caa a22 4500 463247752 CHVBK 20180405153331.0 cr unu---uuuuu 170326e20071001xx s 000 0 eng 10.1007/s10086-007-0888-6 doi (NATIONALLICENCE)springer-10.1007/s10086-007-0888-6 Transcriptional and translational analyses of poplar anionic peroxidase isoenzymes [Elektronische Daten] [Shinya Sasaki, Motoyuki Shimizu, Hiroyuki Wariishi, Yuji Tsutsumi, Ryuichiro Kondo] Anionic peroxidases have been proposed to be a key enzyme for lignification in poplar. On the other hand, there are many genes encoding an anionic peroxidase in Populus trichocarpa genome, and their physiological functions are still unknown. Ampholine isoelectric focusing analysis revealed anionic peroxidases as dominant peroxidases in enzyme preparations from various organs. Using two-dimensional electrophoresis (2-DE) followed by peptide mass fingerprint (PMF) analysis, we surveyed the localization of anionic peroxidase isoenzymes in various organs of Populus alba L. Peroxidase isoenzymes were extracted from various organs and fractionated by a Concanavallin A Sepharose column. Each protein was separated by 2-DE gels and some anionic peroxidase isoenzymes in each organ were identified via PMF analysis. Transcript and protein of individual peroxidase indicate that the expression profile of each isoenzyme is quite different, for example, organspecific gene, stress-response gene, and multifunction gene, even though they are in the same cluster. These results suggest that individual anionic isoenzymes in this small cluster were differently regulated at transcription, translation, or posttranslation. The Japan Wood Research Society, 2007 Anionic peroxidase nationallicence Lignification nationallicence Poplar nationallicence Real-time PCR nationallicence Two-dimensional gel electrophoresis nationallicence Sasaki Shinya Department of Forest and Forest Products Sciences, Kyushu University, 6-10-1 Hakozaki, Higashi-ku, 812-8581, Fukuoka, Japan aut Shimizu Motoyuki Department of Forest and Forest Products Sciences, Kyushu University, 6-10-1 Hakozaki, Higashi-ku, 812-8581, Fukuoka, Japan aut Wariishi Hiroyuki Department of Forest and Forest Products Sciences, Kyushu University, 6-10-1 Hakozaki, Higashi-ku, 812-8581, Fukuoka, Japan aut Tsutsumi Yuji Department of Forest and Forest Products Sciences, Kyushu University, 6-10-1 Hakozaki, Higashi-ku, 812-8581, Fukuoka, Japan aut Kondo Ryuichiro Department of Forest and Forest Products Sciences, Kyushu University, 6-10-1 Hakozaki, Higashi-ku, 812-8581, Fukuoka, Japan aut Journal of Wood Science Springer-Verlag 53/5(2007-10-01), 427-435 1435-0211 53:5<427 2007 53 10086 https://doi.org/10.1007/s10086-007-0888-6 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10086-007-0888-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Sasaki Shinya Department of Forest and Forest Products Sciences, Kyushu University, 6-10-1 Hakozaki, Higashi-ku, 812-8581, Fukuoka, Japan aut NATIONALLICENCE 700 1- Shimizu Motoyuki Department of Forest and Forest Products Sciences, Kyushu University, 6-10-1 Hakozaki, Higashi-ku, 812-8581, Fukuoka, Japan aut NATIONALLICENCE 700 1- Wariishi Hiroyuki Department of Forest and Forest Products Sciences, Kyushu University, 6-10-1 Hakozaki, Higashi-ku, 812-8581, Fukuoka, Japan aut NATIONALLICENCE 700 1- Tsutsumi Yuji Department of Forest and Forest Products Sciences, Kyushu University, 6-10-1 Hakozaki, Higashi-ku, 812-8581, Fukuoka, Japan aut NATIONALLICENCE 700 1- Kondo Ryuichiro Department of Forest and Forest Products Sciences, Kyushu University, 6-10-1 Hakozaki, Higashi-ku, 812-8581, Fukuoka, Japan aut NATIONALLICENCE 773 0- Journal of Wood Science Springer-Verlag 53/5(2007-10-01), 427-435 1435-0211 53:5<427 2007 53 10086 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer