caa a22 4500 463254171 CHVBK 20180405153349.0 cr unu---uuuuu 170326e20070901xx s 000 0 eng 10.1007/s10571-007-9160-2 doi (NATIONALLICENCE)springer-10.1007/s10571-007-9160-2 Investigation on Mitochondrial tRNALeu/Lys, NDI and ATPase 6/8 in Iranian Multiple Sclerosis Patients [Elektronische Daten] [Solmaz Ahari, Massoud Houshmand, Mehdi Panahi, Sadaf Kasraie, Mostafa Moin, Mohammad Bahar] As with chromosomal DNA, the mitochondrial DNA (mtDNA) can contain mutations that are highly pathogenic .In fact, many diseases of the central nervous system are known to be caused by mutations in mtDNA. Dysfunction of the mitochondrial Respiratory Chain (RC) has been shown in patients with neurological disease including Alzheimer's disease (AD), Parkinson's disease (PD) and Multiple sclerosis (MS). MS is a demyelinating disease of central nervous system characterized by morphological hallmarks of inflammation, demyelination and axonal loss. Considering this importance, we decided to investigate several highly mutative parts of mtDNA for point mutations as MT-LTI (tRNALeucine1(UUA/G)), MT-NDI (NADH Dehydrogenase subunit 1), MT-COII (Cytochrome c oxidase subunit II), MT-TK (tRNALysine), MT-ATP8 (ATP synthase subunit F0 8) and MT-ATP6 (ATP synthase subunit F0 6) in 20 Iranian MS patients and 80 age-matched control subjects by PCR and automated DNA sequencing to evaluate any probable point mutations. Our results revealed that 15 (75%) out of 20MS patients had point mutations. Some of point mutations were newly found in this study. This study suggested that point mutation occurred in mtDNA might be involved in pathogenesis of MS. Springer Science+Business Media, LLC, 2007 Mitochondrial DNA nationallicence Multiple Sclerosis nationallicence Ahari Solmaz Sciences & Research Campus, Islamic Azad University, Tehran, Iran aut Houshmand Massoud Department of Medical Genetics, National Institute of Genetic Engineering and Biotechnology (NIGEB), Pajoohesh Blvd, Tehran-Karaj Highway, 17th km, P.O. Box 14155-6343, Tehran, Iran aut Panahi Mehdi Department of Medical Genetics, National Institute of Genetic Engineering and Biotechnology (NIGEB), Pajoohesh Blvd, Tehran-Karaj Highway, 17th km, P.O. Box 14155-6343, Tehran, Iran aut Kasraie Sadaf Sciences & Research Campus, Islamic Azad University, Tehran, Iran aut Moin Mostafa Immunology, Asthma & Allergy Research Institute, Tehran, Iran aut Bahar Mohammad Shahid Motahari Burn & Reconstruction Research Center, Iran University of Medical Sciences & Health Services, Tehran, Iran aut Cellular and Molecular Neurobiology Springer US; http://www.springer-ny.com 27/6(2007-09-01), 695-700 0272-4340 27:6<695 2007 27 10571 https://doi.org/10.1007/s10571-007-9160-2 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10571-007-9160-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Ahari Solmaz Sciences & Research Campus, Islamic Azad University, Tehran, Iran aut NATIONALLICENCE 700 1- Houshmand Massoud Department of Medical Genetics, National Institute of Genetic Engineering and Biotechnology (NIGEB), Pajoohesh Blvd, Tehran-Karaj Highway, 17th km, P.O. Box 14155-6343, Tehran, Iran aut NATIONALLICENCE 700 1- Panahi Mehdi Department of Medical Genetics, National Institute of Genetic Engineering and Biotechnology (NIGEB), Pajoohesh Blvd, Tehran-Karaj Highway, 17th km, P.O. Box 14155-6343, Tehran, Iran aut NATIONALLICENCE 700 1- Kasraie Sadaf Sciences & Research Campus, Islamic Azad University, Tehran, Iran aut NATIONALLICENCE 700 1- Moin Mostafa Immunology, Asthma & Allergy Research Institute, Tehran, Iran aut NATIONALLICENCE 700 1- Bahar Mohammad Shahid Motahari Burn & Reconstruction Research Center, Iran University of Medical Sciences & Health Services, Tehran, Iran aut NATIONALLICENCE 773 0- Cellular and Molecular Neurobiology Springer US; http://www.springer-ny.com 27/6(2007-09-01), 695-700 0272-4340 27:6<695 2007 27 10571 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer