotic Production Systems, Graduate School of Bio-Applications and Systems Engineering, Tokyo University of Agriculture and Technology, 184-8588, Koganei, Tokyo, Japan aut NATIONALLICENCE 700 1- Katayama Yoshihiro Department of Environment Symbiotic Production Systems, Graduate School of Bio-Applications and Systems Engineering, Tokyo University of Agriculture and Technology, 184-8588, Koganei, Tokyo, Japan aut NATIONALLICENCE 773 0- Journal of Wood Science Springer-Verlag 53/6(2007-12-01), 541-544 1435-0211 53:6<541 2007 53 10086 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer