caa a22 4500 463254325 CHVBK 20180405153350.0 cr unu---uuuuu 170326e20071101xx s 000 0 eng 10.1007/s10571-007-9188-3 doi (NATIONALLICENCE)springer-10.1007/s10571-007-9188-3 Establishment of a New Human Glioblastoma Multiforme Cell Line (WJ1) and Its Partial Characterization [Elektronische Daten] [Jing Wang, Xiujie Wang, Shu Jiang, Ping Lin, Jie Zhang, Yaying Wu, Zhujuan Xiong, Jing Ren, Hongliang Yang] (1) A new human glioblastoma multiforme (GBM) cell line, WJ1, was established from the tissue derived from a 29-year-old patient diagnosed with a grade IV GBM. (2) The WJ1 cell line has been subcultured for more than 80 passages in standard culture media without feeder layer or collagen coatings. (3) GBM cells grow invitro with distinct morphological appearance. Ultrastructural examination revealed large irregular nuclei and pseudo-inclusion bodies in nuclei. The cytoplasm contained numerous immature organelles and a few glia filaments. Growth kinetic studies demonstrated an approximate population doubling time of 60h and a colony forming efficiency of 4.04%. The karyotype of the cells was hyperdiploid, with a large subpopulation of polyploid cells. Drug sensitivities of DDP, VP-16, tanshinone IIA of this cell line were assayed. They showed a dose- and time-dependent growth inhibition effect on the cells. (4) Orthotopic transplantation of GBM cells into athymic nude mice induced the formation of solid tumor masses about 6weeks. The cells obtained from mouse tumor masses when cultivated invitro had the same morphology and ultrastructure as those of the initial cultures. (5) This cell line may provide a useful model invitro and invivo in the cellular and molecular studies as well as in testing novel therapies for human glioblastoma multiforme. Springer Science+Business Media, LLC, 2007 Glioblastoma multiforme nationallicence Brain neoplasm nationallicence Human glioma cell lines nationallicence Wang Jing Laboratory of Experimental Oncology, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, 610041, Chengdu, Sichuan, P.R. China aut Wang Xiujie Laboratory of Experimental Oncology, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, 610041, Chengdu, Sichuan, P.R. China aut Jiang Shu Neourosurgery Department, West China Hospital, Sichuan University, 610041, Chengdu, Sichuan Province, P. R. China aut Lin Ping Laboratory of Experimental Oncology, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, 610041, Chengdu, Sichuan, P.R. China aut Zhang Jie Laboratory of Experimental Oncology, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, 610041, Chengdu, Sichuan, P.R. China aut Wu Yaying Laboratory of Experimental Oncology, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, 610041, Chengdu, Sichuan, P.R. China aut Xiong Zhujuan Laboratory of Experimental Oncology, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, 610041, Chengdu, Sichuan, P.R. China aut Ren Jing Laboratory of Experimental Oncology, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, 610041, Chengdu, Sichuan, P.R. China aut Yang Hongliang Laboratory of Experimental Oncology, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, 610041, Chengdu, Sichuan, P.R. China aut Cellular and Molecular Neurobiology Springer US; http://www.springer-ny.com 27/7(2007-11-01), 831-843 0272-4340 27:7<831 2007 27 10571 https://doi.org/10.1007/s10571-007-9188-3 text/html Onlinezugriff via DOI 1 brief-communication jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10571-007-9188-3 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Wang Jing Laboratory of Experimental Oncology, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, 610041, Chengdu, Sichuan, P.R. China aut NATIONALLICENCE 700 1- Wang Xiujie Laboratory of Experimental Oncology, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, 610041, Chengdu, Sichuan, P.R. China aut NATIONALLICENCE 700 1- Jiang Shu Neourosurgery Department, West China Hospital, Sichuan University, 610041, Chengdu, Sichuan Province, P. R. China aut NATIONALLICENCE 700 1- Lin Ping Laboratory of Experimental Oncology, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, 610041, Chengdu, Sichuan, P.R. China aut NATIONALLICENCE 700 1- Zhang Jie Laboratory of Experimental Oncology, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, 610041, Chengdu, Sichuan, P.R. China aut NATIONALLICENCE 700 1- Wu Yaying Laboratory of Experimental Oncology, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, 610041, Chengdu, Sichuan, P.R. China aut NATIONALLICENCE 700 1- Xiong Zhujuan Laboratory of Experimental Oncology, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, 610041, Chengdu, Sichuan, P.R. China aut NATIONALLICENCE 700 1- Ren Jing Laboratory of Experimental Oncology, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, 610041, Chengdu, Sichuan, P.R. China aut NATIONALLICENCE 700 1- Yang Hongliang Laboratory of Experimental Oncology, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, 610041, Chengdu, Sichuan, P.R. China aut NATIONALLICENCE 773 0- Cellular and Molecular Neurobiology Springer US; http://www.springer-ny.com 27/7(2007-11-01), 831-843 0272-4340 27:7<831 2007 27 10571 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer