caa a22 4500 463254252 CHVBK 20180405153349.0 cr unu---uuuuu 170326e20070901xx s 000 0 eng 10.1007/s10571-007-9205-6 doi (NATIONALLICENCE)springer-10.1007/s10571-007-9205-6 Immunohistochemical and Biochemical Analyses of LGI3 in Monkey Brain: LGI3 Accumulates in Aged Monkey Brains [Elektronische Daten] [Sachi Okabayashi, Nobuyuki Kimura] Leucine-rich glioma inactivated (LGI) 3 encodes a leucine-rich repeat protein. The precise function of LGI3, however, remains unknown. We have previously shown that amyloid-β peptide (Aβ) upregulates LGI3 and that Aβ and LGI3 colocalize on plasma membranes of cultured rat astrocytes. In the present study, we performed immunohistochemical and biochemical analyses of LGI3 using various aged monkey brains. Immunohistochemistry showed that LGI3 was present in almost all neural cells and mainly localized at plasma membranes and nuclei. In aged monkey brains, we found that LGI3 accumulated on or near the plasma membranes of neurons, and colocalized with endocytosis-associated proteins and lipid raft markers. Double immunohistochemistry also showed that LGI3 colocalized with Aβ in astrocytes of aged brains. Moreover, Western blot analyses revealed that LGI3 may be cleaved in brain. Additionally, in aged monkeys LGI3 accumulated in microsomal and nuclear brain fractions. Springer Science+Business Media, LLC, 2007 Aging nationallicence Endocytosis nationallicence Leucine-rich glioma inactivated 3 nationallicence Monkey brains nationallicence Okabayashi Sachi Laboratory of Disease Control, Tsukuba Primate Research Center, National Institute of Biomedical Innovation, 1-1 Hachimandai, 305-0843, Tsukuba-shi, Ibaraki, Japan aut Kimura Nobuyuki Laboratory of Disease Control, Tsukuba Primate Research Center, National Institute of Biomedical Innovation, 1-1 Hachimandai, 305-0843, Tsukuba-shi, Ibaraki, Japan aut Cellular and Molecular Neurobiology Springer US; http://www.springer-ny.com 27/6(2007-09-01), 819-830 0272-4340 27:6<819 2007 27 10571 https://doi.org/10.1007/s10571-007-9205-6 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10571-007-9205-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Okabayashi Sachi Laboratory of Disease Control, Tsukuba Primate Research Center, National Institute of Biomedical Innovation, 1-1 Hachimandai, 305-0843, Tsukuba-shi, Ibaraki, Japan aut NATIONALLICENCE 700 1- Kimura Nobuyuki Laboratory of Disease Control, Tsukuba Primate Research Center, National Institute of Biomedical Innovation, 1-1 Hachimandai, 305-0843, Tsukuba-shi, Ibaraki, Japan aut NATIONALLICENCE 773 0- Cellular and Molecular Neurobiology Springer US; http://www.springer-ny.com 27/6(2007-09-01), 819-830 0272-4340 27:6<819 2007 27 10571 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer