caa a22 4500 463254341 CHVBK 20180405153350.0 cr unu---uuuuu 170326e20071101xx s 000 0 eng 10.1007/s10571-007-9215-4 doi (NATIONALLICENCE)springer-10.1007/s10571-007-9215-4 Induction of TNF-α by LPS in Schwann Cell is Regulated by MAPK Activation Signals [Elektronische Daten] [Chun Cheng, Yongwei Qin, Xiaoyi Shao, Haibo Wang, Yongjing Gao, Mengling Cheng, Aiguo Shen] Mitogen-activated protein kinases (MAPKs) are important mediators of cytokine expression and are critically involved in the immune response. The lipopolysaccharide (LPS) of gram-negative bacteria induces the expression of cytokines and proinflammatory genes via the toll-like receptor 4 (TLR4) signaling pathway in diverse cell types. Invivo, Schwann cells (SCs) at the site of injury may also produce tumor necrosis factor-- α (TNF-α). However, the precise mechanisms of TNF-α synthesis are still not clear. The purpose of the present study was to elucidate the underlying molecular mechanisms in the cultured SCs for its ability to activate the MAPKs and TNF-α gene, in response to LPS. Using enzyme-linked immunosorbent assay (ELISA), it was confirmed that treatment with LPS stimulated the synthesis of TNF-α in a concentration- and time-dependent manner. Intracellular location of TNF-α was detected under confocal microscope. Moreover, LPS activated extracellular signal-regulated kinase (ERK1/2), P38 and stress activated protein kinase/c-Jun N-terminal kinase (SAPK/JNK) and induced their phosphorylation. LPS-elicited SCs TNF-α production was also drastically suppressed by PD98059 (ERK inhibitor), SB202190 (P38 inhibitor), or SP600125 (SAPK/JNK inhibitor). Additionally, the expression of CD14 and TLR4 was examined by RT-PCR. It was demonstrated that the expression of CD14, TLR4 was crucial for the SCs responses to LPS. In conclusion, the results provide novel mechanisms for the response of SCs to LPS stimulation, through MAPKs signaling pathways. Springer Science+Business Media, LLC, 2007 Lipopolysaccharide nationallicence Schwann cell nationallicence Tumor necrosis factor-alpha nationallicence Mitogen-activated protein kinase nationallicence Cheng Chun Key Laboratory for Neuroregeneration of JiangSu Province, Nantong University, 19 Qixiu Road, 226001, Nantong, Jiangsu, PR China aut Qin Yongwei Department of Parasitology, Medical College, Nantong University, Nantong, PR China aut Shao Xiaoyi Department of Microbiology and Immunology, Medical College, Nantong University, Nantong, PR China aut Wang Haibo Department of Microbiology and Immunology, Medical College, Nantong University, Nantong, PR China aut Gao Yongjing Institute of Nautical Medicine, Nantong University, Nantong, PR China aut Cheng Mengling Department of Microbiology and Immunology, Medical College, Nantong University, Nantong, PR China aut Shen Aiguo Key Laboratory for Neuroregeneration of JiangSu Province, Nantong University, 19 Qixiu Road, 226001, Nantong, Jiangsu, PR China aut Cellular and Molecular Neurobiology Springer US; http://www.springer-ny.com 27/7(2007-11-01), 909-921 0272-4340 27:7<909 2007 27 10571 https://doi.org/10.1007/s10571-007-9215-4 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10571-007-9215-4 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Cheng Chun Key Laboratory for Neuroregeneration of JiangSu Province, Nantong University, 19 Qixiu Road, 226001, Nantong, Jiangsu, PR China aut NATIONALLICENCE 700 1- Qin Yongwei Department of Parasitology, Medical College, Nantong University, Nantong, PR China aut NATIONALLICENCE 700 1- Shao Xiaoyi Department of Microbiology and Immunology, Medical College, Nantong University, Nantong, PR China aut NATIONALLICENCE 700 1- Wang Haibo Department of Microbiology and Immunology, Medical College, Nantong University, Nantong, PR China aut NATIONALLICENCE 700 1- Gao Yongjing Institute of Nautical Medicine, Nantong University, Nantong, PR China aut NATIONALLICENCE 700 1- Cheng Mengling Department of Microbiology and Immunology, Medical College, Nantong University, Nantong, PR China aut NATIONALLICENCE 700 1- Shen Aiguo Key Laboratory for Neuroregeneration of JiangSu Province, Nantong University, 19 Qixiu Road, 226001, Nantong, Jiangsu, PR China aut NATIONALLICENCE 773 0- Cellular and Molecular Neurobiology Springer US; http://www.springer-ny.com 27/7(2007-11-01), 909-921 0272-4340 27:7<909 2007 27 10571 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer