caa a22 4500 463254406 CHVBK 20180405153350.0 cr unu---uuuuu 170326e20070501xx s 000 0 eng 10.1007/s10571-006-9132-y doi (NATIONALLICENCE)springer-10.1007/s10571-006-9132-y Effects of Levetiracetam in Lipid Peroxidation Level, Nitrite-Nitrate Formation and Antioxidant Enzymatic Activity in Mice Brain After Pilocarpine-Induced Seizures [Elektronische Daten] [A. Oliveira, J. Almeida, R. Freitas, V. Nascimento, L. Aguiar, H. Júnior, F. Fonseca, G. Viana, F. Sousa, M. Fonteles] : Oxidative stress has been implicated in a large number of human degenerative diseases, including epilepsy. Levetiracetam (LEV) is a new antiepileptic agent with broad-spectrum effects on seizures and animal models of epilepsy. Recently, it was demonstrated that the mechanism of LEV differs from that of conventional antiepileptic drugs. Objectifying to investigate if LEV mechanism of action involves antioxidant properties, lipid peroxidation levels, nitrite-nitrate formation, catalase activity, and glutathione (GSH) content were measured in adult mice brain. The neurochemical analyses were carried out in hippocampus of animals pretreated with LEV (200mg/kg, i.p.) 60min before pilocarpine-induced seizures (400mg/kg, s.c.). The administration of alone pilocarpine, 400mg/kg, s.c. (P400) produced a significant increase of lipid peroxidation level in hippocampus. LEV pretreatment was able to counteract this increase, preserving the lipid peroxidation level in normal value. P400 administration also produced increase in the nitrite-nitrate formation and catalase activity in hippocampus, beyond a decrease in GSH levels. LEV administration before P400 prevented the P400-induced alteration in nitrite-nitrate levels and preserved normal values of catalase activity in hippocampus. Moreover, LEV administration prevented the P400-induced loss of GSH in this cerebral area. The present data suggest that the protective effects of LEV against pilocarpine-induced seizures can be mediated, at least in part, by reduction of lipid peroxidation and hippocampal oxidative stress. Springer Science+Business Media, LLC, 2006 levetiracetam nationallicence pilocarpine nationallicence lipid peroxidation nationallicence nitrite nationallicence nitrate nationallicence catalase nationallicence glutathione nationallicence hippocampus nationallicence Oliveira A. Laboratory of Neuropharmacology, Department of Physiology and Pharmacology, School of Medicine, Federal University of Ceará, Rua Cel. Nunes de Melo 1127, 60431-970, Fortaleza, CE, Brazil aut Almeida J. Laboratory of Neuropharmacology, Department of Physiology and Pharmacology, School of Medicine, Federal University of Ceará, Rua Cel. Nunes de Melo 1127, 60431-970, Fortaleza, CE, Brazil aut Freitas R. Laboratory of Neuropharmacology, Department of Physiology and Pharmacology, School of Medicine, Federal University of Ceará, Rua Cel. Nunes de Melo 1127, 60431-970, Fortaleza, CE, Brazil aut Nascimento V. Laboratory of Neuropharmacology, Department of Physiology and Pharmacology, School of Medicine, Federal University of Ceará, Rua Cel. Nunes de Melo 1127, 60431-970, Fortaleza, CE, Brazil aut Aguiar L. Laboratory of Neuropharmacology, Department of Physiology and Pharmacology, School of Medicine, Federal University of Ceará, Rua Cel. Nunes de Melo 1127, 60431-970, Fortaleza, CE, Brazil aut Júnior H. Laboratory of Neuropharmacology, Department of Physiology and Pharmacology, School of Medicine, Federal University of Ceará, Rua Cel. Nunes de Melo 1127, 60431-970, Fortaleza, CE, Brazil aut Fonseca F. Laboratory of Neuropharmacology, Department of Physiology and Pharmacology, School of Medicine, Federal University of Ceará, Rua Cel. Nunes de Melo 1127, 60431-970, Fortaleza, CE, Brazil aut Viana G. Laboratory of Neuropharmacology, Department of Physiology and Pharmacology, School of Medicine, Federal University of Ceará, Rua Cel. Nunes de Melo 1127, 60431-970, Fortaleza, CE, Brazil aut Sousa F. Laboratory of Neuropharmacology, Department of Physiology and Pharmacology, School of Medicine, Federal University of Ceará, Rua Cel. Nunes de Melo 1127, 60431-970, Fortaleza, CE, Brazil aut Fonteles M. Laboratory of Neuropharmacology, Department of Physiology and Pharmacology, School of Medicine, Federal University of Ceará, Rua Cel. Nunes de Melo 1127, 60431-970, Fortaleza, CE, Brazil aut Cellular and Molecular Neurobiology Kluwer Academic Publishers-Plenum Publishers; http://www.springer-ny.com 27/3(2007-05-01), 395-406 0272-4340 27:3<395 2007 27 10571 https://doi.org/10.1007/s10571-006-9132-y text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10571-006-9132-y text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Oliveira A. Laboratory of Neuropharmacology, Department of Physiology and Pharmacology, School of Medicine, Federal University of Ceará, Rua Cel. Nunes de Melo 1127, 60431-970, Fortaleza, CE, Brazil aut NATIONALLICENCE 700 1- Almeida J. Laboratory of Neuropharmacology, Department of Physiology and Pharmacology, School of Medicine, Federal University of Ceará, Rua Cel. Nunes de Melo 1127, 60431-970, Fortaleza, CE, Brazil aut NATIONALLICENCE 700 1- Freitas R. Laboratory of Neuropharmacology, Department of Physiology and Pharmacology, School of Medicine, Federal University of Ceará, Rua Cel. Nunes de Melo 1127, 60431-970, Fortaleza, CE, Brazil aut NATIONALLICENCE 700 1- Nascimento V. Laboratory of Neuropharmacology, Department of Physiology and Pharmacology, School of Medicine, Federal University of Ceará, Rua Cel. Nunes de Melo 1127, 60431-970, Fortaleza, CE, Brazil aut NATIONALLICENCE 700 1- Aguiar L. Laboratory of Neuropharmacology, Department of Physiology and Pharmacology, School of Medicine, Federal University of Ceará, Rua Cel. Nunes de Melo 1127, 60431-970, Fortaleza, CE, Brazil aut NATIONALLICENCE 700 1- Júnior H. Laboratory of Neuropharmacology, Department of Physiology and Pharmacology, School of Medicine, Federal University of Ceará, Rua Cel. Nunes de Melo 1127, 60431-970, Fortaleza, CE, Brazil aut NATIONALLICENCE 700 1- Fonseca F. Laboratory of Neuropharmacology, Department of Physiology and Pharmacology, School of Medicine, Federal University of Ceará, Rua Cel. Nunes de Melo 1127, 60431-970, Fortaleza, CE, Brazil aut NATIONALLICENCE 700 1- Viana G. Laboratory of Neuropharmacology, Department of Physiology and Pharmacology, School of Medicine, Federal University of Ceará, Rua Cel. Nunes de Melo 1127, 60431-970, Fortaleza, CE, Brazil aut NATIONALLICENCE 700 1- Sousa F. Laboratory of Neuropharmacology, Department of Physiology and Pharmacology, School of Medicine, Federal University of Ceará, Rua Cel. Nunes de Melo 1127, 60431-970, Fortaleza, CE, Brazil aut NATIONALLICENCE 700 1- Fonteles M. Laboratory of Neuropharmacology, Department of Physiology and Pharmacology, School of Medicine, Federal University of Ceará, Rua Cel. Nunes de Melo 1127, 60431-970, Fortaleza, CE, Brazil aut NATIONALLICENCE 773 0- Cellular and Molecular Neurobiology Kluwer Academic Publishers-Plenum Publishers; http://www.springer-ny.com 27/3(2007-05-01), 395-406 0272-4340 27:3<395 2007 27 10571 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer