caa a22 4500 46324799X CHVBK 20180405153331.0 cr unu---uuuuu 170326e20070401xx s 000 0 eng 10.1007/s10086-006-0824-1 doi (NATIONALLICENCE)springer-10.1007/s10086-006-0824-1 Screening of extracts of Japanese woods for melanin biosynthesis inhibition [Elektronische Daten] [Kuniyoshi Shimizu, Shoko Fukunaga, Keisuke Yoshikawa, Ryuichiro Kondo] The effects of 120 methanol extracts prepared from bark and heartwood of 69 types of Japanese wood on the melanin production of B16 melanoma cells were examined. The melanin content of B16 melanoma cells was determined spectrophotometrically at 405nm. The extracts were also examined for their effects on cell viability. We found that the methanol extracts of Fagus crenata (buna, wood, 100μg/ml), Sapium sebiferum (Nankinhaze, wood, bark, 10μg/ml), and Zelkova serrata (keyaki, wood, 10μg/ml) greatly inhibited the melanin production of B16 melanoma cells without significant cytotoxicity. However, these extracts did not inhibit tyrosinase activity at the concentration of 100μg/ml. These findings indicate that the depigmenting mechanism of these extracts involves the suppression of some pigmenting signals in stimulating melanogenesis rather than the inhibition of tyrosinase activity. The Japan Wood Research Society, 2006 Melanin biosynthesis inhibitor nationallicence Fagus crenata nationallicence Sapium sebiferum nationallicence Zelkova serrata nationallicence Tyrosinase nationallicence Shimizu Kuniyoshi Department of Forest and Forest Products Sciences, Faculty of Agriculture, Kyushu University, 6-10-1 Hakozaki, 812-8581, Higashi-ku, Fukuoka, Japan aut Fukunaga Shoko Department of Forest and Forest Products Sciences, Faculty of Agriculture, Kyushu University, 6-10-1 Hakozaki, 812-8581, Higashi-ku, Fukuoka, Japan aut Yoshikawa Keisuke Department of Forest and Forest Products Sciences, Faculty of Agriculture, Kyushu University, 6-10-1 Hakozaki, 812-8581, Higashi-ku, Fukuoka, Japan aut Kondo Ryuichiro Department of Forest and Forest Products Sciences, Faculty of Agriculture, Kyushu University, 6-10-1 Hakozaki, 812-8581, Higashi-ku, Fukuoka, Japan aut Journal of Wood Science Springer-Verlag 53/2(2007-04-01), 153-160 1435-0211 53:2<153 2007 53 10086 https://doi.org/10.1007/s10086-006-0824-1 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10086-006-0824-1 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Shimizu Kuniyoshi Department of Forest and Forest Products Sciences, Faculty of Agriculture, Kyushu University, 6-10-1 Hakozaki, 812-8581, Higashi-ku, Fukuoka, Japan aut NATIONALLICENCE 700 1- Fukunaga Shoko Department of Forest and Forest Products Sciences, Faculty of Agriculture, Kyushu University, 6-10-1 Hakozaki, 812-8581, Higashi-ku, Fukuoka, Japan aut NATIONALLICENCE 700 1- Yoshikawa Keisuke Department of Forest and Forest Products Sciences, Faculty of Agriculture, Kyushu University, 6-10-1 Hakozaki, 812-8581, Higashi-ku, Fukuoka, Japan aut NATIONALLICENCE 700 1- Kondo Ryuichiro Department of Forest and Forest Products Sciences, Faculty of Agriculture, Kyushu University, 6-10-1 Hakozaki, 812-8581, Higashi-ku, Fukuoka, Japan aut NATIONALLICENCE 773 0- Journal of Wood Science Springer-Verlag 53/2(2007-04-01), 153-160 1435-0211 53:2<153 2007 53 10086 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer