caa a22 4500 463255925 CHVBK 20180405153355.0 cr unu---uuuuu 170326e20071201xx s 000 0 eng 10.1007/s00277-007-0352-0 doi (NATIONALLICENCE)springer-10.1007/s00277-007-0352-0 Signaling status of IgG B cell receptor (IgG BCR) is indicative for an activated state of circulating B cells in multiple myeloma [Elektronische Daten] [Vesna Ilić, Nadežda Milošević-Jovčić, Sonja Petrović, Dragana Marković, Jelena Bila, Darinka Bošković, Gordana Stefanović, Olivera Marković, Marija Glibetić] Circulating post-switch B cells have been proposed as proliferative and disseminating progenitors in multiple myeloma. It is unclear whether the class-switched antigen receptor expressed at the surface of these cells plays a role in their expansion. In this work, the signaling status of IgG B cell receptor (BCR) isolated from the lysates of peripheral blood lymphocytes of 32 patients with IgG multiple myeloma, at the time of diagnosis, was investigated by examining whether phosphorylation of BCR Igα and Igβ signal transducer factors (co-receptors) or other signaling molecules was abnormal in these cells when compared with healthy controls. In IgG BCR of normal controls, weak phosphorylation of 56 and 61kDa Src kinase-related proteins and unphosphorylated co-receptors were found. In myeloma, p56 and p61kDa proteins, co-receptors, and other IgG BCR-associated proteins from the signal cascade were phosphorylated. Myeloma patients can be classified into subgroups by IgG BCR phosphorylation profiles which characteristically coordinated with the level of IgG paraprotein in serum and the stage of disease. There was a correlative trend between the extent of phosphorylation reduction and advanced stage of disease. Reduced phosphorylation was more pronounced with advanced stages of multiple myeloma. Springer-Verlag, 2007 IgG B-cell receptor nationallicence Tyrosine phosphorylation nationallicence Multiple myeloma nationallicence Ilić Vesna Institute for Medical Research, University of Belgrade, Belgrade, Serbia aut Milošević-Jovčić Nadežda Institute for Medical Research, University of Belgrade, Belgrade, Serbia aut Petrović Sonja Institute for Medical Research, University of Belgrade, Belgrade, Serbia aut Marković Dragana Institute for Medical Research, University of Belgrade, Belgrade, Serbia aut Bila Jelena Institute for Hematology, School of Medicine, University of Belgrade, Belgrade, Serbia aut Bošković Darinka Institute for Hematology, School of Medicine, University of Belgrade, Belgrade, Serbia aut Stefanović Gordana School of Dentistry, Department of Physiology, University of Belgrade, Belgrade, Serbia aut Marković Olivera Department of Hematology, Clinico-Hospital Center "Bežanijska Kosa”, Belgrade, Serbia aut Glibetić Marija Institute for Medical Research, University of Belgrade, Belgrade, Serbia aut Annals of Hematology Springer-Verlag 86/12(2007-12-01), 905-912 0939-5555 86:12<905 2007 86 277 https://doi.org/10.1007/s00277-007-0352-0 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00277-007-0352-0 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Ilić Vesna Institute for Medical Research, University of Belgrade, Belgrade, Serbia aut NATIONALLICENCE 700 1- Milošević-Jovčić Nadežda Institute for Medical Research, University of Belgrade, Belgrade, Serbia aut NATIONALLICENCE 700 1- Petrović Sonja Institute for Medical Research, University of Belgrade, Belgrade, Serbia aut NATIONALLICENCE 700 1- Marković Dragana Institute for Medical Research, University of Belgrade, Belgrade, Serbia aut NATIONALLICENCE 700 1- Bila Jelena Institute for Hematology, School of Medicine, University of Belgrade, Belgrade, Serbia aut NATIONALLICENCE 700 1- Bošković Darinka Institute for Hematology, School of Medicine, University of Belgrade, Belgrade, Serbia aut NATIONALLICENCE 700 1- Stefanović Gordana School of Dentistry, Department of Physiology, University of Belgrade, Belgrade, Serbia aut NATIONALLICENCE 700 1- Marković Olivera Department of Hematology, Clinico-Hospital Center "Bežanijska Kosa”, Belgrade, Serbia aut NATIONALLICENCE 700 1- Glibetić Marija Institute for Medical Research, University of Belgrade, Belgrade, Serbia aut NATIONALLICENCE 773 0- Annals of Hematology Springer-Verlag 86/12(2007-12-01), 905-912 0939-5555 86:12<905 2007 86 277 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer