caa a22 4500 463254562 CHVBK 20180405153351.0 cr unu---uuuuu 170326e20070601xx s 000 0 eng 10.1007/s10571-007-9138-0 doi (NATIONALLICENCE)springer-10.1007/s10571-007-9138-0 Membrane Organization of the Serotonin 1A Receptor Monitored by a Detergent-Free Approach [Elektronische Daten] [Shanti Kalipatnapu, Amitabha Chattopadhyay] : 1. Insolubility of membrane constituents in nonionic detergents such as Triton X-100 has been a widely used biochemical criterion to indicate their localization in membrane domains. However, concerns on the possibility of membrane perturbation in the presence of detergents have led to the development of detergent-free approaches. 2. We have explored the organization of the serotonin1A receptor, an important G-protein coupled receptor, from bovine hippocampus and CHO cells using a detergent-free approach in order to address the points of agreement with our previous results using Triton X-100. 3. A significant fraction of the serotonin1A receptor has been found to be localized in a heavy density fraction obtained using a detergent-free approach to isolate membrane domains. In addition, we have characterized the membrane fractions isolated in terms of their lipid composition and membrane physical properties. 4. The results obtained on the membrane localization of the serotonin1A receptor from the present experiments using a detergent-free approach correlate well with our earlier findings obtained using a detergent-based method (Kalipatnapu, S., and Chattopadhyay, A., FEBS Lett. 576:455-460, 2004). These results provide important information on the membrane organization of the hippocampal serotonin1A receptor and are relevant in view of the concerns on the use of detergent in determination of membrane organization of constituent proteins and lipids. Springer Science+Business Media, LLC, 2007 serotonin1A receptor nationallicence membrane organization nationallicence detergent-free method nationallicence bovine hippocampus nationallicence Kalipatnapu Shanti Centre for Cellular and Molecular Biology, Uppal Road, Hyderabad 500 007, India aut Chattopadhyay Amitabha Centre for Cellular and Molecular Biology, Uppal Road, Hyderabad 500 007, India aut Cellular and Molecular Neurobiology Kluwer Academic Publishers-Plenum Publishers; http://www.springer-ny.com 27/4(2007-06-01), 463-474 0272-4340 27:4<463 2007 27 10571 https://doi.org/10.1007/s10571-007-9138-0 text/html Onlinezugriff via DOI 1 brief-communication jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10571-007-9138-0 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kalipatnapu Shanti Centre for Cellular and Molecular Biology, Uppal Road, Hyderabad 500 007, India aut NATIONALLICENCE 700 1- Chattopadhyay Amitabha Centre for Cellular and Molecular Biology, Uppal Road, Hyderabad 500 007, India aut NATIONALLICENCE 773 0- Cellular and Molecular Neurobiology Kluwer Academic Publishers-Plenum Publishers; http://www.springer-ny.com 27/4(2007-06-01), 463-474 0272-4340 27:4<463 2007 27 10571 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer