caa a22 4500 46325421X CHVBK 20180405153349.0 cr unu---uuuuu 170326e20070901xx s 000 0 eng 10.1007/s10571-007-9195-4 doi (NATIONALLICENCE)springer-10.1007/s10571-007-9195-4 Pericytes from Brain Microvessels Strengthen the Barrier Integrity in Primary Cultures of Rat Brain Endothelial Cells [Elektronische Daten] [Shinsuke Nakagawa, Mária Deli, Shinobu Nakao, Masaru Honda, Kentaro Hayashi, Ryota Nakaoke, Yasufumi Kataoka, Masami Niwa] (1) The blood-brain barrier (BBB) characteristics of cerebral endothelial cells are induced by organ-specific local signals. Brain endothelial cells lose their phenotype in cultures without cross-talk with neighboring cells. (2) In contrast to astrocytes, pericytes, another neighboring cell of endothelial cells in brain capillaries, are rarely used in BBB co-culture systems. (3) Seven different types of BBB models, mono-culture, double and triple co-cultures, were constructed from primary rat brain endothelial cells, astrocytes and pericytes on culture inserts. The barrier integrity of the models were compared by measurement of transendothelial electrical resistance and permeability for the small molecular weight marker fluorescein. (4) We could confirm that brain endothelial monolayers in mono-culture do not form tight barrier. Pericytes induced higher electrical resistance and lower permeability for fluorescein than type I astrocytes in co-culture conditions. In triple co-culture models the tightest barrier was observed when endothelial cells and pericytes were positioned on the two sides of the porous filter membrane of the inserts and astrocytes at the bottom of the culture dish. (5) For the first time a rat primary culture based syngeneic triple co-culture BBB model has been constructed using brain pericytes beside brain endothelial cells and astrocytes. This model, mimicking closely the anatomical position of the cells at the BBB invivo, was superior to the other BBB models tested. (6) The influence of pericytes on the BBB properties of brain endothelial cells may be as important as that of astrocytes and could be exploited in the construction of better BBB models. The Author(s), 2007 Pericytes nationallicence Blood-brain barrier nationallicence Brain endothelial cells nationallicence Astrocytes nationallicence Co-culture nationallicence Invitro BBB model (rat) nationallicence Permeability nationallicence Transendothelial electrical resistance nationallicence Nakagawa Shinsuke Department of Pharmacology, Nagasaki University Graduate School of Biomedical Sciences, 1-12-4 Sakamoto, 852-8523, Nagasaki, Japan aut Deli Mária Department of Pharmacology, Nagasaki University Graduate School of Biomedical Sciences, 1-12-4 Sakamoto, 852-8523, Nagasaki, Japan aut Nakao Shinobu Department of Pharmacology, Nagasaki University Graduate School of Biomedical Sciences, 1-12-4 Sakamoto, 852-8523, Nagasaki, Japan aut Honda Masaru Department of Neurosurgery, Nagasaki University Graduate School of Biomedical Sciences, 1-12-4 Sakamoto, 852-8523, Nagasaki, Japan aut Hayashi Kentaro Department of Neurosurgery, Nagasaki University Graduate School of Biomedical Sciences, 1-12-4 Sakamoto, 852-8523, Nagasaki, Japan aut Nakaoke Ryota Department of Pharmacology, Nagasaki University Graduate School of Biomedical Sciences, 1-12-4 Sakamoto, 852-8523, Nagasaki, Japan aut Kataoka Yasufumi BBB Laboratory, PharmaCo-Cell Co. Ltd, 1-12-4 Sakamoto, 852-8523, Nagasaki, Japan aut Niwa Masami Department of Pharmacology, Nagasaki University Graduate School of Biomedical Sciences, 1-12-4 Sakamoto, 852-8523, Nagasaki, Japan aut Cellular and Molecular Neurobiology Springer US; http://www.springer-ny.com 27/6(2007-09-01), 687-694 0272-4340 27:6<687 2007 27 10571 https://doi.org/10.1007/s10571-007-9195-4 text/html Onlinezugriff via DOI 1 brief-communication jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10571-007-9195-4 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Nakagawa Shinsuke Department of Pharmacology, Nagasaki University Graduate School of Biomedical Sciences, 1-12-4 Sakamoto, 852-8523, Nagasaki, Japan aut NATIONALLICENCE 700 1- Deli Mária Department of Pharmacology, Nagasaki University Graduate School of Biomedical Sciences, 1-12-4 Sakamoto, 852-8523, Nagasaki, Japan aut NATIONALLICENCE 700 1- Nakao Shinobu Department of Pharmacology, Nagasaki University Graduate School of Biomedical Sciences, 1-12-4 Sakamoto, 852-8523, Nagasaki, Japan aut NATIONALLICENCE 700 1- Honda Masaru Department of Neurosurgery, Nagasaki University Graduate School of Biomedical Sciences, 1-12-4 Sakamoto, 852-8523, Nagasaki, Japan aut NATIONALLICENCE 700 1- Hayashi Kentaro Department of Neurosurgery, Nagasaki University Graduate School of Biomedical Sciences, 1-12-4 Sakamoto, 852-8523, Nagasaki, Japan aut NATIONALLICENCE 700 1- Nakaoke Ryota Department of Pharmacology, Nagasaki University Graduate School of Biomedical Sciences, 1-12-4 Sakamoto, 852-8523, Nagasaki, Japan aut NATIONALLICENCE 700 1- Kataoka Yasufumi BBB Laboratory, PharmaCo-Cell Co. Ltd, 1-12-4 Sakamoto, 852-8523, Nagasaki, Japan aut NATIONALLICENCE 700 1- Niwa Masami Department of Pharmacology, Nagasaki University Graduate School of Biomedical Sciences, 1-12-4 Sakamoto, 852-8523, Nagasaki, Japan aut NATIONALLICENCE 773 0- Cellular and Molecular Neurobiology Springer US; http://www.springer-ny.com 27/6(2007-09-01), 687-694 0272-4340 27:6<687 2007 27 10571 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer