caa a22 4500 465733301 CHVBK 20180323111747.0 cr unu---uuuuu 170327e19900101xx s 000 0 eng 10.1007/BF02162190 doi (NATIONALLICENCE)springer-10.1007/BF02162190 Effect of the specific activity of the tracer on rat luteinizing hormone radioimmunoassay [Elektronische Daten] [E. Miholics, I. Sárándi, G. Tóth] Rat luteinizing hormone /LH/ was labelled with125I by the Chloramine T method.125I-LH, used as tracer in radioimmunoassay, was separated from the labelling reaction mixture by gel filtration. By using the proper protein/radioiodine ratio in the labelling reaction mixture the specific activity of125I-LH was adjusted to 2.5-20.5 MBq μg−1. The influence of the specific activity on the assay parameters as well as on the tracer stability was investigated. Akadémiai Kiadó, 1990 Miholics E. Institute of Isotopes of the, Hungarian Academy of Sciences, P.O. Box 77, H-1525, Budapest, Hungary aut Sárándi I. Institute of Isotopes of the, Hungarian Academy of Sciences, P.O. Box 77, H-1525, Budapest, Hungary aut Tóth G. Institute of Isotopes of the, Hungarian Academy of Sciences, P.O. Box 77, H-1525, Budapest, Hungary aut Journal of Radioanalytical and Nuclear Chemistry Kluwer Academic Publishers 144/2(1990-01-01), 79-87 0236-5731 144:2<79 1990 144 10967 https://doi.org/10.1007/BF02162190 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02162190 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Miholics E. Institute of Isotopes of the, Hungarian Academy of Sciences, P.O. Box 77, H-1525, Budapest, Hungary aut NATIONALLICENCE 700 1- Sárándi I. Institute of Isotopes of the, Hungarian Academy of Sciences, P.O. Box 77, H-1525, Budapest, Hungary aut NATIONALLICENCE 700 1- Tóth G. Institute of Isotopes of the, Hungarian Academy of Sciences, P.O. Box 77, H-1525, Budapest, Hungary aut NATIONALLICENCE 773 0- Journal of Radioanalytical and Nuclear Chemistry Kluwer Academic Publishers 144/2(1990-01-01), 79-87 0236-5731 144:2<79 1990 144 10967 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer