caa a22 4500 463254287 CHVBK 20180405153350.0 cr unu---uuuuu 170326e20071101xx s 000 0 eng 10.1007/s10571-007-9207-4 doi (NATIONALLICENCE)springer-10.1007/s10571-007-9207-4 No Association Between the LRRK2 G2019S Mutation and Alzheimer's disease in Italy [Elektronische Daten] [Andrea Tedde, Silvia Bagnoli, Elena Cellini, Benedetta Nacmias, Silvia Piacentini, Sandro Sorbi] Aims Investigation of the leucine-rich repeat kinase 2 (LRRK2) gene in late-onset Alzheimer's disease (AD) patients to screen for the G2019S mutation, which is common in Parkinson's cases. Methods High-resolution melting analysis (HRMA) was used to screen a large sample of patients. The target sequence was amplified by standard PCR in the presence of an intercalating fluorescent dye. Heterozygotes were easily identified because the heteroduplexes produced changed the shape of the melting curve. Results In accordance to previous studies, we did not detect the G2019S mutation in any of the 769 Italian AD patients under study. Conclusions HMRA allowed us to rapidly characterize a large number of samples for the LRRK2 G2019S mutation, which results as absent in a large AD data set. Springer Science+Business Media, LLC, 2007 Alzheimer's disease nationallicence Genetics nationallicence Mutations nationallicence LRRK2 nationallicence Tedde Andrea Neurogenetics Unit, Department of Neurological and Psychiatric Sciences, University of Florence, Viale Pieraccini 6, 50139, Florence, Italy aut Bagnoli Silvia Neurogenetics Unit, Department of Neurological and Psychiatric Sciences, University of Florence, Viale Pieraccini 6, 50139, Florence, Italy aut Cellini Elena Neurogenetics Unit, Department of Neurological and Psychiatric Sciences, University of Florence, Viale Pieraccini 6, 50139, Florence, Italy aut Nacmias Benedetta Neurogenetics Unit, Department of Neurological and Psychiatric Sciences, University of Florence, Viale Pieraccini 6, 50139, Florence, Italy aut Piacentini Silvia Neurogenetics Unit, Department of Neurological and Psychiatric Sciences, University of Florence, Viale Pieraccini 6, 50139, Florence, Italy aut Sorbi Sandro Neurogenetics Unit, Department of Neurological and Psychiatric Sciences, University of Florence, Viale Pieraccini 6, 50139, Florence, Italy aut Cellular and Molecular Neurobiology Springer US; http://www.springer-ny.com 27/7(2007-11-01), 877-881 0272-4340 27:7<877 2007 27 10571 https://doi.org/10.1007/s10571-007-9207-4 text/html Onlinezugriff via DOI 1 brief-communication jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10571-007-9207-4 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Tedde Andrea Neurogenetics Unit, Department of Neurological and Psychiatric Sciences, University of Florence, Viale Pieraccini 6, 50139, Florence, Italy aut NATIONALLICENCE 700 1- Bagnoli Silvia Neurogenetics Unit, Department of Neurological and Psychiatric Sciences, University of Florence, Viale Pieraccini 6, 50139, Florence, Italy aut NATIONALLICENCE 700 1- Cellini Elena Neurogenetics Unit, Department of Neurological and Psychiatric Sciences, University of Florence, Viale Pieraccini 6, 50139, Florence, Italy aut NATIONALLICENCE 700 1- Nacmias Benedetta Neurogenetics Unit, Department of Neurological and Psychiatric Sciences, University of Florence, Viale Pieraccini 6, 50139, Florence, Italy aut NATIONALLICENCE 700 1- Piacentini Silvia Neurogenetics Unit, Department of Neurological and Psychiatric Sciences, University of Florence, Viale Pieraccini 6, 50139, Florence, Italy aut NATIONALLICENCE 700 1- Sorbi Sandro Neurogenetics Unit, Department of Neurological and Psychiatric Sciences, University of Florence, Viale Pieraccini 6, 50139, Florence, Italy aut NATIONALLICENCE 773 0- Cellular and Molecular Neurobiology Springer US; http://www.springer-ny.com 27/7(2007-11-01), 877-881 0272-4340 27:7<877 2007 27 10571 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer