caa a22 4500 463254821 CHVBK 20180405153351.0 cr unu---uuuuu 170326e20070201xx s 000 0 eng 10.1007/s10571-006-9104-2 doi (NATIONALLICENCE)springer-10.1007/s10571-006-9104-2 Postischemic Alterations of BDNF, NGF, HSP 70 and Ubiquitin Immunoreactivity in the Gerbil Hippocampus: Pharmacological Approach [Elektronische Daten] [Toshiki Himeda, Hiroko Tounai, Natsumi Hayakawa, Tsutomu Araki] 1. We investigated the immunohistochemical alterations of BDNF, NGF, HSP 70 and ubiquitin in the hippocampus 1h to 14 days after transient cerebral ischemia in gerbils. We also examined the effect of 3-hydroxy-3-methylglutaryl-coenzyme A (HMG-CoA) reductase inhibitor pitavastatin against the changes of BDNF, NGF, HSP 70 and ubiquitin in the hippocampus after cerebral ischemia in the hippocampus after ischemia. 2. The transient cerebral ischemia was carried out by clamping the carotid arteries with aneurismal clips for 5min. 3. In the present study, the alteration of HSP 70 and ubiquitin immunoreactivity in the hippocampal CA1 sector was more pronounced than that of BDNF and NGF immunoreactivity after transient cerebral ischemia. In double-labeled immunostainings, BDNF, NGF and ubiquitin immunostaining was observed both in GFAP-positive astrocytes and MRF-1-positive microglia in the hippocampal CA1 sector after ischemia. Furthermore, prophylactic treatment with pitavastatin prevented the damage of neurons with neurotrophic factor and stress proteins in the hippocampal CA1 sector after ischemia. 4. These findings suggest that the expression of stress protein including HSP 70 and ubiquitin may play a key role in the protection against the hippocampal CA1 neuronal damage after transient cerebral ischemia in comparison with the expression of neurotrophic factor such as BDNF and NGF. The present findings also suggest that the glial BDNF, NGF and ubiquitin may play some role for helping surviving neurons after ischemia. Furthermore, our present study indicates that prophylactic treatment with pitavastatin can prevent the damage of neurons with neurotrophic factor and stress proteins in the hippocampal CA1 sector after transient cerebral ischemia. Thus our study provides further valuable information for the pathogenesis after transient cerebral ischemia. Springer Science+Business Media, Inc., 2006 cerebral ischemia nationallicence neurotrophic factor nationallicence stress protein nationallicence gerbil nationallicence 3-Hydroxy-3-methylglutaryl-coenzyme a reductase inhibitor nationallicence Himeda Toshiki Department of Drug Metabolism and Therapeutics, Graduate school and Faculty of Pharmaceutical Sciences, The University of Tokushima, 1-78 Sho-machi, 770-8505, Tokushima, Japan aut Tounai Hiroko Department of Drug Metabolism and Therapeutics, Graduate school and Faculty of Pharmaceutical Sciences, The University of Tokushima, 1-78 Sho-machi, 770-8505, Tokushima, Japan aut Hayakawa Natsumi Department of Drug Metabolism and Therapeutics, Graduate school and Faculty of Pharmaceutical Sciences, The University of Tokushima, 1-78 Sho-machi, 770-8505, Tokushima, Japan aut Araki Tsutomu Department of Drug Metabolism and Therapeutics, Graduate school and Faculty of Pharmaceutical Sciences, The University of Tokushima, 1-78 Sho-machi, 770-8505, Tokushima, Japan aut Cellular and Molecular Neurobiology Kluwer Academic Publishers-Plenum Publishers; http://www.springer-ny.com 27/2(2007-02-01), 229-250 0272-4340 27:2<229 2007 27 10571 https://doi.org/10.1007/s10571-006-9104-2 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10571-006-9104-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Himeda Toshiki Department of Drug Metabolism and Therapeutics, Graduate school and Faculty of Pharmaceutical Sciences, The University of Tokushima, 1-78 Sho-machi, 770-8505, Tokushima, Japan aut NATIONALLICENCE 700 1- Tounai Hiroko Department of Drug Metabolism and Therapeutics, Graduate school and Faculty of Pharmaceutical Sciences, The University of Tokushima, 1-78 Sho-machi, 770-8505, Tokushima, Japan aut NATIONALLICENCE 700 1- Hayakawa Natsumi Department of Drug Metabolism and Therapeutics, Graduate school and Faculty of Pharmaceutical Sciences, The University of Tokushima, 1-78 Sho-machi, 770-8505, Tokushima, Japan aut NATIONALLICENCE 700 1- Araki Tsutomu Department of Drug Metabolism and Therapeutics, Graduate school and Faculty of Pharmaceutical Sciences, The University of Tokushima, 1-78 Sho-machi, 770-8505, Tokushima, Japan aut NATIONALLICENCE 773 0- Cellular and Molecular Neurobiology Kluwer Academic Publishers-Plenum Publishers; http://www.springer-ny.com 27/2(2007-02-01), 229-250 0272-4340 27:2<229 2007 27 10571 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer