caa a22 4500 463254791 CHVBK 20180405153351.0 cr unu---uuuuu 170326e20070701xx s 000 0 eng 10.1007/s10571-007-9144-2 doi (NATIONALLICENCE)springer-10.1007/s10571-007-9144-2 Time Course of c-fos , vasopressin and oxytocin mRNA Expression in the Hypothalamus Following Long-Term Dehydration [Elektronische Daten] [Lenise da Silveira, Cristina Junta, Nadia Monesi, Gabriela de Oliveira-Pelegrin, Geraldo Passos, Maria Rocha] Summary: 1. This study presents a time course analysis of the messenger RNA (mRNA) levels of c-fos, vasopressin (VP), and oxytocin (OT) in the paraventricular (PVN) and supraoptic nucleus (SON), following acute and chronic dehydration by water deprivation. 2. Male Wistar rats were separated into five groups: nondehydrated (control group) and dehydrated for 6, 24, 48 and 72h. Following water deprivation, animals were decapitated, their blood was collected for hematocrit, osmolality, and plasma sodium measurements, and brains were removed for dissection of both PVN and SON. 3. As expected, the hematocrit, osmolality, plasma sodium, and weight loss were increased after water deprivation. In SON, a significant increase in both VP and OT mRNA expression was observed 6h after dehydration reaching a peak at 24h and returning to basal levels of expression at 72h. In the PVN, an increase in both VP and OT mRNA expression occurred 24h after dehydration. At 72h the VP and OT mRNA expression levels had decreased but they were still at higher levels than those detected in control animals. 4. These results suggest that SON is the first nucleus to respond to the dehydration stimulus. Additionally, we also observed an increase in c-fos mRNA expression in both PVN and SON 6h after water deprivation, which progressively decreased 24, 48, and 72h after the onset of water deprivation. Therefore, it is possible that c-fos may be involved in the modulation of VP and OT genes, regulating the mRNA expression levels on a temporally distinct basis within the PVN and SON. Springer Science+Business Media, LLC, 2007 water deprivation nationallicence neurohypophysial hormones nationallicence fos protein nationallicence paraventricular nucleus nationallicence supraoptic nucleus nationallicence VP and OT mRNA expression nationallicence da Silveira Lenise Departamento de Análises Clínicas, Toxicológicas e Bromatológicas da Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Ribeirão Preto, Brasil aut Junta Cristina Departamento de Genética da Faculdade de Medicina de Ribeirão Preto, Ribeirão Preto, Brasil aut Monesi Nadia Departamento de Análises Clínicas, Toxicológicas e Bromatológicas da Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Ribeirão Preto, Brasil aut de Oliveira-Pelegrin Gabriela Departamento de Análises Clínicas, Toxicológicas e Bromatológicas da Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Ribeirão Preto, Brasil aut Passos Geraldo Departamento de Genética da Faculdade de Medicina de Ribeirão Preto, Ribeirão Preto, Brasil aut Rocha Maria Departamento de Morfologia, Estomatologia e Fisiologia da Faculdade de Odontologia de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, SP, Brasil aut Cellular and Molecular Neurobiology Kluwer Academic Publishers-Plenum Publishers; http://www.springer-ny.com 27/5(2007-07-01), 575-584 0272-4340 27:5<575 2007 27 10571 https://doi.org/10.1007/s10571-007-9144-2 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10571-007-9144-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- da Silveira Lenise Departamento de Análises Clínicas, Toxicológicas e Bromatológicas da Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Ribeirão Preto, Brasil aut NATIONALLICENCE 700 1- Junta Cristina Departamento de Genética da Faculdade de Medicina de Ribeirão Preto, Ribeirão Preto, Brasil aut NATIONALLICENCE 700 1- Monesi Nadia Departamento de Análises Clínicas, Toxicológicas e Bromatológicas da Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Ribeirão Preto, Brasil aut NATIONALLICENCE 700 1- de Oliveira-Pelegrin Gabriela Departamento de Análises Clínicas, Toxicológicas e Bromatológicas da Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Ribeirão Preto, Brasil aut NATIONALLICENCE 700 1- Passos Geraldo Departamento de Genética da Faculdade de Medicina de Ribeirão Preto, Ribeirão Preto, Brasil aut NATIONALLICENCE 700 1- Rocha Maria Departamento de Morfologia, Estomatologia e Fisiologia da Faculdade de Odontologia de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, SP, Brasil aut NATIONALLICENCE 773 0- Cellular and Molecular Neurobiology Kluwer Academic Publishers-Plenum Publishers; http://www.springer-ny.com 27/5(2007-07-01), 575-584 0272-4340 27:5<575 2007 27 10571 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer