caa a22 4500 463254392 CHVBK 20180405153350.0 cr unu---uuuuu 170326e20070501xx s 000 0 eng 10.1007/s10571-006-9127-8 doi (NATIONALLICENCE)springer-10.1007/s10571-006-9127-8 A β Upregulates and Colocalizes with LGI3 in Cultured Rat Astrocytes [Elektronische Daten] [Nobuyuki Kimura, Yoshiyuki Ishii, Shingo Suzaki, Takayuki Negishi, Shigeru Kyuwa, Yasuhiro Yoshikawa] : 1. The leucine-rich glioma inactivated (LGI) family of genes encodes a leucine-rich repeat (LRR) protein, proteins that are thought to be specifically involved in protein-protein and protein-matrix interactions. Since amyloid beta peptide (Aβ) has been previously shown to induce the expression of another LRR-encoding gene in neural cells, we assessed how Aβ affects LGI gene expression in rat primary cerebral cortical cultures and astrocyte cultures. Both RT-PCR and Western Blotting analyses revealed that Aβ robustly induced the expression of LGI3 in rat astrocyte cultures. 2. Western Blotting analyses also showed that both glial fibrillary acidic protein (GFAP) and apolipoprotein E (ApoE) significantly increased coincidentally with the Aβ-induced upregulation of LGI3. Immunocytochemistry showed that LGI3 colocalized with Aβ at plasma membranes and also with internalized Aβ in astrocytes. These findings suggest that activated LGI3 may be involved in the astroglial response against Aβ. Springer Science+Business Media, LLC, 2007 amyloid beta nationallicence astrocyte nationallicence leucine-rich glioma inactivated nationallicence leucine-rich repeat nationallicence Kimura Nobuyuki Laboratory of Disease Control, Tsukuba Primate Research Center, National Institute of Biomedical Innovation, Ibaraki, Japan aut Ishii Yoshiyuki Department of Biomedical Science, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, Japan aut Suzaki Shingo Department of Biomedical Science, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, Japan aut Negishi Takayuki Department of Biomedical Science, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, Japan aut Kyuwa Shigeru Department of Biomedical Science, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, Japan aut Yoshikawa Yasuhiro Department of Biomedical Science, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, Japan aut Cellular and Molecular Neurobiology Kluwer Academic Publishers-Plenum Publishers; http://www.springer-ny.com 27/3(2007-05-01), 335-350 0272-4340 27:3<335 2007 27 10571 https://doi.org/10.1007/s10571-006-9127-8 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10571-006-9127-8 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kimura Nobuyuki Laboratory of Disease Control, Tsukuba Primate Research Center, National Institute of Biomedical Innovation, Ibaraki, Japan aut NATIONALLICENCE 700 1- Ishii Yoshiyuki Department of Biomedical Science, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, Japan aut NATIONALLICENCE 700 1- Suzaki Shingo Department of Biomedical Science, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, Japan aut NATIONALLICENCE 700 1- Negishi Takayuki Department of Biomedical Science, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, Japan aut NATIONALLICENCE 700 1- Kyuwa Shigeru Department of Biomedical Science, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, Japan aut NATIONALLICENCE 700 1- Yoshikawa Yasuhiro Department of Biomedical Science, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, Japan aut NATIONALLICENCE 773 0- Cellular and Molecular Neurobiology Kluwer Academic Publishers-Plenum Publishers; http://www.springer-ny.com 27/3(2007-05-01), 335-350 0272-4340 27:3<335 2007 27 10571 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer