caa a22 4500 463256352 CHVBK 20180405153356.0 cr unu---uuuuu 170326e20070401xx s 000 0 eng 10.1007/s00277-006-0214-1 doi (NATIONALLICENCE)springer-10.1007/s00277-006-0214-1 Biomarker analysis in polycythemia vera under interferon-alpha treatment: clonality, EEC, PRV-1, and JAK2 V617F [Elektronische Daten] [C. Steimle, U. Lehmann, S. Temerinac, Ph. Goerttler, H. Kreipe, G. Meinhardt, H. Heimpel, H. Pahl] Three consecutive polycythemia vera (PV) patients were analyzed before and during pegylated-interferon (rIFNα) treatment for the following markers: (1) granulocyte and CD34+ cell clonality, (2) Jak2V617F expression, (3) PRV-1 mRNA overexpression, and (4) Epo-independent colony (EEC) growth. Before rIFNα therapy, all patients displayed clonal hematopoiesis, 100% Jak2V617F expression as well as PRV-1 overexpression, and EEC growth. After rIFNα treatment, all three patients demonstrated polyclonal hematopoiesis. Nonetheless, Jak2V617F expression, PRV-1 overexpression, and EEC-growth remained detectable, albeit at lower levels. We conclude that reemergence of polyclonal hematopoiesis after rIFNα treatment may be achieved in a substantial proportion of patients. However, this does not constitute elimination of the PV clone. These data demonstrate the usefulness of novel markers in monitoring minimal residual disease and caution against discontinuation of rIFNα treatment after hematologic remission. Long-term follow-up of large patient cohorts is required to determine whether rIFNα treatment can cause complete molecular remissions in PV. Springer-Verlag, 2007 Polycythemia vera nationallicence Pegylated-interferon (rIFNα) treatment nationallicence Granulocyte and CD34+ cell clonality nationallicence Jak2V617F expression nationallicence PRV-1 mRNA overexpression nationallicence EEC growth nationallicence Steimle C. Department of Experimental Anaesthesiology, University Hospital Freiburg, Center for Clinical Research, Breisacher Str. 66, 79106, Freiburg, Germany aut Lehmann U. Institute of Pathology, Medizinische Hochschule Hannover, Carl-Neuberg-Str.1, 30625, Hannover, Germany aut Temerinac S. Department of Experimental Anaesthesiology, University Hospital Freiburg, Center for Clinical Research, Breisacher Str. 66, 79106, Freiburg, Germany aut Goerttler Ph Department of Experimental Anaesthesiology, University Hospital Freiburg, Center for Clinical Research, Breisacher Str. 66, 79106, Freiburg, Germany aut Kreipe H. Institute of Pathology, Medizinische Hochschule Hannover, Carl-Neuberg-Str.1, 30625, Hannover, Germany aut Meinhardt G. Department of Hematology/Oncology, Klinikum Innenstadt, Ludwig-Maximilians-Universität München, Ziemssenstr. 1, 80336, München, Germany aut Heimpel H. Department of Hematology and Oncology, University Hospital Ulm, Robert-Koch-Str. 5, 89081, Ulm, Germany aut Pahl H. Department of Experimental Anaesthesiology, University Hospital Freiburg, Center for Clinical Research, Breisacher Str. 66, 79106, Freiburg, Germany aut Annals of Hematology Springer-Verlag 86/4(2007-04-01), 239-244 0939-5555 86:4<239 2007 86 277 https://doi.org/10.1007/s00277-006-0214-1 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00277-006-0214-1 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Steimle C. Department of Experimental Anaesthesiology, University Hospital Freiburg, Center for Clinical Research, Breisacher Str. 66, 79106, Freiburg, Germany aut NATIONALLICENCE 700 1- Lehmann U. Institute of Pathology, Medizinische Hochschule Hannover, Carl-Neuberg-Str.1, 30625, Hannover, Germany aut NATIONALLICENCE 700 1- Temerinac S. Department of Experimental Anaesthesiology, University Hospital Freiburg, Center for Clinical Research, Breisacher Str. 66, 79106, Freiburg, Germany aut NATIONALLICENCE 700 1- Goerttler Ph Department of Experimental Anaesthesiology, University Hospital Freiburg, Center for Clinical Research, Breisacher Str. 66, 79106, Freiburg, Germany aut NATIONALLICENCE 700 1- Kreipe H. Institute of Pathology, Medizinische Hochschule Hannover, Carl-Neuberg-Str.1, 30625, Hannover, Germany aut NATIONALLICENCE 700 1- Meinhardt G. Department of Hematology/Oncology, Klinikum Innenstadt, Ludwig-Maximilians-Universität München, Ziemssenstr. 1, 80336, München, Germany aut NATIONALLICENCE 700 1- Heimpel H. Department of Hematology and Oncology, University Hospital Ulm, Robert-Koch-Str. 5, 89081, Ulm, Germany aut NATIONALLICENCE 700 1- Pahl H. Department of Experimental Anaesthesiology, University Hospital Freiburg, Center for Clinical Research, Breisacher Str. 66, 79106, Freiburg, Germany aut NATIONALLICENCE 773 0- Annals of Hematology Springer-Verlag 86/4(2007-04-01), 239-244 0939-5555 86:4<239 2007 86 277 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer