caa a22 4500 463254309 CHVBK 20180405153350.0 cr unu---uuuuu 170326e20071101xx s 000 0 eng 10.1007/s10571-007-9222-5 doi (NATIONALLICENCE)springer-10.1007/s10571-007-9222-5 Interaction of Delta Sleep-inducing Peptide and Valproate on Metaphit Audiogenic Seizure Model in Rats [Elektronische Daten] [Olivera Stanojlović, Dragan Hrnčić, Aleksandra Rašić, Helena Lončar-Stevanović, Dragan Djuric, Veselinka Šušić] Effects of valproate (VPA), a conventional antiepileptic drug and natural delta sleep-inducing peptide (DSIP) on metaphit (1-[1-(3-isothiocyanatophenyl)-cyclohexyl]-piperidine)-induced audiogenic reflex epilepsy were studied. For the purpose of the study, valproate in the doses of 50 or 75mg/kg and DSIP (1.0mg/kg) was i.p. injected either alone or in combination to adult Wistar male rats with fully developed metaphit seizures after eight audiogenic testing. The animals were stimulated using an electric bell (100±3dB and 5-8kHz, for 60s) 60min after metaphit injection and afterwards at hourly intervals during the experiment. For EEG recording and power spectra analysis, three gold-plated screws were implanted into the scull. In EEGs of metaphit-treated animals polyspikes, spike-wave complexes and sleep-like patterns were recorded, while the power spectra were increased. Combined treatment of metaphit-induced seizures with valproate and DSIP was more effective than drugs alone especially during 4h after administration. None of the applied dose combinations eliminated the EEG signs of metaphit-provoked epileptiform activity. Taken together, the results of the present study suggest that the combinations of valproate and DSIP should be considered as beneficial polytherapy in metaphit model of epilepsy. Springer Science+Business Media, LLC, 2007 Audiogenic seizures nationallicence DSIP nationallicence Metaphit nationallicence Rat nationallicence Valproate nationallicence Stanojlović Olivera Institute of Physiology, School of Medicine, University of Belgrade, Višegradska 26/II, 11000, Belgrade, Serbia aut Hrnčić Dragan Institute of Physiology, School of Medicine, University of Belgrade, Višegradska 26/II, 11000, Belgrade, Serbia aut Rašić Aleksandra Institute of Physiology, School of Medicine, University of Belgrade, Višegradska 26/II, 11000, Belgrade, Serbia aut Lončar-Stevanović Helena Institute of Physiology, School of Medicine, University of Belgrade, Višegradska 26/II, 11000, Belgrade, Serbia aut Djuric Dragan Institute of Physiology, School of Medicine, University of Belgrade, Višegradska 26/II, 11000, Belgrade, Serbia aut Šušić Veselinka Serbian Academy of Sciences and Arts, 11000, Belgrade, Serbia aut Cellular and Molecular Neurobiology Springer US; http://www.springer-ny.com 27/7(2007-11-01), 923-932 0272-4340 27:7<923 2007 27 10571 https://doi.org/10.1007/s10571-007-9222-5 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10571-007-9222-5 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Stanojlović Olivera Institute of Physiology, School of Medicine, University of Belgrade, Višegradska 26/II, 11000, Belgrade, Serbia aut NATIONALLICENCE 700 1- Hrnčić Dragan Institute of Physiology, School of Medicine, University of Belgrade, Višegradska 26/II, 11000, Belgrade, Serbia aut NATIONALLICENCE 700 1- Rašić Aleksandra Institute of Physiology, School of Medicine, University of Belgrade, Višegradska 26/II, 11000, Belgrade, Serbia aut NATIONALLICENCE 700 1- Lončar-Stevanović Helena Institute of Physiology, School of Medicine, University of Belgrade, Višegradska 26/II, 11000, Belgrade, Serbia aut NATIONALLICENCE 700 1- Djuric Dragan Institute of Physiology, School of Medicine, University of Belgrade, Višegradska 26/II, 11000, Belgrade, Serbia aut NATIONALLICENCE 700 1- Šušić Veselinka Serbian Academy of Sciences and Arts, 11000, Belgrade, Serbia aut NATIONALLICENCE 773 0- Cellular and Molecular Neurobiology Springer US; http://www.springer-ny.com 27/7(2007-11-01), 923-932 0272-4340 27:7<923 2007 27 10571 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer