caa a22 4500 463254430 CHVBK 20180405153350.0 cr unu---uuuuu 170326e20070501xx s 000 0 eng 10.1007/s10571-006-9108-y doi (NATIONALLICENCE)springer-10.1007/s10571-006-9108-y Antibodies to a Nonconjugated Prion Protein Peptide 95-123 Interfere with PrP Sc Propagation in Prion-Infected Cells [Elektronische Daten] [Maria Oboznaya, Sabine Gilch, Maia Titova, Dmitry Koroev, Tatyana Volkova, Olga Volpina, Hermann Schätzl] Summary: 1. Vaccination-induced anti-prion protein antibodies are presently regarded as a promising approach toward treatment of prion diseases. Here, we investigated the ability of five peptides corresponding to three different regions of the bovine prion protein (PrP) to elicit antibodies interfering with PrPSc propagation in prion-infected cells. 2. Rabbits were immunized with free nonconjugated peptides. Obtained immune sera were tested in enzyme-linked immunosorbent assay (ELISA) and immunoblot for their binding to recombinant PrP and cell-derived pathogenic isoform (PrPSc) and normal prion protein (PrPc), respectively. Sera positive in all tests were chosen for PrPSc inhibition studies in cell culture. 3. All peptides induced anti-peptide antibodies, most of them reacting with recombinant PrP. Moreover, addition of the serum specific to peptide 95-123 led to a transient reduction of PrPSc levels in persistently prion-infected cells. 4. Thus, anti-PrP antibodies interfering with PrPSc propagation were induced with a prion protein peptide nonconjugated to a protein carrier. These results point to the potential application of the nonconjugated peptide 95-123 for the treatment of prion diseases. Springer Science+Business Media, Inc., 2006 prion protein nationallicence prion peptide nationallicence nonconjugated peptide nationallicence anti-peptide antibodies nationallicence Oboznaya Maria Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, ul. Miklukho-Maklaya 16/10, 117997, GSP Moscow, Russia aut Gilch Sabine Technical University of Munich, Institute of Virology/Prion Research Group, Trogerstrasse 30, 81675, Munich, Germany aut Titova Maia Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, ul. Miklukho-Maklaya 16/10, 117997, GSP Moscow, Russia aut Koroev Dmitry Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, ul. Miklukho-Maklaya 16/10, 117997, GSP Moscow, Russia aut Volkova Tatyana Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, ul. Miklukho-Maklaya 16/10, 117997, GSP Moscow, Russia aut Volpina Olga Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, ul. Miklukho-Maklaya 16/10, 117997, GSP Moscow, Russia aut Schätzl Hermann Technical University of Munich, Institute of Virology/Prion Research Group, Trogerstrasse 30, 81675, Munich, Germany aut Cellular and Molecular Neurobiology Kluwer Academic Publishers-Plenum Publishers; http://www.springer-ny.com 27/3(2007-05-01), 271-284 0272-4340 27:3<271 2007 27 10571 https://doi.org/10.1007/s10571-006-9108-y text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10571-006-9108-y text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Oboznaya Maria Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, ul. Miklukho-Maklaya 16/10, 117997, GSP Moscow, Russia aut NATIONALLICENCE 700 1- Gilch Sabine Technical University of Munich, Institute of Virology/Prion Research Group, Trogerstrasse 30, 81675, Munich, Germany aut NATIONALLICENCE 700 1- Titova Maia Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, ul. Miklukho-Maklaya 16/10, 117997, GSP Moscow, Russia aut NATIONALLICENCE 700 1- Koroev Dmitry Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, ul. Miklukho-Maklaya 16/10, 117997, GSP Moscow, Russia aut NATIONALLICENCE 700 1- Volkova Tatyana Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, ul. Miklukho-Maklaya 16/10, 117997, GSP Moscow, Russia aut NATIONALLICENCE 700 1- Volpina Olga Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, ul. Miklukho-Maklaya 16/10, 117997, GSP Moscow, Russia aut NATIONALLICENCE 700 1- Schätzl Hermann Technical University of Munich, Institute of Virology/Prion Research Group, Trogerstrasse 30, 81675, Munich, Germany aut NATIONALLICENCE 773 0- Cellular and Molecular Neurobiology Kluwer Academic Publishers-Plenum Publishers; http://www.springer-ny.com 27/3(2007-05-01), 271-284 0272-4340 27:3<271 2007 27 10571 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer