caa a22 4500 46325533X CHVBK 20180405153353.0 cr unu---uuuuu 170326e20070101xx s 000 0 eng 10.1007/s00277-006-0166-5 doi (NATIONALLICENCE)springer-10.1007/s00277-006-0166-5 Human mesenchymal stem cells favour healing of the cutaneous radiation syndrome in a xenogenic transplant model [Elektronische Daten] [Sabine François, Moubarak Mouiseddine, Noëlle Mathieu, Alexandra Semont, Pascale Monti, Nicolas Dudoignon, Amandine Saché, Asma Boutarfa, Dominique Thierry, Patrick Gourmelon, Alain Chapel] It has been suggested that human mesenchymal stem cells (hMSC) could be used to repair numerous injured tissues. We have studied the potential use of hMSC to limit radiation-induced skin lesions. Immunodeficient NOD/SCID mice were locally irradiated to the leg (30Gy, dose rate 2.7Gy/min) using a 60Co source to induce a severe skin lesion. Cultured bone marrow hMSC were delivered intravenously to the mice. The irradiated skin samples were studied for the presence of the human cells, the severity of the lesions and the healing process. Macroscopic analysis and histology results showed that the lesions were evolving to a less severe degree of radiation dermatitis after hMSC transplant when compared to irradiated non-transplanted controls. Clinical scores for the studied skin parameters of treated mice were significantly improved. A faster healing was observed when compared to untreated mouse. Immunohistology and polymerase chain reaction analysis provided evidence that the human cells were found in the irradiated area. These results suggest a possible use of hMSC for the treatment of the early phase of the cutaneous radiation syndrome. A successful transplant of stem cells and subsequent reduction in radiation-induced complication may open the road to completely new strategies in cutaneous radiation syndrome therapy. Springer-Verlag, 2006 Human mesenchymal stem cells nationallicence Radiation-induced skin lesions nationallicence Cutaneous radiation syndrome nationallicence Xenogenic transplant model nationallicence François Sabine Laboratory of Cell Therapy and Radioprotection of the Accident, Radiobiology and Epidemiology Department, Radiological Protection and Human Health Division, Institut de Radioprotection et de Sûreté Nucléaire DRPH/SRBE/LTCRA, IRSN BP no. 17, 92262, Fontenay aux Roses CEDEX, France aut Mouiseddine Moubarak Laboratory of Cell Therapy and Radioprotection of the Accident, Radiobiology and Epidemiology Department, Radiological Protection and Human Health Division, Institut de Radioprotection et de Sûreté Nucléaire DRPH/SRBE/LTCRA, IRSN BP no. 17, 92262, Fontenay aux Roses CEDEX, France aut Mathieu Noëlle Laboratory of Cell Therapy and Radioprotection of the Accident, Radiobiology and Epidemiology Department, Radiological Protection and Human Health Division, Institut de Radioprotection et de Sûreté Nucléaire DRPH/SRBE/LTCRA, IRSN BP no. 17, 92262, Fontenay aux Roses CEDEX, France aut Semont Alexandra Laboratory of Cell Therapy and Radioprotection of the Accident, Radiobiology and Epidemiology Department, Radiological Protection and Human Health Division, Institut de Radioprotection et de Sûreté Nucléaire DRPH/SRBE/LTCRA, IRSN BP no. 17, 92262, Fontenay aux Roses CEDEX, France aut Monti Pascale Laboratory of Cell Therapy and Radioprotection of the Accident, Radiobiology and Epidemiology Department, Radiological Protection and Human Health Division, Institut de Radioprotection et de Sûreté Nucléaire DRPH/SRBE/LTCRA, IRSN BP no. 17, 92262, Fontenay aux Roses CEDEX, France aut Dudoignon Nicolas Laboratory of Radiopathology Radiobiology and Epidemiology Department, Radiological Protection and Human Health Division, Institut de Radioprotection et de Sûreté Nucléaire DRPH/SRBE/LTCRA, IRSN BP no. 17, 92262, Fontenay aux Roses CEDEX, France aut Saché Amandine Laboratory of Cell Therapy and Radioprotection of the Accident, Radiobiology and Epidemiology Department, Radiological Protection and Human Health Division, Institut de Radioprotection et de Sûreté Nucléaire DRPH/SRBE/LTCRA, IRSN BP no. 17, 92262, Fontenay aux Roses CEDEX, France aut Boutarfa Asma Laboratory of Cell Therapy and Radioprotection of the Accident, Radiobiology and Epidemiology Department, Radiological Protection and Human Health Division, Institut de Radioprotection et de Sûreté Nucléaire DRPH/SRBE/LTCRA, IRSN BP no. 17, 92262, Fontenay aux Roses CEDEX, France aut Thierry Dominique Laboratory of Cell Therapy and Radioprotection of the Accident, Radiobiology and Epidemiology Department, Radiological Protection and Human Health Division, Institut de Radioprotection et de Sûreté Nucléaire DRPH/SRBE/LTCRA, IRSN BP no. 17, 92262, Fontenay aux Roses CEDEX, France aut Gourmelon Patrick Radiological Protection and Human Health Division, Institut de Radioprotection et de Sûreté Nucléaire (IRSN/DRPH), BP no. 17, 92262, Fontenay aux Roses CEDEX, France aut Chapel Alain Laboratory of Cell Therapy and Radioprotection of the Accident, Radiobiology and Epidemiology Department, Radiological Protection and Human Health Division, Institut de Radioprotection et de Sûreté Nucléaire DRPH/SRBE/LTCRA, IRSN BP no. 17, 92262, Fontenay aux Roses CEDEX, France aut Annals of Hematology Springer-Verlag 86/1(2007-01-01), 1-8 0939-5555 86:1<1 2007 86 277 https://doi.org/10.1007/s00277-006-0166-5 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00277-006-0166-5 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- François Sabine Laboratory of Cell Therapy and Radioprotection of the Accident, Radiobiology and Epidemiology Department, Radiological Protection and Human Health Division, Institut de Radioprotection et de Sûreté Nucléaire DRPH/SRBE/LTCRA, IRSN BP no. 17, 92262, Fontenay aux Roses CEDEX, France aut NATIONALLICENCE 700 1- Mouiseddine Moubarak Laboratory of Cell Therapy and Radioprotection of the Accident, Radiobiology and Epidemiology Department, Radiological Protection and Human Health Division, Institut de Radioprotection et de Sûreté Nucléaire DRPH/SRBE/LTCRA, IRSN BP no. 17, 92262, Fontenay aux Roses CEDEX, France aut NATIONALLICENCE 700 1- Mathieu Noëlle Laboratory of Cell Therapy and Radioprotection of the Accident, Radiobiology and Epidemiology Department, Radiological Protection and Human Health Division, Institut de Radioprotection et de Sûreté Nucléaire DRPH/SRBE/LTCRA, IRSN BP no. 17, 92262, Fontenay aux Roses CEDEX, France aut NATIONALLICENCE 700 1- Semont Alexandra Laboratory of Cell Therapy and Radioprotection of the Accident, Radiobiology and Epidemiology Department, Radiological Protection and Human Health Division, Institut de Radioprotection et de Sûreté Nucléaire DRPH/SRBE/LTCRA, IRSN BP no. 17, 92262, Fontenay aux Roses CEDEX, France aut NATIONALLICENCE 700 1- Monti Pascale Laboratory of Cell Therapy and Radioprotection of the Accident, Radiobiology and Epidemiology Department, Radiological Protection and Human Health Division, Institut de Radioprotection et de Sûreté Nucléaire DRPH/SRBE/LTCRA, IRSN BP no. 17, 92262, Fontenay aux Roses CEDEX, France aut NATIONALLICENCE 700 1- Dudoignon Nicolas Laboratory of Radiopathology Radiobiology and Epidemiology Department, Radiological Protection and Human Health Division, Institut de Radioprotection et de Sûreté Nucléaire DRPH/SRBE/LTCRA, IRSN BP no. 17, 92262, Fontenay aux Roses CEDEX, France aut NATIONALLICENCE 700 1- Saché Amandine Laboratory of Cell Therapy and Radioprotection of the Accident, Radiobiology and Epidemiology Department, Radiological Protection and Human Health Division, Institut de Radioprotection et de Sûreté Nucléaire DRPH/SRBE/LTCRA, IRSN BP no. 17, 92262, Fontenay aux Roses CEDEX, France aut NATIONALLICENCE 700 1- Boutarfa Asma Laboratory of Cell Therapy and Radioprotection of the Accident, Radiobiology and Epidemiology Department, Radiological Protection and Human Health Division, Institut de Radioprotection et de Sûreté Nucléaire DRPH/SRBE/LTCRA, IRSN BP no. 17, 92262, Fontenay aux Roses CEDEX, France aut NATIONALLICENCE 700 1- Thierry Dominique Laboratory of Cell Therapy and Radioprotection of the Accident, Radiobiology and Epidemiology Department, Radiological Protection and Human Health Division, Institut de Radioprotection et de Sûreté Nucléaire DRPH/SRBE/LTCRA, IRSN BP no. 17, 92262, Fontenay aux Roses CEDEX, France aut NATIONALLICENCE 700 1- Gourmelon Patrick Radiological Protection and Human Health Division, Institut de Radioprotection et de Sûreté Nucléaire (IRSN/DRPH), BP no. 17, 92262, Fontenay aux Roses CEDEX, France aut NATIONALLICENCE 700 1- Chapel Alain Laboratory of Cell Therapy and Radioprotection of the Accident, Radiobiology and Epidemiology Department, Radiological Protection and Human Health Division, Institut de Radioprotection et de Sûreté Nucléaire DRPH/SRBE/LTCRA, IRSN BP no. 17, 92262, Fontenay aux Roses CEDEX, France aut NATIONALLICENCE 773 0- Annals of Hematology Springer-Verlag 86/1(2007-01-01), 1-8 0939-5555 86:1<1 2007 86 277 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer