caa a22 4500 465733824 CHVBK 20180323111749.0 cr unu---uuuuu 170327e19900801xx s 000 0 eng 10.1007/BF00419942 doi (NATIONALLICENCE)springer-10.1007/BF00419942 The polymorphonuclear leukocyte: has it a role in fracture healing? [Elektronische Daten] [B. Grøgaard, B. Gerdin, O. Reikerås] Summary: The aim of the present study was to assess whether the presence of polymorphonuclear leukocytes in the first stage of fracture repair was of any importance. In anesthetized male Wistar rats, a transverse osteotomy was performed at midshaft in one femur and immediately stabilized by an intramedullary Kirschner nail. The animals were allowed unprotected weight bearing immediately. Nine animals were made neutropenic before operation by injection of a specific rat polymorphonuclear leukocyte antiserum (antineutrophil serum, ANS) raised in sheep. The number of circulating polymorphonuclear leukocytes were kept below 20% of their normal value for 72 h postoperatively by intraperitoneal injections of ANS every 12h. Control animals were injected with the same amount of normal sheep serum (NSS). All animals received cefuroxime (100 mg/kg day) concomitantly with the ANS injections. Six weeks after operation the animals were killed, the amount of callus formation was measured, and the bones were radiologically examined. The nails were then removed and both the healing fractures and the nonoperated femurs mechanically tested for bending. There were no differences in the amount of callus measured or in radiological healing. However, there was a significantly higher bending moment in femurs from animals treated with ANS than in those given NSS (P < 0.02). No differences were observed in rigidity or total energy absorption. Springer-Verlag, 1990 Grøgaard B. Department of Surgery, Orthopedic Section, Ullevål University Hospital, Kirkeveien 166, N-407, Oslo 4, Norway aut Gerdin B. Department of Surgery, University Hospital, Uppsala, Sweden aut Reikerås O. Department of Surgery, Orthopedic Section, Ullevål University Hospital, Kirkeveien 166, N-407, Oslo 4, Norway aut Archives of Orthopaedic and Trauma Surgery Springer-Verlag 109/5(1990-08-01), 268-271 0936-8051 109:5<268 1990 109 402 https://doi.org/10.1007/BF00419942 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00419942 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Grøgaard B. Department of Surgery, Orthopedic Section, Ullevål University Hospital, Kirkeveien 166, N-407, Oslo 4, Norway aut NATIONALLICENCE 700 1- Gerdin B. Department of Surgery, University Hospital, Uppsala, Sweden aut NATIONALLICENCE 700 1- Reikerås O. Department of Surgery, Orthopedic Section, Ullevål University Hospital, Kirkeveien 166, N-407, Oslo 4, Norway aut NATIONALLICENCE 773 0- Archives of Orthopaedic and Trauma Surgery Springer-Verlag 109/5(1990-08-01), 268-271 0936-8051 109:5<268 1990 109 402 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer