caa a22 4500 463255127 CHVBK 20180405153352.0 cr unu---uuuuu 170326e20071001xx s 000 0 eng 10.1007/s00435-007-0035-3 doi (NATIONALLICENCE)springer-10.1007/s00435-007-0035-3 Gill-symbiosis in mytilidae associated with wood fall environments [Elektronische Daten] [Olivier Gros, Julien Guibert, Françoise Gaill] Bivalves belonging to the genera Idas and Adipicola were collected from wood fall environments in the west Pacific (Vanuatu islands) between 300 and 890m depths in 2004. Bacterial symbionts were checked by three complementary techniques: histological and DAPI staining, in situ hybridization (FISH), and TEM. No bacteria were detected inside the gills of the two species, rejecting the endosymbiosis hypothesis. However, results from our study demonstrated the existence of ectosymbionts colonizing microvilli differentiated at the apical surface of the cells constituting the lateral zone of gill filaments. These ectosymbionts are γ-Proteobacteria due to their strong hybridization with the specific probe GAM42; in contrast no hybridization was obtained from either gills or other host tissues by using the oligonucleotide probes specific to α- β- and δ-Proteobacteria. Based on TEM observations, these Gram-negative bacterial symbionts are not methanotrophic due to the lack of concentric stacking of intracellular membranes in their cytoplasm. Such ectosymbionts may represent thioautotrophic bacteria as already described in various Mytilidae from hydrothermal vents and cold seeps. Unfortunately, no phylogenetic analysis could be done in this study to compare their DNA sequence to that of other marine invertebrate symbionts described to date. Springer-Verlag, 2007 Ectosymbiosis nationallicence Mytilidae nationallicence Wood fall nationallicence Ultrastructure nationallicence Gros Olivier UMR-CNRS 7138, Systématique-Adaptation-Evolution, Equipe "Symbiose”, UFR des Sciences Exactes et Naturelles, Département de Biologie, Université des Antilles et de la Guyane, B.P. 592, 97159, Pointe-à-Pitre Cedex, Guadeloupe, France aut Guibert Julien UMR-CNRS 7138, Systématique-Adaptation-Evolution, Equipe "Symbiose”, UFR des Sciences Exactes et Naturelles, Département de Biologie, Université des Antilles et de la Guyane, B.P. 592, 97159, Pointe-à-Pitre Cedex, Guadeloupe, France aut Gaill Françoise UMR-CNRS 7138, Systématique-Adaptation-Evolution, Equipe "Adaptation et Evolution en milieux extremes”, Université Pierre et Marie Curie, Bat A 4eme étage, case 5. 7 Quai Saint Bernard, 75005, Paris, France aut Zoomorphology Springer-Verlag 126/3(2007-10-01), 163-172 0720-213X 126:3<163 2007 126 435 https://doi.org/10.1007/s00435-007-0035-3 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00435-007-0035-3 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Gros Olivier UMR-CNRS 7138, Systématique-Adaptation-Evolution, Equipe "Symbiose”, UFR des Sciences Exactes et Naturelles, Département de Biologie, Université des Antilles et de la Guyane, B.P. 592, 97159, Pointe-à-Pitre Cedex, Guadeloupe, France aut NATIONALLICENCE 700 1- Guibert Julien UMR-CNRS 7138, Systématique-Adaptation-Evolution, Equipe "Symbiose”, UFR des Sciences Exactes et Naturelles, Département de Biologie, Université des Antilles et de la Guyane, B.P. 592, 97159, Pointe-à-Pitre Cedex, Guadeloupe, France aut NATIONALLICENCE 700 1- Gaill Françoise UMR-CNRS 7138, Systématique-Adaptation-Evolution, Equipe "Adaptation et Evolution en milieux extremes”, Université Pierre et Marie Curie, Bat A 4eme étage, case 5. 7 Quai Saint Bernard, 75005, Paris, France aut NATIONALLICENCE 773 0- Zoomorphology Springer-Verlag 126/3(2007-10-01), 163-172 0720-213X 126:3<163 2007 126 435 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer