caa a22 4500 463254775 CHVBK 20180405153351.0 cr unu---uuuuu 170326e20070701xx s 000 0 eng 10.1007/s10571-007-9145-1 doi (NATIONALLICENCE)springer-10.1007/s10571-007-9145-1 Rapid Activation of the Extracellular Signal-Regulated Kinase 1/2 (ERK1/2) Signaling Pathway by Electroconvulsive Shock in the Rat Prefrontal Cortex Is Not Associated with TrkB Neurotrophin Receptor Activation [Elektronische Daten] [Henrik Hansen, Tomi Rantamäki, Marianne Larsen, David Woldbye, Jens Mikkelsen, Eero Castrén] 1. Emerging evidence indicates that brain-derived neurotrophic factor (BDNF) and its receptor TrkB play important roles in the mechanism of action of electroconvulsive shock (ECS) treatment. ECS produces a significant increase in brain BDNF synthesis together with a variety of neuroplastic changes including neurogenesis and axonal sprouting in the rodent brain, which is believed to be associated to the antidepressant effect of ECS. ERK1/2 (extracellular signal-regulated kinase-1/2) and Akt (protein kinase B), both intracellular signaling molecules being linked to neurotrophin signaling and synthesis, are important pathways triggered by TrkB autophosphorylation. 2. We have previously observed that chemical antidepressants induce a rapid activation of TrkB signaling in the rodent prefrontal cortex (PFC), which is likely a consequence of the stimulatory effect of antidepressants on BDNF synthesis. However, it is not known whether ECS triggers TrkB autophosphorylation and if any ECS-induced effect on TrkB function may be associated with the activation of the ERK1/2 and Akt pathways. 3. The present study assayed the phosphorylation levels of TrkB, ERK1/2, and Akt in the PFC of sham and ECS-treated rats. While the TrkB autophosphorylation (pTrkB) levels were decreased 30min after both acute and chronic ECS, no change in pTrkB levels were observed at any other time points measured. In contrast, acute but not chronic ECS, transiently induced a very rapid and robust hyperphosphorylation of ERK1/2. Akt phosphorylation levels remained unchanged following acute or chronic ECS. Hence, although ECS effectively stimulates the ERK1/2 pathway in the PFC, this effect does not appear to involve upstream activation of TrkB. Springer Science+Business Media, LLC, 2007 depression nationallicence electroconvulsive treatment nationallicence TrkB phosphorylation nationallicence BDNF nationallicence antidepressant nationallicence imipramine nationallicence Hansen Henrik Department of Translational Neurobiology, Neurosearch A/S, Pederstrupvej 93, DK-2750, Ballerup, Copenhagen, Denmark aut Rantamäki Tomi Neuroscience Center, University of Helsinki, (Viikinkaari 4), P.O.Box 56, Helsinki, Finland aut Larsen Marianne Department of Translational Neurobiology, Neurosearch A/S, Pederstrupvej 93, DK-2750, Ballerup, Copenhagen, Denmark aut Woldbye David Laboratory of Neuropsychiatry, Rigshospitalet University Hospital and Department of Neuroscience and Pharmacology, University of Copenhagen, Copenhagen, Denmark aut Mikkelsen Jens Department of Translational Neurobiology, Neurosearch A/S, Pederstrupvej 93, DK-2750, Ballerup, Copenhagen, Denmark aut Castrén Eero Neuroscience Center, University of Helsinki, (Viikinkaari 4), P.O.Box 56, Helsinki, Finland aut Cellular and Molecular Neurobiology Kluwer Academic Publishers-Plenum Publishers; http://www.springer-ny.com 27/5(2007-07-01), 585-594 0272-4340 27:5<585 2007 27 10571 https://doi.org/10.1007/s10571-007-9145-1 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10571-007-9145-1 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Hansen Henrik Department of Translational Neurobiology, Neurosearch A/S, Pederstrupvej 93, DK-2750, Ballerup, Copenhagen, Denmark aut NATIONALLICENCE 700 1- Rantamäki Tomi Neuroscience Center, University of Helsinki, (Viikinkaari 4), P.O.Box 56, Helsinki, Finland aut NATIONALLICENCE 700 1- Larsen Marianne Department of Translational Neurobiology, Neurosearch A/S, Pederstrupvej 93, DK-2750, Ballerup, Copenhagen, Denmark aut NATIONALLICENCE 700 1- Woldbye David Laboratory of Neuropsychiatry, Rigshospitalet University Hospital and Department of Neuroscience and Pharmacology, University of Copenhagen, Copenhagen, Denmark aut NATIONALLICENCE 700 1- Mikkelsen Jens Department of Translational Neurobiology, Neurosearch A/S, Pederstrupvej 93, DK-2750, Ballerup, Copenhagen, Denmark aut NATIONALLICENCE 700 1- Castrén Eero Neuroscience Center, University of Helsinki, (Viikinkaari 4), P.O.Box 56, Helsinki, Finland aut NATIONALLICENCE 773 0- Cellular and Molecular Neurobiology Kluwer Academic Publishers-Plenum Publishers; http://www.springer-ny.com 27/5(2007-07-01), 585-594 0272-4340 27:5<585 2007 27 10571 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer