caa a22 4500 465731880 CHVBK 20180323111743.0 cr unu---uuuuu 170327e19900401xx s 000 0 eng 10.1007/BF02061805 doi (NATIONALLICENCE)springer-10.1007/BF02061805 Some aspects of optimization of separation system in radioimmunoassay of triiodothyronite (T3) [Elektronische Daten] [A. Rathinaswamy, U. Nagvekar, S. Borkute, S. Balakrishnan, C. Desai] The need for defining the assay conditions assumes greater significance for the manufacturer of RIA kits. This stems from the consideration that despite high quality of kit reagents in the kits, it is the definition of optimum conditions of assay that makes them effective and also permits to evaluate the limits of the sensitivity and precision. In this connection we have studied in detail a number of parameters mainly to define the stability of the assay system to changes in the protein concentration, the influence of polyethylene glycol in various ionic media, incubation parameters for systematic error analysis in three separation systems based on dextran coated charcoal, plain polyethylene glycol and polyethylene glycol assisted second antibody. Second antibody in a preprecipitated form was found to be readily adaptable as a reagent. Among the three methods of separation studied, the second antibody employed as a complex with primary antibody assisted by polyethylene glycol had superior separation without any specific criticality, characteristics as judged by the variations at various dose levels over an extended period and provided a practical procedure for routine assays. Akadémiai Kiadó, 1990 Rathinaswamy A. Radiophamaceuticals Operations, Board of Radiation and Isotope Technology, B.A.R.C. Turbhe Complex, 400705, New Bombay, (India) aut Nagvekar U. Radiophamaceuticals Operations, Board of Radiation and Isotope Technology, B.A.R.C. Turbhe Complex, 400705, New Bombay, (India) aut Borkute S. Radiophamaceuticals Operations, Board of Radiation and Isotope Technology, B.A.R.C. Turbhe Complex, 400705, New Bombay, (India) aut Balakrishnan S. Radiophamaceuticals Operations, Board of Radiation and Isotope Technology, B.A.R.C. Turbhe Complex, 400705, New Bombay, (India) aut Desai C. Radiophamaceuticals Operations, Board of Radiation and Isotope Technology, B.A.R.C. Turbhe Complex, 400705, New Bombay, (India) aut Journal of Radioanalytical and Nuclear Chemistry Kluwer Academic Publishers 139/2(1990-04-01), 215-221 0236-5731 139:2<215 1990 139 10967 https://doi.org/10.1007/BF02061805 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02061805 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Rathinaswamy A. Radiophamaceuticals Operations, Board of Radiation and Isotope Technology, B.A.R.C. Turbhe Complex, 400705, New Bombay, (India) aut NATIONALLICENCE 700 1- Nagvekar U. Radiophamaceuticals Operations, Board of Radiation and Isotope Technology, B.A.R.C. Turbhe Complex, 400705, New Bombay, (India) aut NATIONALLICENCE 700 1- Borkute S. Radiophamaceuticals Operations, Board of Radiation and Isotope Technology, B.A.R.C. Turbhe Complex, 400705, New Bombay, (India) aut NATIONALLICENCE 700 1- Balakrishnan S. Radiophamaceuticals Operations, Board of Radiation and Isotope Technology, B.A.R.C. Turbhe Complex, 400705, New Bombay, (India) aut NATIONALLICENCE 700 1- Desai C. Radiophamaceuticals Operations, Board of Radiation and Isotope Technology, B.A.R.C. Turbhe Complex, 400705, New Bombay, (India) aut NATIONALLICENCE 773 0- Journal of Radioanalytical and Nuclear Chemistry Kluwer Academic Publishers 139/2(1990-04-01), 215-221 0236-5731 139:2<215 1990 139 10967 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer