caa a22 4500 463254058 CHVBK 20180405153349.0 cr unu---uuuuu 170326e20070101xx s 000 0 eng 10.1007/s10571-006-9115-z doi (NATIONALLICENCE)springer-10.1007/s10571-006-9115-z Harthoorn Lucien Netherlands Institute for Brain Research, Amsterdam, The Netherlands aut Projection-Dependent Differentiation of Melanin-Concentrating Hormone-Containing Neurons [Elektronische Daten] [Lucien Harthoorn] Summary: 1. Melanin-concentrating hormone (MCH)-containing neurons within the lateral hypothalamus affect a broad spectrum of physiological and behavioral processes. The aim of this study was to examine a differentiation of rat MCH neurons dependent on their functionally related projections to the intermediolateral column (IML) of the spinal cord, the midbrain periaqueductal gray (PAG), and the brainstem locus coeruleus (LC). 2. A combination of retrograde fluorescent neuronal tracing and immunocytochemical identification revealed MCH neurons that project to IML, PAG, and LC. Subsequent to isolation of these single neurons by laser microdissection, multi-transcriptional profiling by means of real-time RT-PCR demonstrated different expression patterns between sympathetically projecting MCH neurons and those targeting the PAG or LC. 3. These results provide evidence for the existence of projection-dependent MCH neuronal subpopulations with functional differentiation in the context of controlling energy homeostasis, autonomic functioning, and arousal. Springer Science+Business Media, Inc., 2006 gene expression nationallicence hypothalamus nationallicence food intake nationallicence wakefulness nationallicence rat nationallicence Cellular and Molecular Neurobiology Springer US; http://www.springer-ny.com 27/1(2007-01-01), 49-55 0272-4340 27:1<49 2007 27 10571 https://doi.org/10.1007/s10571-006-9115-z text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10571-006-9115-z text/html Onlinezugriff via DOI NATIONALLICENCE 100 1- Harthoorn Lucien Netherlands Institute for Brain Research, Amsterdam, The Netherlands aut NATIONALLICENCE 773 0- Cellular and Molecular Neurobiology Springer US; http://www.springer-ny.com 27/1(2007-01-01), 49-55 0272-4340 27:1<49 2007 27 10571 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer