caa a22 4500 463254473 CHVBK 20180405153350.0 cr unu---uuuuu 170326e20070501xx s 000 0 eng 10.1007/s10571-006-9129-6 doi (NATIONALLICENCE)springer-10.1007/s10571-006-9129-6 Bakalova Rumiana On-Site Sensing and Diagnosis Research Laboratory, AIST-Kyushy, 807-1 Shuku-machi, 841-0052, Tosu, Saga, Japan aut Ultra-Fast Biosensors and Multi-Photon Microscopy in the Future of Brain Studies [Elektronische Daten] [Rumiana Bakalova] The direct, highly selective and sensitive real-time imaging of neuro- and biochemical mediators is the only way to clarify precisely the chemistry of the brain and to discover the key molecular targets involved in regulation of brain homeostasis. To realize that, we need: high-speed deep-tissue imaging techniques with high spatial and temporal resolution; and ultra-fast and highly selective molecular sensors, giving a possibility to monitor target molecules directly in their physiological environment; in addition, these molecular sensors have to be comparatively small and permeable for blood-brain barrier, to be applicable in brain studies. The present view accents on the perspectives for development of direct approach for investigation of function/flow coupling phenomenon in the brain, based on the current progress in development of ultra-fast molecular sensors for direct visualization of biochemical mediators (e.g., nitric oxide, Ca ions), and high-speed two-photon/multi-photon deep-tissue imaging. Springer Science+Business Media, LLC, 2006 neuroscience nationallicence function/flow coupling nationallicence multi-photon microscopy nationallicence biosensors nationallicence Cellular and Molecular Neurobiology Kluwer Academic Publishers-Plenum Publishers; http://www.springer-ny.com 27/3(2007-05-01), 359-365 0272-4340 27:3<359 2007 27 10571 https://doi.org/10.1007/s10571-006-9129-6 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10571-006-9129-6 text/html Onlinezugriff via DOI NATIONALLICENCE 100 1- Bakalova Rumiana On-Site Sensing and Diagnosis Research Laboratory, AIST-Kyushy, 807-1 Shuku-machi, 841-0052, Tosu, Saga, Japan aut NATIONALLICENCE 773 0- Cellular and Molecular Neurobiology Kluwer Academic Publishers-Plenum Publishers; http://www.springer-ny.com 27/3(2007-05-01), 359-365 0272-4340 27:3<359 2007 27 10571 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer