caa a22 4500 463254767 CHVBK 20180405153351.0 cr unu---uuuuu 170326e20070701xx s 000 0 eng 10.1007/s10571-007-9153-1 doi (NATIONALLICENCE)springer-10.1007/s10571-007-9153-1 Editing of AMPA and Serotonin 2C Receptors in Individual Central Neurons, Controlling Wakefulness [Elektronische Daten] [Olga Sergeeva, Bettina Amberger, Helmut Haas] (1) Pre-mRNA editing of serotonin 2C (5-HT2C) and glutamate (Glu) receptors (R) influences higher brain functions and pathological states such as epilepsy, amyotrophic lateral sclerosis, and depression. Adenosine deaminases acting on RNA (ADAR1-3) convert adenosine to inosine on synthetic RNAs, analogous to 5-HT2cR and GluR. The order of editing as well as mechanisms controlling editing in native neurons is unknown. (2) With single-cell RT-PCR we investigated the co-expression of ADAR genes with GluR and 5-HT2CR and determined the editing status at known sites in the hypothalamic tuberomamillary nucleus, a major center for wakefulness and arousal. (3) The most frequently expressed enzymes were ADAR1, followed by ADAR2. The Q/R site of GluR2 was always fully edited. Editing at the R/G site in the GluR2 (but not GluR4) subunit was co-ordinated with ADAR expression: maximal editing was found in neurons expressing both ADAR2 splice variants of the deaminase domain and lacking ADAR3. (4) Editing of the 5-HT2CR did not correlate with ADAR expression. The 5-HT2CR mRNA was always edited at A, in the majority of cells at B sites and variably edited at E, C and D sites. A negative correlation was found between editing of C and D sites. The GluR4 R/G site editing was homogeneous within individuals: it was fully edited in all neurons obtained from 12 rats and under-edited in six neurons obtained from three rats. (5) We conclude that GluR2 R/G editing is controlled at the level of ADAR2 and therefore this enzyme may be a target for pharmacotherapy. On the other hand, further factors/enzymes besides ADAR must control or influence 5-HT2CR and GluR pre-mRNA editing in native neurons; our data indicate that these factors vary between individuals and could be predictors of psychiatric disease. Springer Science+Business Media, LLC, 2007 Adenosine deaminase acting at RNA nationallicence Serotonin nationallicence Glutamate nationallicence Histamine nationallicence Single-cell RT-PCR nationallicence Sergeeva Olga Department of Neurophysiology, Heinrich-Heine-Universität, POB 101007, 40001, Dusseldorf, Germany aut Amberger Bettina Department of Neurophysiology, Heinrich-Heine-Universität, POB 101007, 40001, Dusseldorf, Germany aut Haas Helmut Department of Neurophysiology, Heinrich-Heine-Universität, POB 101007, 40001, Dusseldorf, Germany aut Cellular and Molecular Neurobiology Kluwer Academic Publishers-Plenum Publishers; http://www.springer-ny.com 27/5(2007-07-01), 669-680 0272-4340 27:5<669 2007 27 10571 https://doi.org/10.1007/s10571-007-9153-1 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10571-007-9153-1 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Sergeeva Olga Department of Neurophysiology, Heinrich-Heine-Universität, POB 101007, 40001, Dusseldorf, Germany aut NATIONALLICENCE 700 1- Amberger Bettina Department of Neurophysiology, Heinrich-Heine-Universität, POB 101007, 40001, Dusseldorf, Germany aut NATIONALLICENCE 700 1- Haas Helmut Department of Neurophysiology, Heinrich-Heine-Universität, POB 101007, 40001, Dusseldorf, Germany aut NATIONALLICENCE 773 0- Cellular and Molecular Neurobiology Kluwer Academic Publishers-Plenum Publishers; http://www.springer-ny.com 27/5(2007-07-01), 669-680 0272-4340 27:5<669 2007 27 10571 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer