caa a22 4500 463247620 CHVBK 20180405153330.0 cr unu---uuuuu 170326e20070801xx s 000 0 eng 10.1007/s10086-006-0859-3 doi (NATIONALLICENCE)springer-10.1007/s10086-006-0859-3 Analyses of the genetic diversity of matsutake isolates collected from different ecological environments in Asia [Elektronische Daten] [Dapeng Bao, Ayumi Koike, Fangjie Yao, Katsuji Yamanaka, Tadanori Aimi, Yutaka Kitamoto] Matsutake mushrooms are able to grow in two kinds of ecological environments: coniferous forests and broadleaf forests. Japanese matsutake is a form of the matsutake mushroom that mainly appears in coniferous forests in the mid-latitude regions of Asia. In contrast, matsutake from Yunnan and Sichuan Provinces of China grows in broadleaf forests in low-latitude regions. The taxonomic relationship and the genetic distance between these two types of matsutake remain unclear. Here, we compared the random amplification of polymorphic DNA (RAPD) data and the sequences of the internal transcribed spacer (ITS) region and of variable domains V4, V6, and V9 of mitochondrial small subunit ribosomal DNA (mt SSU rDNA) to determine the genetic differences of Tricholoma matsutake strains collected from different ecological and geographic environments in Asia. We found few differences in the sequences of the variable domains, implying that all matsutake strains are the same biological species regardless of their host specificity and geographical differences. This conclusion is also supported by the sequences of the ITS region. On the other hand, RAPD analysis revealed that the matsutake mushrooms collected from different ecological environments have genetic differences and represent distinct varieties. The Japan Wood Research Society, 2007 Matsutake nationallicence Tricholoma matsutake nationallicence Genetic diversity nationallicence Ecological environment nationallicence Bao Dapeng Laboratory of Microbial Biotechnology, Faculty of Agriculture, Tottori University, 680-8553, Tottori, Japan aut Koike Ayumi Laboratory of Microbial Biotechnology, Faculty of Agriculture, Tottori University, 680-8553, Tottori, Japan aut Yao Fangjie Institute of Mycology, Jilin Agricultural University, 130118, Changchun City, Jilin Province, P. R. China aut Yamanaka Katsuji Kyoto Mycological Institute, Misasahirabayashi, Yamashina, 607-8406, Kyoto, Japan aut Aimi Tadanori Laboratory of Microbial Biotechnology, Faculty of Agriculture, Tottori University, 680-8553, Tottori, Japan aut Kitamoto Yutaka Asano Life Science Laboratory, Asano Industry Co. Ltd., 3-20-6 Tamahara, 706-0014, Tamano, Japan aut Journal of Wood Science Springer-Verlag 53/4(2007-08-01), 344-350 1435-0211 53:4<344 2007 53 10086 https://doi.org/10.1007/s10086-006-0859-3 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10086-006-0859-3 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Bao Dapeng Laboratory of Microbial Biotechnology, Faculty of Agriculture, Tottori University, 680-8553, Tottori, Japan aut NATIONALLICENCE 700 1- Koike Ayumi Laboratory of Microbial Biotechnology, Faculty of Agriculture, Tottori University, 680-8553, Tottori, Japan aut NATIONALLICENCE 700 1- Yao Fangjie Institute of Mycology, Jilin Agricultural University, 130118, Changchun City, Jilin Province, P. R. China aut NATIONALLICENCE 700 1- Yamanaka Katsuji Kyoto Mycological Institute, Misasahirabayashi, Yamashina, 607-8406, Kyoto, Japan aut NATIONALLICENCE 700 1- Aimi Tadanori Laboratory of Microbial Biotechnology, Faculty of Agriculture, Tottori University, 680-8553, Tottori, Japan aut NATIONALLICENCE 700 1- Kitamoto Yutaka Asano Life Science Laboratory, Asano Industry Co. Ltd., 3-20-6 Tamahara, 706-0014, Tamano, Japan aut NATIONALLICENCE 773 0- Journal of Wood Science Springer-Verlag 53/4(2007-08-01), 344-350 1435-0211 53:4<344 2007 53 10086 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer