caa a22 4500 463247477 CHVBK 20180405153330.0 cr unu---uuuuu 170326e20070601xx s 000 0 eng 10.1007/s10086-006-0843-y doi (NATIONALLICENCE)springer-10.1007/s10086-006-0843-y Transmission of mitochondrial plasmids in protoplast cell fusion between compatible monokaryons of Lentinula edodes [Elektronische Daten] [Masaki Fukuda, Izumi Sendo, Satoko Imahori, Yukitaka Fukumasa-Nakai] Electrophoretic analysis of the transmission pattern of mitochondrial plasmids in protoplast cell fusion between compatible monokaryons of Lentinula edodes indicates that three of the four plasmids carried in parental monokaryons are effectively transferred and replicated in the protoplast fusants. The two monokaryons, 1158a and 1569a, carried different plasmids that could be distinguished by a single restriction digest. Electrophoresis of intact plasmids and restriction analyses indicate that all but one of the fusants carry three of the four possible plasmids, indicating that transmission of plasmids in protoplast fusions is principally biparental in L. edodes. Thus, heterocytoplasmic cells of L. edodes can be effectively constructed by protoplast cell fusion. In addition, plasmids of the same homology group cannot coexist in the heteroplasmic cells after protoplast cell fusion. The Japan Wood Research Society, 2006 Mitochondrial plasmid nationallicence Protoplast cell fusion nationallicence Lentinula edodes nationallicence Fukuda Masaki Faculty of Agriculture, Shinshu University, 399-4598, Minamiminowa, Nagano, Japan aut Sendo Izumi Faculty of Agriculture, Shinshu University, 399-4598, Minamiminowa, Nagano, Japan aut Imahori Satoko Faculty of Agriculture, Shinshu University, 399-4598, Minamiminowa, Nagano, Japan aut Fukumasa-Nakai Yukitaka Tottori Mycological Institute, 689-1125, Tottori, Japan aut Journal of Wood Science Springer-Verlag 53/3(2007-06-01), 264-267 1435-0211 53:3<264 2007 53 10086 https://doi.org/10.1007/s10086-006-0843-y text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10086-006-0843-y text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Fukuda Masaki Faculty of Agriculture, Shinshu University, 399-4598, Minamiminowa, Nagano, Japan aut NATIONALLICENCE 700 1- Sendo Izumi Faculty of Agriculture, Shinshu University, 399-4598, Minamiminowa, Nagano, Japan aut NATIONALLICENCE 700 1- Imahori Satoko Faculty of Agriculture, Shinshu University, 399-4598, Minamiminowa, Nagano, Japan aut NATIONALLICENCE 700 1- Fukumasa-Nakai Yukitaka Tottori Mycological Institute, 689-1125, Tottori, Japan aut NATIONALLICENCE 773 0- Journal of Wood Science Springer-Verlag 53/3(2007-06-01), 264-267 1435-0211 53:3<264 2007 53 10086 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer