caa a22 4500 465734308 CHVBK 20180323111750.0 cr unu---uuuuu 170327e19901101xx s 000 0 eng 10.1007/BF01189076 doi (NATIONALLICENCE)springer-10.1007/BF01189076 Endothelial heparan sulphate: Compositional analysis and comparison of chains from different proteoglycan populations [Elektronische Daten] [Anders Lindblom, Lars-Åke Fransson] From cultures of human umbilical vein endothelial cells incubated with3H-glucosamine or35S-sulphate, we have purified three heparan sulphate proteoglycans: 1) a low density (1.31 g/ml) proteoglycan from the cell extract, 2) a low density proteoglycan from the medium, and 3) a high density (>1.4 g/ml) proteoglycan from the medium. The disaccharide composition of heparan sulphate chains from the low density proteoglycan of the medium was examined, using specific chemical and enzymic degradations followed by gel chromatography and strong anion exchange HPLC. Chains released from each of the different proteoglycan populations were then compared by gel chromatography and gradient polyacrylamide gel electrophoresis before and after various specific degradations. The results indicate that heparan sulphate from human endothelial cells are large polymers (MW>50,000) of low overall sulphation (32-35%N-sulphated glucosamine and an N/O-linked sulphate ratio of 2.0) with rare and solitary heparin-like disaccharides. Heparan sulphate from the different proteoglycan populations appeared to have similar structure except that chains from the high density fraction were larger polymers. Glycoconjugate Journal, 1990 proteoglycans nationallicence heparan sulphate nationallicence endothelial cells nationallicence oligosaccharide mapping nationallicence HSPG : heparan sulphate proteoglycan nationallicence DSPG : dermatan sulphate proteoglycan nationallicence GlcNAc(6S) : N-acetylglucosamine 6-sulphate nationallicence GlcNAc6R : glucosamine with either-OH or-OSO3 at C-6 nationallicence GlcNR : glucosamine with either-SO3 or-COCH3 as N-substituent nationallicence GlcNSO3 : N-sulphated glucosamine nationallicence GlcNSO3(3S) : N-sulphated glucosamine 3-sulphate nationallicence GlcA : d-glucuronic acid nationallicence IdoA : l-iduronic acid nationallicence IdoA(2S) : iduronic acid 2-sulphate nationallicence HexA : hexuronic acid nationallicence DHexA : hexuronic acid with a 4,5-double bond nationallicence Xyl : xylose nationallicence SAX : strong anion exchange nationallicence d.p. : degree of polymerization (a disaccharide has d.p.=1 etc) nationallicence AUFS : absorbance units full scale nationallicence Lindblom Anders Department of Physiological Chemistry, University of Lund, P.O. Box 94, S-221 00, Lund, Sweden aut Fransson Lars-Åke Department of Physiological Chemistry, University of Lund, P.O. Box 94, S-221 00, Lund, Sweden aut Glycoconjugate Journal Kluwer Academic Publishers 7/6(1990-11-01), 545-562 0282-0080 7:6<545 1990 7 10719 https://doi.org/10.1007/BF01189076 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF01189076 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Lindblom Anders Department of Physiological Chemistry, University of Lund, P.O. Box 94, S-221 00, Lund, Sweden aut NATIONALLICENCE 700 1- Fransson Lars-Åke Department of Physiological Chemistry, University of Lund, P.O. Box 94, S-221 00, Lund, Sweden aut NATIONALLICENCE 773 0- Glycoconjugate Journal Kluwer Academic Publishers 7/6(1990-11-01), 545-562 0282-0080 7:6<545 1990 7 10719 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer