caa a22 4500 463254236 CHVBK 20180405153349.0 cr unu---uuuuu 170326e20070901xx s 000 0 eng 10.1007/s10571-007-9148-y doi (NATIONALLICENCE)springer-10.1007/s10571-007-9148-y Axon Viability and Mitochondrial Function are Dependent on Local Protein Synthesis in Sympathetic Neurons [Elektronische Daten] [Mi Hillefors, Anthony Gioio, Marie Mameza, Barry Kaplan] (1) Axons contain numerous mRNAs and a local protein synthetic system that can be regulated independently of the cell body. (2) In this study, cultured primary sympathetic neurons were employed, to assess the effect of local protein synthesis blockade on axon viability and mitochondrial function. (3) Inhibition of local protein synthesis reduced newly synthesized axonal proteins by 65% and resulted in axon retraction after 6h. Acute inhibition of local protein synthesis also resulted in a significant decrease in the membrane potential of axonal mitochondria. Likewise, blockade of local protein transport into the mitochondria by transfection of the axons with Hsp90 C-terminal domain decreased the mitochondrial membrane potential by 65%. Moreover, inhibition of the local protein synthetic system also reduced the ability of mitochondria to restore axonal levels of ATP after KCl-induced depolarization. (4) Taken together, these results indicate that the local protein synthetic system plays an important role in mitochondrial function and the maintenance of the axon. Springer Science+Business Media, LLC, 2007 Superior cervical ganglia nationallicence Axon nationallicence Local protein synthesis nationallicence Mitochondrial membrane potential nationallicence ATP levels nationallicence Hillefors Mi Laboratory of Molecular Biology, National Institute of Mental Health, National Institutes of Health, Building 10, Room 4N222, 20892-1381, Bethesda, MD, USA aut Gioio Anthony Laboratory of Molecular Biology, National Institute of Mental Health, National Institutes of Health, Building 10, Room 4N222, 20892-1381, Bethesda, MD, USA aut Mameza Marie Laboratory of Molecular Biology, National Institute of Mental Health, National Institutes of Health, Building 10, Room 4N222, 20892-1381, Bethesda, MD, USA aut Kaplan Barry Laboratory of Molecular Biology, National Institute of Mental Health, National Institutes of Health, Building 10, Room 4N222, 20892-1381, Bethesda, MD, USA aut Cellular and Molecular Neurobiology Springer US; http://www.springer-ny.com 27/6(2007-09-01), 701-716 0272-4340 27:6<701 2007 27 10571 https://doi.org/10.1007/s10571-007-9148-y text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10571-007-9148-y text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Hillefors Mi Laboratory of Molecular Biology, National Institute of Mental Health, National Institutes of Health, Building 10, Room 4N222, 20892-1381, Bethesda, MD, USA aut NATIONALLICENCE 700 1- Gioio Anthony Laboratory of Molecular Biology, National Institute of Mental Health, National Institutes of Health, Building 10, Room 4N222, 20892-1381, Bethesda, MD, USA aut NATIONALLICENCE 700 1- Mameza Marie Laboratory of Molecular Biology, National Institute of Mental Health, National Institutes of Health, Building 10, Room 4N222, 20892-1381, Bethesda, MD, USA aut NATIONALLICENCE 700 1- Kaplan Barry Laboratory of Molecular Biology, National Institute of Mental Health, National Institutes of Health, Building 10, Room 4N222, 20892-1381, Bethesda, MD, USA aut NATIONALLICENCE 773 0- Cellular and Molecular Neurobiology Springer US; http://www.springer-ny.com 27/6(2007-09-01), 701-716 0272-4340 27:6<701 2007 27 10571 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer