caa a22 4500 463254759 CHVBK 20180405153351.0 cr unu---uuuuu 170326e20070701xx s 000 0 eng 10.1007/s10571-007-9156-y doi (NATIONALLICENCE)springer-10.1007/s10571-007-9156-y Multicore Vesicles: Hyperosmolarity and l -DOPA Induce Homotypic Fusion of Dense Core Vesicles [Elektronische Daten] [Leslie Sombers, Marc Maxson, Andrew Ewing] Aim: A fraction of vesicles in cells treated with hypertonic solution exhibit multiple dense cores and this is enhanced by treatment with l-3,4-dihydroxyphenylalanine (l-DOPA). These cells were examined to determine if the multicore vesicles are the product of endocytosis or homotypic fusion. Methods: Electron microscopy was used to determine the number of multicore vesicles and amperometry was used to examine if the multicore vesicles are a competent fraction of the readily releasable pool. Results: In this study, we observed that a substantial portion (15.3%) of large dense core vesicles in PC12 cells contained multiple cores in hypertonic saline loaded with l-DOPA, and amperometric measurements show a bimodal distribution of quantal sizes in treated cells. Conclusions: The results suggest that the multicored vesicles are formed from homotypic fusion of LCDVs prior to exocytosis. Springer Science+Business Media, LLC, 2007 Exocytosis nationallicence Secretory granules nationallicence Electron microscopy nationallicence PC12 nationallicence Cationized ferritin nationallicence Quantal size nationallicence Amperometry nationallicence Catecholamines nationallicence PC12 : Rat Pheochromocytoma nationallicence TEM : Transmission Electron Microscopy nationallicence l -DOPA : l-3,4-dihydroxyphenylalanine nationallicence VMAT : Vesicular Monoamine Transporter nationallicence AL : Attoliters (10−18l) nationallicence Sombers Leslie Department of Chemistry, The Pennsylvania State University, 104 Chemistry Research Building, 16802-6300, University Park, PA, USA aut Maxson Marc Huck Institutes of the Life Sciences, The Pennsylvania State University, 16802, University Park, PA, USA aut Ewing Andrew Department of Chemistry, The Pennsylvania State University, 104 Chemistry Research Building, 16802-6300, University Park, PA, USA aut Cellular and Molecular Neurobiology Kluwer Academic Publishers-Plenum Publishers; http://www.springer-ny.com 27/5(2007-07-01), 681-685 0272-4340 27:5<681 2007 27 10571 https://doi.org/10.1007/s10571-007-9156-y text/html Onlinezugriff via DOI 1 brief-communication jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10571-007-9156-y text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Sombers Leslie Department of Chemistry, The Pennsylvania State University, 104 Chemistry Research Building, 16802-6300, University Park, PA, USA aut NATIONALLICENCE 700 1- Maxson Marc Huck Institutes of the Life Sciences, The Pennsylvania State University, 16802, University Park, PA, USA aut NATIONALLICENCE 700 1- Ewing Andrew Department of Chemistry, The Pennsylvania State University, 104 Chemistry Research Building, 16802-6300, University Park, PA, USA aut NATIONALLICENCE 773 0- Cellular and Molecular Neurobiology Kluwer Academic Publishers-Plenum Publishers; http://www.springer-ny.com 27/5(2007-07-01), 681-685 0272-4340 27:5<681 2007 27 10571 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer