caa a22 4500 463254147 CHVBK 20180405153349.0 cr unu---uuuuu 170326e20070901xx s 000 0 eng 10.1007/s10571-007-9171-z doi (NATIONALLICENCE)springer-10.1007/s10571-007-9171-z A Calcium-Receptor Agonist Induces Gustatory Neural Responses in Bullfrogs [Elektronische Daten] [Yukio Okada, Kotapola Imendra, Toshihiro Miyazaki, Hitoshi Hotokezaka, Rie Fujiyama, Jorge Zeredo, Kazuo Toda] The effect of calcium-sensing receptor (CaR) agonists on frog gustatory responses was studied using glossopharyngeal nerve recording and whole-cell patch-clamp recording of isolated taste disc cells. Calcimimetic NPS R-467 dissolved in normal saline solution elicited a large transient response in the nerve. The less active enantiomer of the compound, NPS S-467 induced only a small neural response. The EC50 for NPS R-467 was about 20μM. Cross-adaptation experiments were performed to examine the effect of 30μM NPS R-467 and 100μM quinine on the gustatory neural response. The magnitude of the R-467-induced response after adaptation to quinine was approximately equal to that after adaptation to normal saline solution, indicating that the receptor site for NPS R-467 is different from the site for quinine. NPS R-467 (100μM) also induced an inward current accompanied with conductance increase and large depolarization in two (13%) of 15 rod cells, and a sustained decrease in outward current and small depolarization in six (40%) other rod cells. NPS S-467 (100μM) induced a sustained decrease in outward current and depolarization in five (50%) of 10 rod cells. Another calcimimetic cinacalcet (100μM) induced an inward current accompanied with conductance increase in three (27%) of 11 rod cells. The results suggest that NPS R-467 induces neural responses through cell responses unrelated to a resting K+ conductance decrease. Springer Science+Business Media, LLC, 2007 Rana nationallicence Gustatory neural response nationallicence Ca2+-sensing receptor nationallicence Calcimimetics nationallicence Patch-clamp nationallicence Taste disc cell nationallicence Okada Yukio Integrative Sensory Physiology, Graduate School of Biomedical Sciences, Nagasaki University, 1-7-1 Sakamoto, 852-8588, Nagasaki, Nagasaki, Japan aut Imendra Kotapola Department of Physiology, Faculty of Medicine, University of Ruhuna, Galle, Sri Lanka aut Miyazaki Toshihiro Cell Biology, Graduate School of Biomedical Sciences, Nagasaki University, 852-8588, Nagasaki, Nagasaki, Japan aut Hotokezaka Hitoshi Orthodontics and Biomedical Engineering, Graduate School of Biomedical Sciences, Nagasaki University, 852-8588, Nagasaki, Nagasaki, Japan aut Fujiyama Rie Integrative Sensory Physiology, Graduate School of Biomedical Sciences, Nagasaki University, 1-7-1 Sakamoto, 852-8588, Nagasaki, Nagasaki, Japan aut Zeredo Jorge Integrative Sensory Physiology, Graduate School of Biomedical Sciences, Nagasaki University, 1-7-1 Sakamoto, 852-8588, Nagasaki, Nagasaki, Japan aut Toda Kazuo Integrative Sensory Physiology, Graduate School of Biomedical Sciences, Nagasaki University, 1-7-1 Sakamoto, 852-8588, Nagasaki, Nagasaki, Japan aut Cellular and Molecular Neurobiology Springer US; http://www.springer-ny.com 27/6(2007-09-01), 771-781 0272-4340 27:6<771 2007 27 10571 https://doi.org/10.1007/s10571-007-9171-z text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10571-007-9171-z text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Okada Yukio Integrative Sensory Physiology, Graduate School of Biomedical Sciences, Nagasaki University, 1-7-1 Sakamoto, 852-8588, Nagasaki, Nagasaki, Japan aut NATIONALLICENCE 700 1- Imendra Kotapola Department of Physiology, Faculty of Medicine, University of Ruhuna, Galle, Sri Lanka aut NATIONALLICENCE 700 1- Miyazaki Toshihiro Cell Biology, Graduate School of Biomedical Sciences, Nagasaki University, 852-8588, Nagasaki, Nagasaki, Japan aut NATIONALLICENCE 700 1- Hotokezaka Hitoshi Orthodontics and Biomedical Engineering, Graduate School of Biomedical Sciences, Nagasaki University, 852-8588, Nagasaki, Nagasaki, Japan aut NATIONALLICENCE 700 1- Fujiyama Rie Integrative Sensory Physiology, Graduate School of Biomedical Sciences, Nagasaki University, 1-7-1 Sakamoto, 852-8588, Nagasaki, Nagasaki, Japan aut NATIONALLICENCE 700 1- Zeredo Jorge Integrative Sensory Physiology, Graduate School of Biomedical Sciences, Nagasaki University, 1-7-1 Sakamoto, 852-8588, Nagasaki, Nagasaki, Japan aut NATIONALLICENCE 700 1- Toda Kazuo Integrative Sensory Physiology, Graduate School of Biomedical Sciences, Nagasaki University, 1-7-1 Sakamoto, 852-8588, Nagasaki, Nagasaki, Japan aut NATIONALLICENCE 773 0- Cellular and Molecular Neurobiology Springer US; http://www.springer-ny.com 27/6(2007-09-01), 771-781 0272-4340 27:6<771 2007 27 10571 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer