caa a22 4500 463249194 CHVBK 20180405153334.0 cr unu---uuuuu 170326e20071201xx s 000 0 eng 10.1007/s11130-007-0060-x doi (NATIONALLICENCE)springer-10.1007/s11130-007-0060-x Towards Generating Caffeine-free Tea by Metabolic Engineering [Elektronische Daten] [Sudesh Yadav, Paramvir Ahuja] Tea is a rich source of antioxidants which are contributing substantially to the promotion of health and the prevention of various chronic diseases. Despite the fact that tea has various important compounds, it also contains a purine alkaloid, caffeine. High intake of tea leads to an increase in level of caffeine in addition to its important antioxidant constituents. Increased level of caffeine causes several health related problems. Therefore, tea can become a most useful source of beneficial compounds, if only its caffeine level is either decreased or eliminated all together from the plant itself. This could be achieved through either of the techniques; overexpressing caffeine degradative pathway genes or silencing caffeine biosynthesis pathway gene. The identification and cloning of caffeine biosynthesis in tea and degradative genes in microorganisms opens up the possibility of using genetic engineering to produce naturally decaffeinated tea. Here we review these different strategies which can be employed to make caffeine-free tea, a human health beneficial drink. Springer Science+Business Media, LLC, 2007 Antisense nationallicence Caffeine nationallicence Metabolic engineering nationallicence RNA interference nationallicence Tea nationallicence Yadav Sudesh Biotechnology Division, Institute of Himalayan Bioresource Technology, CSIR, 176061, Palampur, H.P., India aut Ahuja Paramvir Biotechnology Division, Institute of Himalayan Bioresource Technology, CSIR, 176061, Palampur, H.P., India aut Plant Foods for Human Nutrition Springer US; http://www.springer-ny.com 62/4(2007-12-01), 185-191 0921-9668 62:4<185 2007 62 11130 https://doi.org/10.1007/s11130-007-0060-x text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11130-007-0060-x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Yadav Sudesh Biotechnology Division, Institute of Himalayan Bioresource Technology, CSIR, 176061, Palampur, H.P., India aut NATIONALLICENCE 700 1- Ahuja Paramvir Biotechnology Division, Institute of Himalayan Bioresource Technology, CSIR, 176061, Palampur, H.P., India aut NATIONALLICENCE 773 0- Plant Foods for Human Nutrition Springer US; http://www.springer-ny.com 62/4(2007-12-01), 185-191 0921-9668 62:4<185 2007 62 11130 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer