caa a22 4500 463247639 CHVBK 20180405153330.0 cr unu---uuuuu 170326e20070801xx s 000 0 eng 10.1007/s10086-006-0862-8 doi (NATIONALLICENCE)springer-10.1007/s10086-006-0862-8 Hafrén Jonas Department of Wood Science, Swedish University of Agricultural Sciences, Wood Ultrastructure Research Centre (WURC), Box 7008, SE-750 07, Uppsala, Sweden aut Excitation wavelength-specific changes in lignocellulosic autofluorescence [Elektronische Daten] [Jonas Hafrén] Lignocellulose (paper sheets of thermomechanical pulp) was irradiated with specific wavelengths in an epifluorescence microscope equipped with filter cubes with excitation and emission filters commonly used for excitation of fluorophores with blue, green, and red emissions. The irradiation was shown to affect the relative autofluorescence intensity over time. Shorter wavelength excitation (λex = 340-380 nm) induced photoyellowing that increased the autofluorescence. Filter cubes allowing irradiation at longer wavelengths (λex = 450-490 nm and λex = 515-560, respectively) caused photobleaching and a decrease in lignocellulosic autofluorescence. The increase and decrease in autofluorescence was reversible; prephotoyellowed samples could be photobleached and prephotobleached samples regained autofluorescence by shorter wavelength irradiation. Thus, the specificity of the excitation wavelength and the time-dependent autofluorescence might affect long-term imaging experiments of lignocellulose-based samples. The Japan Wood Research Society, 2007 Autofluorescence nationallicence Lignocellulose nationallicence Photobleaching nationallicence Photoyellowing nationallicence Thermomechanical pulp nationallicence Journal of Wood Science Springer-Verlag 53/4(2007-08-01), 358-360 1435-0211 53:4<358 2007 53 10086 https://doi.org/10.1007/s10086-006-0862-8 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10086-006-0862-8 text/html Onlinezugriff via DOI NATIONALLICENCE 100 1- Hafrén Jonas Department of Wood Science, Swedish University of Agricultural Sciences, Wood Ultrastructure Research Centre (WURC), Box 7008, SE-750 07, Uppsala, Sweden aut NATIONALLICENCE 773 0- Journal of Wood Science Springer-Verlag 53/4(2007-08-01), 358-360 1435-0211 53:4<358 2007 53 10086 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer