caa a22 4500 465734278 CHVBK 20180323111750.0 cr unu---uuuuu 170327e19901101xx s 000 0 eng 10.1007/BF01189080 doi (NATIONALLICENCE)springer-10.1007/BF01189080 Glycolipid- and glycoprotein-based blood group A antigen expression in human thrombocytes. A1/A2 difference [Elektronische Daten] [Jan Holgersson, Michael Breimer, Anita Jacobsson, Lola Svensson, Ami Ulfvin, Bo Samuelsson] Total non-acid glycolipid fractions and total sodium dodecylsulphate (SDS) solubilized protein fractions were isolated from human thrombocytes obtained from single human donors having different blood group A1/A2 phenotypes. The blood group A glycolipid antigens were characterized by immunostaining of thin layer plates with different monoclonal anti-A antibodies. The glycoproteins carrying blood group A epitopes were identified by SDS-PAGE and Western blot analysis using a monoclonal anti-A antibody. Blood group A glycolipid antigens were found in both A1 and A2 thrombocytes but the A2 individuals expressed at least ten times less A glycolipids compared to the A1 individuals. Expression of A type 3/4 chain and small amounts of A type 1 chain glycolipids were seen in thrombocytes of both A1 and A2 individuals, while the type 2 chain A glycolipids appeared to be missing from the A2 thrombocytes. Blood group A reactive glycoproteins were only found in thrombocytes of A1 individuals and could not be detected in A2 individuals or a blood group O individual. The major blood group A glycoprotein were found as a double band migrating in the 130 kDa region. Glycoconjugate Journal, 1990 thrombocytes nationallicence blood group A nationallicence glycosphingolipid nationallicence glycoprotein nationallicence SDS : sodium dodecyl sulfate nationallicence PAGE : polyacrylamide gel electrophoresis nationallicence HPTLC : high performance thin layer chromatography nationallicence CBB : Coomassie brilliant blue nationallicence GVH : graft versus host nationallicence Holgersson Jan Department of Medical Biochemistry, University of Göteborg, P.O. Box 33031, S-400 33, Göteborg, Sweden aut Breimer Michael Department of Surgery, Sahlgren's Hospital, University of Göteborg, S-413 45, Göteborg, Sweden aut Jacobsson Anita Department of Medical Biochemistry, University of Göteborg, P.O. Box 33031, S-400 33, Göteborg, Sweden aut Svensson Lola Regional Blood Centre, Sahlgren's Hospital, University of Göteborg, S-413 45, Göteborg, Sweden aut Ulfvin Ami Regional Blood Centre, Sahlgren's Hospital, University of Göteborg, S-413 45, Göteborg, Sweden aut Samuelsson Bo Department of Medical Biochemistry, University of Göteborg, P.O. Box 33031, S-400 33, Göteborg, Sweden aut Glycoconjugate Journal Kluwer Academic Publishers 7/6(1990-11-01), 601-608 0282-0080 7:6<601 1990 7 10719 https://doi.org/10.1007/BF01189080 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF01189080 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Holgersson Jan Department of Medical Biochemistry, University of Göteborg, P.O. Box 33031, S-400 33, Göteborg, Sweden aut NATIONALLICENCE 700 1- Breimer Michael Department of Surgery, Sahlgren's Hospital, University of Göteborg, S-413 45, Göteborg, Sweden aut NATIONALLICENCE 700 1- Jacobsson Anita Department of Medical Biochemistry, University of Göteborg, P.O. Box 33031, S-400 33, Göteborg, Sweden aut NATIONALLICENCE 700 1- Svensson Lola Regional Blood Centre, Sahlgren's Hospital, University of Göteborg, S-413 45, Göteborg, Sweden aut NATIONALLICENCE 700 1- Ulfvin Ami Regional Blood Centre, Sahlgren's Hospital, University of Göteborg, S-413 45, Göteborg, Sweden aut NATIONALLICENCE 700 1- Samuelsson Bo Department of Medical Biochemistry, University of Göteborg, P.O. Box 33031, S-400 33, Göteborg, Sweden aut NATIONALLICENCE 773 0- Glycoconjugate Journal Kluwer Academic Publishers 7/6(1990-11-01), 601-608 0282-0080 7:6<601 1990 7 10719 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer