caa a22 4500 463255984 CHVBK 20180405153355.0 cr unu---uuuuu 170326e20071201xx s 000 0 eng 10.1007/s00277-007-0354-y doi (NATIONALLICENCE)springer-10.1007/s00277-007-0354-y Inhibition of adrenaline and adenosine diphosphate induced platelet aggregation by Lansberg's hognose pit viper ( Porthidium lansbergii hutmanni ) venom [Elektronische Daten] [J. Lopez-Johnston, N. de Bosch, H. Scannone, A. Rodríguez-Acosta] The haemostatic components of venom from the genus Porthidium has been poorly studied, although it is known that severe manifestations occur when humans are envenomed, which include invasive oedema and disseminated ecchymosis. The effects of venom on blood platelets are commonly studied and are normally carried out with platelet-rich plasma (PRP). A series of crude venom dilutions was used to determine the effects of adenosine diphosphate (2μM) and adrenaline (11μM) induced platelet aggregation. Venom of Porthidium lansbergii hutmanni was fractioned by anionic exchange chromatography, and the fractions were also used to determine the 50% inhibition of adenosine diphosphate (ADP) and adrenaline-induced platelet aggregating dose (AD50). Crude venom has more effect in inhibiting adrenaline-induced aggregation (AD50 = 0.0043μg) followed by the adenosine diphosphate (AD50 = 17μg). Peaks I and II obtained by chromatography also inhibited adrenaline-induced platelet aggregation with an AD50 of 3.2 and 0.013μg, respectively, and both peaks inhibited ADP-induced platelet aggregation with an AD50 of 10μg. The main purpose of this work was to characterise the in vitro effects caused by P. lansbergii hutmanni crude venom and its fractions on the platelet aggregation mediated by adrenaline and ADP agonists. Springer-Verlag, 2007 Adenosine diphosphate nationallicence Adrenaline nationallicence Platelet aggregation nationallicence Porthidium lansbergii hutmanni nationallicence Snake venom nationallicence Lopez-Johnston J. Laboratorio de Investigaciones, Facultad de Farmacia, Universidad Central de Venezuela, Caracas, Venezuela aut de Bosch N. Banco Municipal de Sangre de Caracas, Caracas, Venezuela aut Scannone H. Laboratorio de Investigaciones, Facultad de Farmacia, Universidad Central de Venezuela, Caracas, Venezuela aut Rodríguez-Acosta A. Sección de Inmunoquímica, Instituto de Medicina Tropical, Universidad Central de Venezuela, Apartado 47423, 1041, Caracas, Venezuela aut Annals of Hematology Springer-Verlag 86/12(2007-12-01), 879-885 0939-5555 86:12<879 2007 86 277 https://doi.org/10.1007/s00277-007-0354-y text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00277-007-0354-y text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Lopez-Johnston J. Laboratorio de Investigaciones, Facultad de Farmacia, Universidad Central de Venezuela, Caracas, Venezuela aut NATIONALLICENCE 700 1- de Bosch N. Banco Municipal de Sangre de Caracas, Caracas, Venezuela aut NATIONALLICENCE 700 1- Scannone H. Laboratorio de Investigaciones, Facultad de Farmacia, Universidad Central de Venezuela, Caracas, Venezuela aut NATIONALLICENCE 700 1- Rodríguez-Acosta A. Sección de Inmunoquímica, Instituto de Medicina Tropical, Universidad Central de Venezuela, Apartado 47423, 1041, Caracas, Venezuela aut NATIONALLICENCE 773 0- Annals of Hematology Springer-Verlag 86/12(2007-12-01), 879-885 0939-5555 86:12<879 2007 86 277 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer