caa a22 4500 463254481 CHVBK 20180405153350.0 cr unu---uuuuu 170326e20070501xx s 000 0 eng 10.1007/s10571-007-9139-z doi (NATIONALLICENCE)springer-10.1007/s10571-007-9139-z An Inhibitory Role of Nitric Oxide in the Dynamic Regulation of the Blood-Brain Barrier Function [Elektronische Daten] [Atsushi Yamauchi, Shinya Dohgu, Tsuyoshi Nishioku, Hideki Shuto, Mikihiko Naito, Takashi Tsuruo, Yasufumi Sawada, Yasufumi Kataoka] 1. The present study aimed at elucidating the effect of nitric oxide (NO) on blood-brain barrier (BBB) function with mouse brain capillary endothelial (MBEC4) cells. 2. Histamine (20-100μM) evoked NO production (1.6-7μM) in MBEC4 cells in a dose-dependent manner. 3. The permeability coefficient of sodium fluorescein for MBEC4 cells and the cellular accumulation of rhodamine 123 in MBEC4 cells were increased dose-dependently by the addition of NO solutions (14 and 28μM) every 10min during a 30-min period. 4. The present study demonstrated that NO increased the permeability and inhibited the P-glycoprotein efflux pump of brain capillary endothelial cells, suggesting that NO plays an inhibitory role in the dynamic regulation of the BBB function. Springer Science+Business Media, LLC, 2007 nitric oxide nationallicence blood-brain barrier (BBB) nationallicence permeability nationallicence P-glycoprotein nationallicence mouse brain endothelial cells nationallicence Yamauchi Atsushi Department of Pharmaceutical Care and Health Sciences, Faculty of Pharmaceutical Sciences, Fukuoka University, 8-19-1 Nanakuma, 814-0180, Jonan-ku, Fukuoka, Japan aut Dohgu Shinya Department of Pharmaceutical Care and Health Sciences, Faculty of Pharmaceutical Sciences, Fukuoka University, 8-19-1 Nanakuma, 814-0180, Jonan-ku, Fukuoka, Japan aut Nishioku Tsuyoshi Department of Pharmaceutical Care and Health Sciences, Faculty of Pharmaceutical Sciences, Fukuoka University, 8-19-1 Nanakuma, 814-0180, Jonan-ku, Fukuoka, Japan aut Shuto Hideki Department of Pharmaceutical Care and Health Sciences, Faculty of Pharmaceutical Sciences, Fukuoka University, 8-19-1 Nanakuma, 814-0180, Jonan-ku, Fukuoka, Japan aut Naito Mikihiko Institute of Molecular and Cellular Biosciences, University of Tokyo, 113-0032, Bunkyo-ku, Tokyo, Japan aut Tsuruo Takashi Institute of Molecular and Cellular Biosciences, University of Tokyo, 113-0032, Bunkyo-ku, Tokyo, Japan aut Sawada Yasufumi Laboratory of Drug Imformatics, Graduate School of Pharmaceutical Sciences, The University of Tokyo, 3-14-15 Hongo, 113-0033, Bunkyo-ku, Tokyo, Japan aut Kataoka Yasufumi Department of Pharmaceutical Care and Health Sciences, Faculty of Pharmaceutical Sciences, Fukuoka University, 8-19-1 Nanakuma, 814-0180, Jonan-ku, Fukuoka, Japan aut Cellular and Molecular Neurobiology Kluwer Academic Publishers-Plenum Publishers; http://www.springer-ny.com 27/3(2007-05-01), 263-270 0272-4340 27:3<263 2007 27 10571 https://doi.org/10.1007/s10571-007-9139-z text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10571-007-9139-z text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Yamauchi Atsushi Department of Pharmaceutical Care and Health Sciences, Faculty of Pharmaceutical Sciences, Fukuoka University, 8-19-1 Nanakuma, 814-0180, Jonan-ku, Fukuoka, Japan aut NATIONALLICENCE 700 1- Dohgu Shinya Department of Pharmaceutical Care and Health Sciences, Faculty of Pharmaceutical Sciences, Fukuoka University, 8-19-1 Nanakuma, 814-0180, Jonan-ku, Fukuoka, Japan aut NATIONALLICENCE 700 1- Nishioku Tsuyoshi Department of Pharmaceutical Care and Health Sciences, Faculty of Pharmaceutical Sciences, Fukuoka University, 8-19-1 Nanakuma, 814-0180, Jonan-ku, Fukuoka, Japan aut NATIONALLICENCE 700 1- Shuto Hideki Department of Pharmaceutical Care and Health Sciences, Faculty of Pharmaceutical Sciences, Fukuoka University, 8-19-1 Nanakuma, 814-0180, Jonan-ku, Fukuoka, Japan aut NATIONALLICENCE 700 1- Naito Mikihiko Institute of Molecular and Cellular Biosciences, University of Tokyo, 113-0032, Bunkyo-ku, Tokyo, Japan aut NATIONALLICENCE 700 1- Tsuruo Takashi Institute of Molecular and Cellular Biosciences, University of Tokyo, 113-0032, Bunkyo-ku, Tokyo, Japan aut NATIONALLICENCE 700 1- Sawada Yasufumi Laboratory of Drug Imformatics, Graduate School of Pharmaceutical Sciences, The University of Tokyo, 3-14-15 Hongo, 113-0033, Bunkyo-ku, Tokyo, Japan aut NATIONALLICENCE 700 1- Kataoka Yasufumi Department of Pharmaceutical Care and Health Sciences, Faculty of Pharmaceutical Sciences, Fukuoka University, 8-19-1 Nanakuma, 814-0180, Jonan-ku, Fukuoka, Japan aut NATIONALLICENCE 773 0- Cellular and Molecular Neurobiology Kluwer Academic Publishers-Plenum Publishers; http://www.springer-ny.com 27/3(2007-05-01), 263-270 0272-4340 27:3<263 2007 27 10571 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer