caa a22 4500 463254635 CHVBK 20180405153351.0 cr unu---uuuuu 170326e20071201xx s 000 0 eng 10.1007/s10571-007-9221-6 doi (NATIONALLICENCE)springer-10.1007/s10571-007-9221-6 FK506 Protects Neurons Following Peripheral Nerve Injury Via Immunosuppression [Elektronische Daten] [Kapil Saxena, Nisha Patro, Ishan Patro] In this study, we have evaluated neuroprotective effect of an immunosuppressant immunophilin ligand, FK506, in the sciatic nerve injury model in rats. FK506 was injected to the sciatic nerve transected 3-month-old female Wistar rats (2mg/kg/day starting 1day prior to sciatic nerve injury up to 7day post operation). Equal number of sciatic nerve transected animals served as injured untreated controls. The contralateral side served as respective control. L4-L5 region of the spinal cord was removed on day 1, 3, 7, 14, 21, and 28, post operation and then processed for cryo-sectioning and paraffin sectioning. The cryocut sections were used for immunohistochemistry for localizing all microglia (using anti-Iba-1) and MHC-II expressing microglia (with OX-6). The physical dissector method was applied on Nissl stained paraffin sections for absolute motor neuron counting in the L4-L5 region of spinal cord. FK506 treated animals presented 88.7% neuronal survival while the injured alone had 79.12%, which is significantly less than the treated animals. FK506 caused early proliferation of microglia at 1 and 3days post operation. FK506 also significantly restricted transformation of these cells in to phagocytes. Colocalization of activated microglia by anti-Iba-1 and OX-6 antibodies, confirms that the MHC-II expressing cells in injured spinal cord are none other than microglial cells and MHC-II expressing cells are significantly less in treated as compared to untreated injured animals. We propose that immunosuppression is one of the main mechanisms by which FK506 protects the central neurons following peripheral injury. Springer Science+Business Media, LLC, 2007 FK506 nationallicence Microglia nationallicence Sciatic nerve injury nationallicence Immunosuppression nationallicence Neuroprotection nationallicence Saxena Kapil School of Studies in Neuroscience, Jiwaji University, 474011, Gwalior, Madhya Pradesh, India aut Patro Nisha School of Studies in Neuroscience, Jiwaji University, 474011, Gwalior, Madhya Pradesh, India aut Patro Ishan School of Studies in Neuroscience, Jiwaji University, 474011, Gwalior, Madhya Pradesh, India aut Cellular and Molecular Neurobiology Springer US; http://www.springer-ny.com 27/8(2007-12-01), 1049-1057 0272-4340 27:8<1049 2007 27 10571 https://doi.org/10.1007/s10571-007-9221-6 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10571-007-9221-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Saxena Kapil School of Studies in Neuroscience, Jiwaji University, 474011, Gwalior, Madhya Pradesh, India aut NATIONALLICENCE 700 1- Patro Nisha School of Studies in Neuroscience, Jiwaji University, 474011, Gwalior, Madhya Pradesh, India aut NATIONALLICENCE 700 1- Patro Ishan School of Studies in Neuroscience, Jiwaji University, 474011, Gwalior, Madhya Pradesh, India aut NATIONALLICENCE 773 0- Cellular and Molecular Neurobiology Springer US; http://www.springer-ny.com 27/8(2007-12-01), 1049-1057 0272-4340 27:8<1049 2007 27 10571 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer