caa a22 4500 465734197 CHVBK 20180323111750.0 cr unu---uuuuu 170327e19900101xx s 000 0 eng 10.1007/BF01050405 doi (NATIONALLICENCE)springer-10.1007/BF01050405 Specificity of human antibodies against galα1-3gal carbohydrate epitope and distinction from natural antibodies reacting with galα1-2gal or galα1-4 gal [Elektronische Daten] [JÖrgen Wieslander, Olle Månsson, Elisabeth Kallin, Armando Gabreilli, Hans Nowack, Rupert Timpl] Antibodies against galactosyl-α1-3-galactose epitopes were characterized in normal and patient sera by radioimmunoassay binding to mouse laminin and oligosaccharide inhibition. Binding was strictly dependent on α-linked galactose in a terminal position. Reduced affinities were observed for digalactoses with α(1-2)-, α(1-6)- and α(1-4)-linkages and for the blood group B epitope, Galα1-3(Fucα1-2)Gal. Conformational models of various active and inactive oligosaccharides provided a clearer picture of the epitope requirements for the observed antibody specificity. Some antibody heterogeneity was detected by comparing individual sera and by hapten elution from a laminin adsorbent. New assays were developed with synthetic Galα1-3Gal-albumin conjugates and these were shown to be more sensitive than assays with mouse laminin. Two more ubiquitous human antibodies could be detected with Galα1-2Gal and Galα1-4Gal conjugates. They were distinct from Galα1-3Gal-specific antibodies as shown by carbohydrate inhibition. This demonstrates a considerable diversity in the recognition of α-linked galactose epitopes by natural antibodies. Glycoconjugate Journal, 1990 natural antibodies nationallicence carbohydrate epitopes nationallicence Wieslander JÖrgen BioCarb AB, S-223 70, Lund, Sweden aut Månsson Olle BioCarb AB, S-223 70, Lund, Sweden aut Kallin Elisabeth BioCarb AB, S-223 70, Lund, Sweden aut Gabreilli Armando Max-Planck-Institut für Biochemie, D-8033, Martinsried, Germany aut Nowack Hans Max-Planck-Institut für Biochemie, D-8033, Martinsried, Germany aut Timpl Rupert Max-Planck-Institut für Biochemie, D-8033, Martinsried, Germany aut Glycoconjugate Journal Kluwer Academic Publishers 7/1(1990-01-01), 85-100 0282-0080 7:1<85 1990 7 10719 https://doi.org/10.1007/BF01050405 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF01050405 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Wieslander JÖrgen BioCarb AB, S-223 70, Lund, Sweden aut NATIONALLICENCE 700 1- Månsson Olle BioCarb AB, S-223 70, Lund, Sweden aut NATIONALLICENCE 700 1- Kallin Elisabeth BioCarb AB, S-223 70, Lund, Sweden aut NATIONALLICENCE 700 1- Gabreilli Armando Max-Planck-Institut für Biochemie, D-8033, Martinsried, Germany aut NATIONALLICENCE 700 1- Nowack Hans Max-Planck-Institut für Biochemie, D-8033, Martinsried, Germany aut NATIONALLICENCE 700 1- Timpl Rupert Max-Planck-Institut für Biochemie, D-8033, Martinsried, Germany aut NATIONALLICENCE 773 0- Glycoconjugate Journal Kluwer Academic Publishers 7/1(1990-01-01), 85-100 0282-0080 7:1<85 1990 7 10719 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer