caa a22 4500 463254279 CHVBK 20180405153350.0 cr unu---uuuuu 170326e20071101xx s 000 0 eng 10.1007/s10571-007-9210-9 doi (NATIONALLICENCE)springer-10.1007/s10571-007-9210-9 γ1-Dependent Down-regulation of Recombinant Voltage-gated Ca2+ Channels [Elektronische Daten] [Alejandro Sandoval, Jyothi Arikkath, Eduardo Monjaraz, Kevin Campbell, Ricardo Felix] (1) Voltage-gated Ca2+ (CaV) channels are multi-subunit membrane complexes that allow depolarization-induced Ca2+ influx into cells. The skeletal muscle L-type CaV channels consist of an ion-conducting CaV1.1 subunit and auxiliary α2δ−1, β1 and γ1 subunits. This complex serves both as a CaV channel and as a voltage sensor for excitation-contraction coupling. (2) Though much is known about the mechanisms by which the α2δ−1 and β1 subunits regulate CaV channel function, there is far less information on the γ1 subunit. Previously, we characterized the interaction of γ1 with the other components of the skeletal CaV channel complex, and showed that heterologous expression of this auxiliary subunit decreases Ca2+ current density in myotubes from γ1 null mice. (3) In the current report, using Western blotting we show that the expression of the CaV1.1 protein is significantly lower when it is heterologously co-expressed with γ1. Consistent with this, patch-clamp recordings showed that transient transfection of γ1 drastically inhibited macroscopic currents through recombinant N-type (CaV2.2/α2δ−1/β3) channels expressed in HEK-293 cells. (4) These findings provide evidence that co-expression of the auxiliary γ1 subunit results in a decreased expression of the ion-conducting subunit, which may help to explain the reduction in Ca2+ current density following γ1 transfection. Springer Science+Business Media, LLC, 2007 Ca2 + channels nationallicence γ1 subunit nationallicence HEK-293 cells nationallicence Patch-clamp nationallicence Sandoval Alejandro Department of Cell Biology, Center for Research and Advanced Studies of the National Polytechnic Institute (Cinvestav-IPN), Avenida IPN 2508, Colonia Zacatenco, CP 07300, Mexico City, DF, Mexico aut Arikkath Jyothi Howard Hughes Medical Institute (HHMI); Departments of Molecular Physiology and Biophysics, Internal Medicine, and Neurology, University of Iowa Carver, College of Medicine, Iowa, USA aut Monjaraz Eduardo Laboratory of Neuroendocrinology, Institute of Physiology, University of Puebla, Puebla, Mexico aut Campbell Kevin Howard Hughes Medical Institute (HHMI); Departments of Molecular Physiology and Biophysics, Internal Medicine, and Neurology, University of Iowa Carver, College of Medicine, Iowa, USA aut Felix Ricardo Department of Cell Biology, Center for Research and Advanced Studies of the National Polytechnic Institute (Cinvestav-IPN), Avenida IPN 2508, Colonia Zacatenco, CP 07300, Mexico City, DF, Mexico aut Cellular and Molecular Neurobiology Springer US; http://www.springer-ny.com 27/7(2007-11-01), 901-908 0272-4340 27:7<901 2007 27 10571 https://doi.org/10.1007/s10571-007-9210-9 text/html Onlinezugriff via DOI 1 brief-communication jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10571-007-9210-9 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Sandoval Alejandro Department of Cell Biology, Center for Research and Advanced Studies of the National Polytechnic Institute (Cinvestav-IPN), Avenida IPN 2508, Colonia Zacatenco, CP 07300, Mexico City, DF, Mexico aut NATIONALLICENCE 700 1- Arikkath Jyothi Howard Hughes Medical Institute (HHMI); Departments of Molecular Physiology and Biophysics, Internal Medicine, and Neurology, University of Iowa Carver, College of Medicine, Iowa, USA aut NATIONALLICENCE 700 1- Monjaraz Eduardo Laboratory of Neuroendocrinology, Institute of Physiology, University of Puebla, Puebla, Mexico aut NATIONALLICENCE 700 1- Campbell Kevin Howard Hughes Medical Institute (HHMI); Departments of Molecular Physiology and Biophysics, Internal Medicine, and Neurology, University of Iowa Carver, College of Medicine, Iowa, USA aut NATIONALLICENCE 700 1- Felix Ricardo Department of Cell Biology, Center for Research and Advanced Studies of the National Polytechnic Institute (Cinvestav-IPN), Avenida IPN 2508, Colonia Zacatenco, CP 07300, Mexico City, DF, Mexico aut NATIONALLICENCE 773 0- Cellular and Molecular Neurobiology Springer US; http://www.springer-ny.com 27/7(2007-11-01), 901-908 0272-4340 27:7<901 2007 27 10571 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer