caa a22 4500 463254368 CHVBK 20180405153350.0 cr unu---uuuuu 170326e20071101xx s 000 0 eng 10.1007/s10571-007-9223-4 doi (NATIONALLICENCE)springer-10.1007/s10571-007-9223-4 Neuroprotection by 7-Nitroindazole Against Iron-Induced Hippocampal Neurotoxicity [Elektronische Daten] [M. Bostanci, Faruk Bağirici] (1) Iron plays an important role in maintaining normal brain function. In some neurodegenerative disorders including Parkinson's and Alzheimer's disease, iron levels rise in the brain. It is known that higher iron levels induce neuronal hyperactivity and oxidative stress. A body of evidence indicates a relationship between neuronal death and nitric oxide (NO). The aim of present study was to evaluate the effects of NO produced by neuronal nitric oxide synthase (nNOS) on iron-induced neuronal death. (2) Animals were classified into four groups: control, iron, iron+7-nitroindazole, and iron+vehicle. Rats in iron, iron+7-nitroindazole, and iron+vehicle groups received intracerebroventricular (i.c.v.) FeCl3 injection (200mM, in 2.5μl). Rats belonging to control groups received the same amount of saline into the cerebral ventricles. All animals were kept alive for 10days following the operation. Animals in iron+7-nitroindazole group received intraperitoneal 7-nitroindazole (30mg/kg/day) injections once a day during this period, while the rats belonging to vehicle group received daily intraperitoneal injection of peanut oil. After 10days, rats were perfused intracardially under deep urethane anesthesia. Removed brains were processed using the standard histological techniques. (3) The total number of neurons in hippocampus of all rats was estimated with the unbiased stereological techniques. Results of present study show that 7-nitroindazole decreased mean neuron loss from 43% to 11%. Treatment of peanut oil alone did not affect iron-induced hippocampal cell loss with respect to iron group values. (4) Findings of our study suggest that 7-nitroindazole may have neuroprotective effects against iron-induced hippocampal neurotoxicity by inhibiting nNOS. Springer Science+Business Media, LLC, 2007 Iron nationallicence Hippocampus nationallicence Cell death nationallicence 7-Nitroindazole nationallicence Stereology nationallicence Bostanci M. Department of Physiology, Faculty of Medicine, Ondokuz Mayis University, 55139, Samsun, Turkey aut Bağirici Faruk Department of Physiology, Faculty of Medicine, Ondokuz Mayis University, 55139, Samsun, Turkey aut Cellular and Molecular Neurobiology Springer US; http://www.springer-ny.com 27/7(2007-11-01), 933-941 0272-4340 27:7<933 2007 27 10571 https://doi.org/10.1007/s10571-007-9223-4 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10571-007-9223-4 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Bostanci M. Department of Physiology, Faculty of Medicine, Ondokuz Mayis University, 55139, Samsun, Turkey aut NATIONALLICENCE 700 1- Bağirici Faruk Department of Physiology, Faculty of Medicine, Ondokuz Mayis University, 55139, Samsun, Turkey aut NATIONALLICENCE 773 0- Cellular and Molecular Neurobiology Springer US; http://www.springer-ny.com 27/7(2007-11-01), 933-941 0272-4340 27:7<933 2007 27 10571 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer