caa a22 4500 463254546 CHVBK 20180405153350.0 cr unu---uuuuu 170326e20070601xx s 000 0 eng 10.1007/s10571-007-9140-6 doi (NATIONALLICENCE)springer-10.1007/s10571-007-9140-6 The Distribution of Primary Nitric Oxide Synthase- and Parvalbumin- Immunoreactive Afferents in the Dorsal Funiculus of the Lumbosacral Spinal Cord in a Dog [Elektronische Daten] [Jozef Maršala, Nadežda Lukáčová, Dalibor Kolesár, Igor Šulla, Ján Gálik, Martin Maršala] 1. The aim of the present study was to examine the distribution of unmyelinated, small-diameter myelinated neuronal nitric oxide synthase immunoreactive (nNOS-IR) axons and large-diameter myelinated neuronal nitric oxide synthase and parvalbumin-immunoreactive (PV-IR) axons in the dorsal funiculus (DF) of sacral (S1-S3) and lumbar (L1-L7) segments of the dog. 2. nNOS and PV immunohistochemical methods were used to demonstrate the presence of nNOS-IR and PV-IR in the large-diameter myelinated, presumed to be proprioceptive, axons in the DF along the lumbosacral segments. 3. Fiber size and density of nNOS-IR and PV-IR axons were used to compartmentalize the DF into five compartments (CI-CV). The first compartment (CI) localized in the lateralmost part of the DF, containing both unmyelinated and small-diameter myelinated nNOS-IR axons, is homologous with the dorsolateral fasciculus, or Lissauer tract. The second compartment (CII) having similar fiber organization as CI is situated more medially in sacral segments. Rostrally, in lower lumbar segments, CII moves more medially, and at upper lumbar level, CII reaches the dorsomedial angle of the DF and fuses with axons of CIV. CIII is the largest in the DF and the only one containing large-diameter myelinated nNOS-IR and PV-IR axons. The largest nNOS-IR and PV-IR axons of CIII (8.0-9.2μm in diameter), presumed to be stem Ia proprioceptive afferents, are located in the deep portion of the DF close to the dorsal and dorsomedial border of the dorsal horn. The CIV compartment varies in shape, appearing first as a small triangular area in S3 and S2 segments, homologous with the Philippe-Gombault triangle. Beginning at S1 level, CIV acquires a more elongated shape and is seen throughout the lumbar segments as a narrow band of fibers extending just below the dorsal median septum in approximately upper two-thirds of the DF. The CV is located in the basal part of the DF. In general, CV is poor in nNOS-IR fibers; among them solitary PV-IR fibers are seen. 4. The analysis of the control material and the degeneration of the large- and medium-caliber nNOS-IR fibers after unilateral L7 and S1 dorsal rhizotomy confirmed that large-caliber nNOS-IR and and PV-IR axons, presumed to be proprioceptive Ia axons, and their ascending and descending collaterals are present in large number in the DF of the lumbosacral intumescence. However, in the DF of the upper lumbar segments, the decrease in the number of nNOS-IR and PV-IR fibers is quite evident. Springer Science+Business Media, LLC, 2007 primary afferents nationallicence dorsal funiculus nationallicence neuronal nitric oxide synthase nationallicence parvalbumin nationallicence immunoreactivity nationallicence CI-CV : fibre compartments in DF nationallicence DF : dorsal funiculus nationallicence DRG : dorsal root ganglion nationallicence DREZ-one : dorsal root entry zone nationallicence eNOS : endothelial nitric oxide synthase nationallicence LB : lateral bundle of the dorsal root nationallicence LS : lumbosacral segments nationallicence MB : medial bundle of the dorsal root nationallicence mNOS : macrophage nitric oxide synthase nationallicence NADPHd : nicotinamide adenine dinucleotide phosphate diaphorase nationallicence nNOS-IR : neuronal nitric oxide synthase immunoreactivity nationallicence PV-IR : parvalbumin immunoreactivity nationallicence TPG : the triangle of Philippe-Gombault nationallicence Maršala Jozef Institute of Neurobiology, Slovak Academy of Sciences, Šoltésovej 4, 040 01, Košice, Slovak Republic aut Lukáčová Nadežda Institute of Neurobiology, Slovak Academy of Sciences, Šoltésovej 4, 040 01, Košice, Slovak Republic aut Kolesár Dalibor Institute of Neurobiology, Slovak Academy of Sciences, Šoltésovej 4, 040 01, Košice, Slovak Republic aut Šulla Igor Department of Neurosurgery, Medical Faculty of P. J. Šafárik University, 040 01, Košice, Slovak Republic aut Gálik Ján Institute of Neurobiology, Slovak Academy of Sciences, Šoltésovej 4, 040 01, Košice, Slovak Republic aut Maršala Martin Anesthesiology Research Laboratory, University of California, 92093-0818, San Diego, CA, USA aut Cellular and Molecular Neurobiology Kluwer Academic Publishers-Plenum Publishers; http://www.springer-ny.com 27/4(2007-06-01), 475-504 0272-4340 27:4<475 2007 27 10571 https://doi.org/10.1007/s10571-007-9140-6 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10571-007-9140-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Maršala Jozef Institute of Neurobiology, Slovak Academy of Sciences, Šoltésovej 4, 040 01, Košice, Slovak Republic aut NATIONALLICENCE 700 1- Lukáčová Nadežda Institute of Neurobiology, Slovak Academy of Sciences, Šoltésovej 4, 040 01, Košice, Slovak Republic aut NATIONALLICENCE 700 1- Kolesár Dalibor Institute of Neurobiology, Slovak Academy of Sciences, Šoltésovej 4, 040 01, Košice, Slovak Republic aut NATIONALLICENCE 700 1- Šulla Igor Department of Neurosurgery, Medical Faculty of P. J. Šafárik University, 040 01, Košice, Slovak Republic aut NATIONALLICENCE 700 1- Gálik Ján Institute of Neurobiology, Slovak Academy of Sciences, Šoltésovej 4, 040 01, Košice, Slovak Republic aut NATIONALLICENCE 700 1- Maršala Martin Anesthesiology Research Laboratory, University of California, 92093-0818, San Diego, CA, USA aut NATIONALLICENCE 773 0- Cellular and Molecular Neurobiology Kluwer Academic Publishers-Plenum Publishers; http://www.springer-ny.com 27/4(2007-06-01), 475-504 0272-4340 27:4<475 2007 27 10571 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer