caa a22 4500 463247574 CHVBK 20180405153330.0 cr unu---uuuuu 170326e20070801xx s 000 0 eng 10.1007/s10086-006-0861-9 doi (NATIONALLICENCE)springer-10.1007/s10086-006-0861-9 Introduction of mitochondrial DNA from Pleurotus ostreatus into Pleurotus pulmonarius by interspecific protoplast fusion [Elektronische Daten] [Masaki Fukuda, Masayoshi Wakayama, Masayuki Uchida, Yukitaka Fukumasa-Nakai, Teruyuki Matsumoto] Interspecific protoplast fusion between two monokaryotic strains (a methionine auxotrophic and chloramphenicol-resistant Pleurotus ostreatus strain and a wild-type strain of Pleurotus pulmonarius) was carried out to introduce mitochondrial DNA (mtDNA) from P. ostreatus into P. pulmonarius. Because mycelial colonies regenerated on minimum medium containing chloramphenicol only after the treatment of protoplast electrofusion, the regenerants were regarded as protoplast fusants. The fusants isolated from regenerated colonies were uninucleate, and their resistance of chloramphenicol seemed to be a stable trait. The fusants mated with P. pulmonarius but not with P. ostreatus, and showed almost identical random amplified polymorphic DNA (RAPD) patterns to that of the parental P. pulmonarius monokaryon. The sizes of mitochondrial genomes were estimated to be 81.5 kb for P. ostreatus monokaryon, 54.9 kb for P. pulmonarius monokaryon, and 84.5 or 86.0 kb for the fusants. BamHI and EcoRI digest patterns of the fusant mtDNAs were almost the same as the parental P. ostreatus monokaryon. From the above results, the fusants seemed to carry nuclei derived from the monokaryon of P. pulmonarius and mtDNA including the chloramphenicol-resistance gene from the P. ostreatus monokaryon, suggesting that the P. ostreatus mtDNA had been introduced into P. pulmonarius cells by interspecific protoplast fusion. The Japan Wood Research Society, 2007 Protoplast fusion nationallicence Pleurotus ostreatus nationallicence Pleurotus pulmonarius nationallicence Mitochondrial DNA nationallicence Fukuda Masaki Faculty of Agriculture, Shinshu University, 8304 Minamiminowa, 399-4598, Nagano, Japan aut Wakayama Masayoshi Faculty of Agriculture, Shinshu University, 8304 Minamiminowa, 399-4598, Nagano, Japan aut Uchida Masayuki Faculty of Agriculture, Shinshu University, 8304 Minamiminowa, 399-4598, Nagano, Japan aut Fukumasa-Nakai Yukitaka Tottori Mycological Institute, 689-1125, Tottori, Japan aut Matsumoto Teruyuki Tottori Mycological Institute, 689-1125, Tottori, Japan aut Journal of Wood Science Springer-Verlag 53/4(2007-08-01), 339-343 1435-0211 53:4<339 2007 53 10086 https://doi.org/10.1007/s10086-006-0861-9 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10086-006-0861-9 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Fukuda Masaki Faculty of Agriculture, Shinshu University, 8304 Minamiminowa, 399-4598, Nagano, Japan aut NATIONALLICENCE 700 1- Wakayama Masayoshi Faculty of Agriculture, Shinshu University, 8304 Minamiminowa, 399-4598, Nagano, Japan aut NATIONALLICENCE 700 1- Uchida Masayuki Faculty of Agriculture, Shinshu University, 8304 Minamiminowa, 399-4598, Nagano, Japan aut NATIONALLICENCE 700 1- Fukumasa-Nakai Yukitaka Tottori Mycological Institute, 689-1125, Tottori, Japan aut NATIONALLICENCE 700 1- Matsumoto Teruyuki Tottori Mycological Institute, 689-1125, Tottori, Japan aut NATIONALLICENCE 773 0- Journal of Wood Science Springer-Verlag 53/4(2007-08-01), 339-343 1435-0211 53:4<339 2007 53 10086 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer