caa a22 4500 463254414 CHVBK 20180405153350.0 cr unu---uuuuu 170326e20070501xx s 000 0 eng 10.1007/s10571-006-9125-x doi (NATIONALLICENCE)springer-10.1007/s10571-006-9125-x Inhibition of Transforming Growth Factor- β Production in Brain Pericytes Contributes to Cyclosporin A-Induced Dysfunction of the Blood-Brain Barrier [Elektronische Daten] [Fuyuko Takata, Shinya Dohgu, Atsushi Yamauchi, Noriko Sumi, Shinsuke Nakagawa, Mikihiko Naito, Takashi Tsuruo, Hideki Shuto, Yasufumi Kataoka] : 1. The present study was designed to clarify whether brain pericytes and pericyte-derived transforming growth factor-β1 (TGF-β1) participate in cyclosporin A (CsA)-induced dysfunction of the blood-brain barrier (BBB). 2. The presence of brain pericytes markedly aggravated CsA-increased permeability of MBEC4 cells to sodium fluorescein and accumulation of rhodamine 123 in MBEC4 cells. 3. Exposure to CsA significantly decreased the levels of TGF-β1 mRNA in brain pericytes in pericyte co-cultures. Treatment with TGF-β1 dose-dependently inhibited CsA-induced hyperpermeability and P-glycoprotein dysfunction of MBEC4 cells in pericyte co-cultures. 4. These findings suggest that an inhibition of brain pericyte-derived TGF-β1 contributes to the occurrence of CsA-induced dysfunction of the BBB. Springer Science+Business Media, LLC, 2006 Cyclosporin A nationallicence brain pericytes nationallicence transforming growth factor-β nationallicence blood-brain barrier nationallicence permeability nationallicence P-glycoprotein nationallicence mouse brain endothelial cells nationallicence Takata Fuyuko Department of Pharmaceutical Care and Health Sciences, Faculty of Pharmaceutical Sciences, Fukuoka University, Jonan-ku, 814-0180, Fukuoka, Japan aut Dohgu Shinya Department of Pharmaceutical Care and Health Sciences, Faculty of Pharmaceutical Sciences, Fukuoka University, Jonan-ku, 814-0180, Fukuoka, Japan aut Yamauchi Atsushi Department of Pharmaceutical Care and Health Sciences, Faculty of Pharmaceutical Sciences, Fukuoka University, Jonan-ku, 814-0180, Fukuoka, Japan aut Sumi Noriko Department of Pharmaceutical Care and Health Sciences, Faculty of Pharmaceutical Sciences, Fukuoka University, Jonan-ku, 814-0180, Fukuoka, Japan aut Nakagawa Shinsuke PharmaCo-Cell Company Ltd., Nagayo-machi, 851-2127, Nagasaki, Japan aut Naito Mikihiko Institute of Molecular and Cellular Biosciences, University of Tokyo, Bunkyo-ku, 113-0032, Tokyo, Japan aut Tsuruo Takashi Institute of Molecular and Cellular Biosciences, University of Tokyo, Bunkyo-ku, 113-0032, Tokyo, Japan aut Shuto Hideki Department of Pharmaceutical Care and Health Sciences, Faculty of Pharmaceutical Sciences, Fukuoka University, Jonan-ku, 814-0180, Fukuoka, Japan aut Kataoka Yasufumi Department of Pharmaceutical Care and Health Sciences, Faculty of Pharmaceutical Sciences, Fukuoka University, Jonan-ku, 814-0180, Fukuoka, Japan aut Cellular and Molecular Neurobiology Kluwer Academic Publishers-Plenum Publishers; http://www.springer-ny.com 27/3(2007-05-01), 317-328 0272-4340 27:3<317 2007 27 10571 https://doi.org/10.1007/s10571-006-9125-x text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10571-006-9125-x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Takata Fuyuko Department of Pharmaceutical Care and Health Sciences, Faculty of Pharmaceutical Sciences, Fukuoka University, Jonan-ku, 814-0180, Fukuoka, Japan aut NATIONALLICENCE 700 1- Dohgu Shinya Department of Pharmaceutical Care and Health Sciences, Faculty of Pharmaceutical Sciences, Fukuoka University, Jonan-ku, 814-0180, Fukuoka, Japan aut NATIONALLICENCE 700 1- Yamauchi Atsushi Department of Pharmaceutical Care and Health Sciences, Faculty of Pharmaceutical Sciences, Fukuoka University, Jonan-ku, 814-0180, Fukuoka, Japan aut NATIONALLICENCE 700 1- Sumi Noriko Department of Pharmaceutical Care and Health Sciences, Faculty of Pharmaceutical Sciences, Fukuoka University, Jonan-ku, 814-0180, Fukuoka, Japan aut NATIONALLICENCE 700 1- Nakagawa Shinsuke PharmaCo-Cell Company Ltd., Nagayo-machi, 851-2127, Nagasaki, Japan aut NATIONALLICENCE 700 1- Naito Mikihiko Institute of Molecular and Cellular Biosciences, University of Tokyo, Bunkyo-ku, 113-0032, Tokyo, Japan aut NATIONALLICENCE 700 1- Tsuruo Takashi Institute of Molecular and Cellular Biosciences, University of Tokyo, Bunkyo-ku, 113-0032, Tokyo, Japan aut NATIONALLICENCE 700 1- Shuto Hideki Department of Pharmaceutical Care and Health Sciences, Faculty of Pharmaceutical Sciences, Fukuoka University, Jonan-ku, 814-0180, Fukuoka, Japan aut NATIONALLICENCE 700 1- Kataoka Yasufumi Department of Pharmaceutical Care and Health Sciences, Faculty of Pharmaceutical Sciences, Fukuoka University, Jonan-ku, 814-0180, Fukuoka, Japan aut NATIONALLICENCE 773 0- Cellular and Molecular Neurobiology Kluwer Academic Publishers-Plenum Publishers; http://www.springer-ny.com 27/3(2007-05-01), 317-328 0272-4340 27:3<317 2007 27 10571 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer